CD31/PECAM-1 Antibody (JC/70A) - BSA Free

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 10 applications: ELISA, FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IHC-WhMt, Immunocytochemistry/ Immunofluorescence, WB.

Showing Publications 1 - 10 of 54. Show All 54 Publications. Collapse Publications.

Publications using NB600-562	Applications	Species
Zhang K, Jin D, Zhao X et al. HIF-1?-Induced Mitophagy Regulates the Regenerative Outcomes of Stem Cells in Fat Transplantation Cell transplantation 2023-11-27 [PMID: 38009534] (IHC, Mouse)	IHC	Mouse
Brady EL, Prado O, Johansson F et al. Engineered tissue vascularization and engraftment depends on host model Scientific reports 2023-02-03 [PMID: 36737618] (IHC-WhMt)	IHC-WhMt
Brady E Host-Dependent Vascularization in Cardiac Tissue Engineering Thesis 1905-07-13 (WB)	WB
Brady E Host-Dependent Vascularization in Cardiac Tissue Engineering Thesis Jul 13 1905 (WB)	WB
Sewoom Baek, Hyun-Su Ha, Jeong Su Park, Min Jeong Cho, Hye-Seon Kim, Seung Eun Yu, Seyong Chung, Chansik Kim, Jueun Kim, Ji Youn Lee, Yerin Lee, Hyunjae Kim, Yujin Nam, Sungwoo Cho, Kyubae Lee, Ja Kyung Yoon, Jin Sub Choi, Dai Hoon Han, Hak-Joon Sung Chip collection of hepatocellular carcinoma based on O 2 heterogeneity from patient tissue Nature Communications 2024-06-15 [PMID: 38879551]
Rosalia Lira, César Luna-Rivero, Francina Valezka Morales-Bolaños, José Luis Sandoval-Gutiérrez, Elsa Romelia Moreno-Verduzco, Angélica Maldonado-Rodriguez, Jesús Miguel Torres-Flores, Martha Yocupicio-Monroy, Edgar E. Sevilla-Reyes Case Report of a Young Adult with Fatal COVID-19 and Abundant SARS-CoV-2 Nucleocapsid Protein and Lipofuscin Accumulation in Tissues Heliyon 2023-12-10 [PMID: 38173536]
Zheng M, Wang Y, Fu F et al. Radioimmunotherapy Targeting B7-H3 in situ glioma models enhanced antitumor efficacy by Reconstructing the tumor microenvironment International Journal of Biological Sciences 2023-08-15 [PMID: 37705739] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Dhumale P, Nielsen JV, Hansen ACS et al. CD31 defines a subpopulation of human adipose-derived regenerative cells with potent angiogenic effects Sci Rep 2023-09-01 [PMID: 37658225] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Ball EE, Weiss CM, Liu H et al. Severe Acute Respiratory Syndrome Coronavirus 2 Vasculopathy in a Syrian Golden Hamster Model The American journal of pathology 2023-03-10 [PMID: 36906263] (IHC-Fr, Human) Details: 1:20 Dilution	IHC-Fr	Human
Xie C, Liang R, Ye J et al. High-efficient engineering of osteo-callus organoids for rapid bone regeneration within one month Biomaterials 2022-08-19 [PMID: 36031458]
Xiao W, Chen W, Wang Y et al. Recombinant DT beta 4-inspired porous 3D vascular graft enhanced antithrombogenicity and recruited circulating CD93+/CD34+ cells for endothelialization Science advances 2022-07-15 [PMID: 35857526] (ICC/IF, Rat)	ICC/IF	Rat
Lee M, Kim H, Kwak I et al. Immunohistochemical Analysis of Postburn Scars following Treatment Using Dermal Substitutes Analytical Cellular Pathology 2022-02-25 [PMID: 35251908]
Gan F, Liu L, Zhou Q et al. Effects of adipose-derived stromal cells and endothelial progenitor cells on adipose transplant survival and angiogenesis PloS one 2022-01-13 [PMID: 35025920] (ICC/IF, Rabbit)	ICC/IF	Rabbit
Yu Y, Wang X, Jia X Et al. Aptamer Probes Labeled with Lanthanide-Doped Carbon Nanodots Permit Dual-Modal Fluorescence and Mass Cytometric Imaging Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2021-11-01 [PMID: 34719883] (IHC-P, Human)	IHC-P	Human
