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Carbohydrate Sulfotransferase 5/CHST5 Antibody

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Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbohydrate Sulfotransferase 5/CHST5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbohydrate Sulfotransferase 5/CHST5 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbohydrate Sulfotransferase 5/CHST5 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5446-SP
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Carbohydrate Sulfotransferase 5/CHST5 Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 5/CHST5
Ser28-Arg390
Accession # Q9GZS9
Specificity
Detects human Carbohydrate Sulfotransferase 5/CHST5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CHST1, rhCHST2, rmCHST5, and rmCHST7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHST5
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbohydrate Sulfotransferase 5/CHST5 Antibody

  • carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5
  • Carbohydrate Sulfotransferase 5
  • CHST5
  • GlcNAc6ST3
  • gn6st-3
  • GST4-alpha
  • hIGn6ST
  • I-GlcNAc6ST

Background

CHST5 (Carbohydrate sulfotransferase 5; also GlcNAc6ST-3, I-GlcNAc6ST and GST4 alpha ) is likely a 53‑56 kDa member of the sulfotransfease family of enzymes. It is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium. It appears to act only on short carbohydrates and transfers sulfate to position # 6 of mucin-associated GlcNAc. Human CHST5 is a 390 amino acid (aa) type II transmembrane protein. It contains a short cytoplasmic segment (aa 1‑9) and a long luminal domain (aa 28‑390) that contains an enzymatic region (aa 180‑358). There is one alternate start site that is 22 aa upstream of the standard site. Humans have a GST4-alpha and -beta gene, the result of gene duplication. Over aa 28‑390, these two gene products share 87% aa identity. Mouse has only one GST4 gene, and it is functionally related to human GST4-beta . Over aa 28‑390, human GST4‑ alpha /CHST5 is 78% aa identical to mouse GST4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CHST5
Uniprot