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Caspase-4 Antibody

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Western Blot: Caspase-4 Antibody [NBP3-13397] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase-4 antibody (NBP3-13397) ...read more
Immunohistochemistry-Paraffin: Caspase-4 Antibody [NBP3-13397] - Rat kidney. Caspase-4 stained by Caspase-4 antibody (NBP3-13397) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Biological Strategies: Western Blot: Caspase-4 Antibody [NBP3-13397] - THP-1 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase-4 (cleaved Gln81) antibody ...read more
Immunohistochemistry-Paraffin: Caspase-4 Antibody [NBP3-13397] - Mouse duodenum. Caspase-4 stained by Caspase-4 antibody (NBP3-13397) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Caspase-4 Antibody [NBP3-13397] -Untreated (-) and treated (+) THP-1 whole cell extract and conditioned medium (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody ( ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

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Caspase-4 Antibody Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Caspase-4. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP4
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for Caspase-4 Antibody

  • apoptotic cysteine protease Mih1/TX
  • CASP4
  • CASP-4
  • caspase 4, apoptosis-related cysteine peptidase
  • caspase 4, apoptosis-related cysteine protease
  • Caspase4
  • Caspase-4
  • EC 3.4.22
  • EC 3.4.22.57
  • ICE(rel)II
  • ICE(rel)-II
  • ICEREL-II
  • ICH2
  • ICH-2
  • Mih1/TX
  • Protease ICH-2
  • Protease TX
  • TX

Background

Caspase 4 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-4 Antibody (NBP3-13397) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Caspase-4 Products

Research Areas for Caspase-4 Antibody (NBP3-13397)

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Blogs on Caspase-4.

The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

Caspase-4 - a human protease with roles in inflammation and immunity
Caspases are a family of cysteine-aspartic acid proteases that cleave caspase proenzymes as well as other protein substrates. Caspases are well known for their role in apoptosis, but they also play a significant role in other cellular processes inc...  Read full blog post.

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Bioinformatics

Gene Symbol CASP4