Guzman RA, Maruyama M, Moeinzadeh S et al. The effect of genetically modified platelet-derived growth factor-BB over-expressing mesenchymal stromal cells during core decompression for steroid-associated osteonecrosis of the femoral head in rabbits Stem cell research & therapy 2021-09-15 [PMID: 34526115] (IHC-Fr, Rabbit)	IHC-Fr	Rabbit
Richard J. Jabbour, Thomas J. Owen, Pragati Pandey, Marina Reinsch, Brian Wang, Oisín King, Liam Steven Couch, Dafni Pantou, David S. Pitcher, Rasheda A. Chowdhury, Fotios G. Pitoulis, Balvinder S. Handa, Worrapong Kit-Anan, Filippo Perbellini, Rachel C. Myles, Daniel J. Stuckey, Michael Dunne, Mayooran Shanmuganathan, Nicholas S. Peters, Fu Siong Ng, Florian Weinberger, Cesare M. Terracciano, Godfrey L. Smith, Thomas Eschenhagen, Sian E. Harding In vivo grafting of large engineered heart tissue patches for cardiac repair JCI Insight 2021-08-09 [PMID: 34369384]
Maruyama M, Moeinzadeh S, Guzman RA et al. The efficacy of lapine preconditioned or genetically modified IL4 over-expressing bone marrow-derived mesenchymal stromal cells in corticosteroid-associated osteonecrosis of the femoral head in rabbits Biomaterials 2021-06-21 [PMID: 34186237]
Raghavan S, Kenchappa DB,Leo MD SARS-CoV-2 Spike Protein Induces Degradation of Junctional Proteins That Maintain Endothelial Barrier Integrity Frontiers in cardiovascular medicine 2021-06-11 [PMID: 34179146]
Pitale PM, Saltykova IV, Adu-Agyeiwaah Y et al. Tribbles Homolog 3 Mediates the Development and Progression of Diabetic Retinopathy Diabetes 2021-05-11 [PMID: 33975909]
Khan M, Ashraf M Human iPS Cells Derived Skeletal Muscle Progenitor Cells Promote Myoangiogenesis and Restore Dystrophin in Duchenne Muscular Dystrophic Mice Stem Cell Res Ther 2021-02-13 [PMID: 33579366] (IHC-Fr, Human)	IHC-Fr	Human
Woloshuk A, Khochare S, Almulhim A et al. In situ classification of cell types in human kidney tissue using 3D nuclear staining Cytometry A 2020-11-30 [PMID: 33252180]
Maruyama M, Lin T, Kaminow NI et al. The efficacy of core decompression for steroidassociated osteonecrosisof the femoral head in rabbits J Orthop Res 2020-10-23 [PMID: 33095462]
Kuang XY, Ren Y, Chen C et al. Quantitative analysis for the differences in vasculogenic activity and sensitivity to angiogenic stimulants between human glioma cells and normal endothelial cells Brain Res. 2020-08-28 [PMID: 32866544] (IF/IHC, Mouse)	IF/IHC	Mouse
Tone T, Shimizu Y, Saito H et al. In vivo behavior of untreated and compressed concentrated growth factors as biomaterials in rabbits Dent Mater J 2020-08-07 [PMID: 32779604] (IHC-P, Rabbit)	IHC-P	Rabbit
Brady El, Kirby Ma, Olszewski E et Al. Guided vascularization in the rat heart leads to transient vessel patterning APL Bioeng. [PMID: 32161835] (IHC-P, Human)	IHC-P	Human
Xu G, Yamamoto N, Nojima T, et al. The process of bone regeneration from devitalization to revitalization after pedicle freezing with immunohistochemical and histological examination in rabbits Cryobiology 2019-12-22 [PMID: 31875528] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Quintela-Fandino M, Morales S, Cortes-Salgado A, Manso L et al. Randomized Phase 0/I Trial of the Mitochondrial Inhibitor ME-344 or Placebo Added to Bevacizumab in Early HER2-Negative Breast Cancer Clin Cancer Res 2019-10-11 [PMID: 31597662] (IHC-P, Human)	IHC-P	Human
Kimball EE, Sayce L, Powell M et al. Different Vibratory Conditions Elicit Different Structural and Biological Vocal Fold Changes in an In-Vivo Rabbit Model of Phonation J Voice 2019-09-18 [PMID: 31542239] (IHC-Fr, Rabbit)	IHC-Fr	Rabbit
Damond N, Engler S, Zanotelli VRT et al. A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry Cell Metab. 2019-01-11 [PMID: 30713109] (IF/IHC, Human)	IF/IHC	Human
Lee SJ, Won JE, Han C et al. Development of a three-dimensionally printed scaffold grafted with bone forming peptide-1 for enhanced bone regeneration with in vitro and in vivo evaluations J Colloid Interface Sci 2018-12-28 [PMID: 30611042] (IHC-P, Rabbit)	IHC-P	Rabbit
Wilson C L, Stephenson S E et al. Characterization of human PDGFR-beta-positive pericytes from IPF and non-IPF lungs. Am J Physiol Lung Cell Mol Physiol 2018-01-12 [PMID: 30335500] (ICC/IF, Human)	ICC/IF	Human
Miyanaga T, Ueda Y, Miyanaga A et al. Angiogenesis after administration of basic fibroblast growth factor induces proliferation and differentiation of mesenchymal stem cells in elastic perichondrium in an in vivo model: mini review of three sequential republication-abridged reports. Cell. Mol. Biol. Lett. 2018-10-05 [PMID: 30323846] (IHC-P, Rabbit)	IHC-P	Rabbit
Hathi D, DeLassus E, Achilefu S et al. Imaging melphalan therapy response in preclinical extramedullary myeloma with 18F-FDOPA and 18F-FDG PET J. Nucl. Med. 2018-04-26 [PMID: 29700126] (Mouse)		Mouse
Vlachogiannis G, Hedayat S, Vatsiou A et al. Patient-derived organoids model treatment response of metastatic gastrointestinal cancers. Science 2018-02-23 [PMID: 29472484]
He Y, Mei L, Jin Y, Li X. Overexpression of hepatocyte growth factor mRNA induced by gene transfer attenuates neointimal hyperplasia after balloon injury. Hum. Gene Ther. 2018-01-30 [PMID: 29382231] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Ishihara T, Yamashita H, Sakurai T et al. Morphological analysis of patchy thickening and reddish discoloration of active hair growth areas in the skin of New Zealand White rabbits J Toxicol Pathol. 2017-08-18 [PMID: 29097841] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Lin CC, Lin AT, Yang AH, Chen KK. Microvascular Injury in Ketamine-Induced Bladder Dysfunction. PLoS ONE. 2016-08-17 [PMID: 27529746] (ICC/IF)	ICC/IF
Strilic B, Yang L, Albarran-Juarez J et al. Tumour-cell-induced endothelial cell necroptosis via death receptor 6 promotes metastasis. Nature. 2016-08-11 [PMID: 27487218] (IF/IHC, Human)	IF/IHC	Human
Kuang XY, Jiang XF, Chen C et al. Expressions of glia maturation factor-B by tumor cells and endothelia correlate with neovascularization and poor prognosis in human glioma. Oncotarget. 2016-12-27 [PMID: 26515590] (IF/IHC, Human)	IF/IHC	Human
Song Y, Zheng J, Yan M et al. The Effects of Irreversible Electroporation on the Achilles Tendon: An Experimental Study in a Rabbit Model PLoS ONE 2015-06-27 [PMID: 26114962] (IHC-P, Rabbit) Details: CD31/PECAM-1 antibody (clone JC/70A) was used at 1:50 dilution for IHC-P analysis of irreversible electroporation/IRE-ablated segment, a distal segment and a proximal segment of muscle-tendon complexes from Rabbits (Fig 8).	IHC-P	Rabbit
Sun Dongchong, Yang Yong, Wei Zhitao et al. Engineering of pre-vascularized urethral patch with muscle flaps and hypoxia-activated hUCMSCs improves its therapeutic outcome. J Cell Mol Med. 2014-01-25 [PMID: 24460735] (IF/IHC)	IF/IHC
Song X, Wang J, Zheng T et al. LBH589 Inhibits proliferation and metastasis of hepatocellular carcinoma via inhibition of gankyrin/stat3/akt pathway. Mol Cancer. 2013-10-05 [PMID: 24093956] (WB, Human)	WB	Human
