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CD3 Antibody (Hu113) [Unconjugated]

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Human PBMCs were stained with (A) Human CD3 (Teplizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9929) or (B) no primary antibody, followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) and ...read more
Human Jurkat cell line was stained with Human CD3 (Teplizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9929, filled histogram) or Normal Human IgG control antibody (Catalog # 1-001-A, open histogram) followed by ...read more
Biosimilar antibody development at Bio-Techne includes in silico design and engineering to generate both light chain (lc) and heavy chain (hc) sequences for each antibody. After transfection of the dual expression ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
Hu113
Clonality
Monoclonal
Host
Human
Conjugate
Unconjugated

Order Details

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CD3 Antibody (Hu113) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody For Research Use Only
Immunogen
Human CD3
Specificity
Detects Human CD3 based on Teplizumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Teplizumab. This product is for research use only.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Human
Gene
CD3E
Purity Statement
Protein A or G purified from cell culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD3 Antibody (Hu113) [Unconjugated]

  • CD_antigen: CD3e
  • CD3 antigen, delta subunit
  • CD3
  • CD3d antigen
  • CD3d antigen, delta polypeptide (TiT3 complex)
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3-DELTA
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3e
  • CD3-epsilon
  • CD3g antigen
  • CD3g antigen, gamma polypeptide (TiT3 complex)
  • CD3g molecule, epsilon (CD3-TCR complex)
  • CD3g molecule, gamma (CD3-TCR complex)
  • CD3G
  • CD3-GAMMA
  • FLJ17620
  • FLJ17664
  • FLJ18683
  • FLJ79544
  • FLJ94613
  • IMD18
  • MGC138597
  • T3DOKT3, delta chain
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell receptor T3 delta chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 delta chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Background

Teplizumab is a biosimilar directed against the extracellular domain of CD3. This antibody can be used in flow cytometry or various assay format to measure CD3 expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for CD3 Antibody (MAB9929) (0)

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Isotype Controls

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Bioinformatics

Gene Symbol CD3E