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Brg1 Antibody - BSA Free

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Western Blot: Brg1 Antibody [NBP2-41270] - Analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: Brg1 Antibody [NBP2-41270] - Mouse testis tissue with SMARCA4 antibody at 2 ug/ml.
Immunohistochemistry: Brg1 Antibody [NBP2-41270] - Rat brain tissue with BRG1 antibody at 5 ug/mL.
Immunohistochemistry-Paraffin: Brg1 Antibody [NBP2-41270] - Rat brain tissue with BRG1 antibody at 5 ug/mL.
Immunohistochemistry: Brg1 Antibody [NBP2-41270] - Mouse testis tissue with SMARCA4 antibody at 20 ug/ml.
Immunohistochemistry: Brg1 Antibody - BSA Free [NBP2-41270] - Immunohistochemistry of Brg1 in rat brain tissue with Brg1 antibody at 5 u/mL.
Western Blot: Brg1 Antibody - BSA Free [NBP2-41270] - Western blot analysis of Brg1 in SK-N-SH cell lysate with Brg1 antibody at 1 u/ml.
Immunohistochemistry: Brg1 Antibody - BSA Free [NBP2-41270] - Immunohistochemistry of Brg1 in rat brain tissue with Brg1 antibody at 5 u/mL.
Western Blot: Brg1 Antibody - BSA Free [NBP2-41270] - Western blot analysis of Brg1 in SK-N-SH cell lysate with Brg1 antibody at 1 u/ml.
Immunohistochemistry: Brg1 Antibody - BSA Free [NBP2-41270] - Immunohistochemistry of Brg1 in rat brain tissue with Brg1 antibody at 5 u/mL.
Immunohistochemistry: Brg1 Antibody - BSA Free [NBP2-41270] - Immunohistochemistry of Brg1 in rat brain tissue with Brg1 antibody at 5 u/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Brg1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 19 amino acid peptide near the carboxy terminus of human SMARCA4. The immunogen is located within amino acids 1420 - 1470 of SMARCA4. Amino Acid Squence: SRDKDDESKKQKKRGRPPAE
Specificity
Multiple isoforms of SMARCA4 are known to exist.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMARCA4
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 1-2 ug/ml
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-41270 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Brg1 Antibody - BSA Free

  • ATP-dependent helicase SMARCA4
  • BAF190
  • BAF190A
  • brahma protein-like 1
  • Brg1
  • BRG1-associated factor 190A
  • BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
  • BRM/SWI2-related gene 1
  • EC 3.6.1
  • EC 3.6.4.-
  • FLJ39786
  • global transcription activator homologous sequence
  • homeotic gene regulator
  • hSNF2b
  • Mitotic growth and transcription activator
  • nuclear protein GRB1
  • Protein brahma homolog 1
  • Protein BRG-1
  • RTPS2
  • SMARCA4
  • SNF2
  • SNF2B
  • SNF2-beta
  • SNF2L4
  • SNF2LB
  • SNF2-like 4
  • subfamily a, member 4
  • sucrose nonfermenting-like 4
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI2
  • transcription activator BRG1

Background

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Brg1 Antibody (NBP2-41270)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMARCA4