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Carm1 Antibody (CARM1/7426) - Azide and BSA Free

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Formalin-fixed, paraffin-embedded human prostate stained with Carm1 antibody (CARM1/7426). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human prostate stained with Carm1 antibody (CARM1/7426). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Carm1 antibody (CARM1/7426). Inset: PBS instead of primary antibody; secondary only negative control.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
CARM1/7426
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-20909AF350
Alexa Fluor 405
NBP3-20909AF405
Alexa Fluor 488
NBP3-20909AF488
Alexa Fluor 532
NBP3-20909AF532
Alexa Fluor 594
NBP3-20909AF594
Alexa Fluor 647
NBP3-20909AF647
Alexa Fluor 700
NBP3-20909AF700
Alexa Fluor 750
NBP3-20909AF750
Allophycocyanin
NBP3-20909APC
Biotin
NBP3-20909B
CoraFluor 1
NBP3-20909CL1
DyLight 350
NBP3-20909UV
DyLight 405
NBP3-20909V
DyLight 488
NBP3-20909G
DyLight 550
NBP3-20909R
DyLight 594
NBP3-20909DL594
DyLight 650
NBP3-20909C
DyLight 680
NBP3-20909FR
DyLight 755
NBP3-20909IR
FITC
NBP3-20909F
HRP
NBP3-20909H
Janelia Fluor 525
NBP3-20909JF525
Janelia Fluor 549
NBP3-20909JF549
Janelia Fluor 585
NBP3-20909JF585
Janelia Fluor 635
NBP3-20909JF635
Janelia Fluor 646
NBP3-20909JF646
Janelia Fluor 669
NBP3-20909JF669
PE
NBP3-20909PE
PerCP
NBP3-20909PCP
Unconjugated
NBP3-20591-100ug
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-20591-100ug
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Carm1 Antibody (CARM1/7426) - Azide and BSA Free Summary

Description
Positive Controls: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Immunogen
Recombinant fragment (around aa200-400) of human Carm1 protein (exact sequence is proprietary)
Localization
Nucleus. Cytoplasm.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
CARM1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
10mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Carm1 Antibody (CARM1/7426) - Azide and BSA Free

  • Carm1
  • coactivator-associated arginine methyltransferase 1EC 2.1.1.-
  • EC 2.1.1
  • histone-arginine methyltransferase CARM1
  • PRMT4
  • PRMT4EC 2.1.1.125
  • Protein arginine N-methyltransferase 4

Background

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 (PubMed 11724789)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CARM1