Kalen M, Heikura T, Karvinen H et al. Gamma-secretase inhibitor treatment promotes VEGF-A-driven blood vessel growth and vascular leakage but disrupts neovascular perfusion. PLoS One. 2011-04-14 [PMID: 21533193] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Longatto Filho A, Oliveira TG, Pinheiro C et al. How useful is the assessment of lymphatic vascular density in oral carcinoma prognosis? World J Surg Oncol. 2007-12-11 [PMID: 18072963] (IF/IHC, Human)	IF/IHC	Human
Ding MX, Lin XQ, Fu XY Zhang N, Li JC. Expression of vascular endothelial growth factor-C and angiogenesis in esophageal squamous cell carcinoma World J Gastroenterol. 2006-07-28 [PMID: 16874878] (IF/IHC, Human)	IF/IHC	Human
Elaqoz S, Eqilmez R, Koyuncu A et al. The intratumoral microvessel density and expression of bFGF and nm23-H1 in colorectal cancer. Pathol Oncol Res. 2006-01-01 [PMID: 16554912] (IF/IHC, Human)	IF/IHC	Human
Zurita AJ, Troncoso P, Cardo-Vila M et al. Combinatorial screenings in patients: the interleukin-11 receptor alpha as a candidate target in the progression of human prostate cancer. Cancer Res. 2004-01-15 [PMID: 14744752] (IF/IHC, Human)	IF/IHC	Human
Hambrecht R, Adams V, Erbs S. Regular physical activity improves endothelial function in patients with coronary artery disease by increasing phosphorylation of endothelial nitric oxide synthase. Circulation. 2003-07-01 [PMID: 12810615] (IF/IHC, Human)	IF/IHC	Human
Ikpeazu C, Davidson MK, halteman D, et al. Donor origin of circulating endothelial progenitors after allogeneic bone marrow transplantation. Biol Blood Marrow Transplant. 2000-01-01 [PMID: 10905767] (IF/IHC, Human)	IF/IHC	Human
Tjalma W, Van Marck E, Weyler J et al. Quantification and prognostic relevance of angiogenic parameters in invasive cervical cancer. Br J Cancer. 1998-07-01 [PMID: 9683289] (IF/IHC, Human)	IF/IHC	Human
Bird IN, Spragg JH, Ager A, Matthews N. Studies of lymphocyte transendothelial migration: analysis of migrated cell phenotypes with regard to CD31 (PECAM-1), CD45RA and CD45RO. Immunology. 1993-12-01 [PMID: 8307606] (ELISA, Human)	ELISA	Human
Chung S, Kim S, Lee K et al. In Situ Reprogrammings of Splenic CD11b + Cells by Nano?Hypoxia to Promote Inflamed Damage Site?Specific Angiogenesis Advanced Functional Materials 2023-05-01
Hun Kim J, Liu Q, Lee U et al. Antioxidant-Coated multifunctional whitlockite scaffold for the treatment of Steroid-Induced osteonecrosis of the femoral head Chemical Engineering Journal 2023-10-01 (IHC, Rabbit)	IHC	Rabbit
Reymond N, Imbert AM, Devilard E et al. DNAM-1 and PVR regulate monocyte migration through endothelial junctions. J Exp Med. 2004-05-17 [PMID: 15136589] (FLOW, IF/IHC, Human)	FLOW, IF/IHC	Human
Show All 54 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	3 reviewed by: Verified Customer	IHC-Fr	Mouse	05/09/2019	View Summary Application Immunohistochemistry-Frozen Sample Tested Mouse brain Species Mouse Lot D
	4 reviewed by: Verified Customer	ICC	Feline	05/26/2017	View Summary Application Immunocytochemistry Sample Tested Endothelial Species Feline Lot C-1
Enlarge	5 reviewed by: Verified Customer	IHC-P	Mouse	08/12/2016	View Summary Application Immunohistochemistry-Paraffin Sample Tested non-ischemic and ischemic gastrocnemius muscle Species Mouse Lot A
Enlarge	5 reviewed by: Verified Customer	IHC-Fr	Other	04/28/2014	View Summary Application Immunohistochemistry-Frozen Sample Tested Rabbit Heart Species Other Lot A-1

Array NB600-562

• CD31/PECAM-1 Antibodies
• CD31/PECAM-1 Antibody Pair
• CD31/PECAM-1 ELISA Kits
• CD31/PECAM-1 Lysate
• CD31/PECAM-1 Protein
• CD31/PECAM-1 ELISAs
• CD31/PECAM-1 Proteins and Enzymes