![Western Blot: Arylsulfatase D Antibody [NBP1-20886] - (0.3ug/ml) Staining of A459 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Arylsulfatase-D-Antibody-Western-Blot-NBP1-20886-img0001.jpg "Western Blot: Arylsulfatase D Antibody [NBP1-20886] - (0.3ug/ml) Staining of A459 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-DASNGYRALQWNA corresponding to internal region according to NP_001660.2, NP_033667.2. |
| Epitope | C terminus |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_001660.2; NP_033667.2) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | ARSD |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 60+40 kDa band bands observed in A549 and HepG2 lysates (calculated MW of 64.9 kDa band according to NP_001660.2 and of 42.3 according to NP_033667.2).
An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunizing peptide. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-20886 | Applications | Species |
|---|---|---|
| Lin YF, Yang J, Rosen BP. ArsD: an As(III) metallochaperone for the ArsAB As(III)-translocating ATPase. J Bioenerg Biomembr 2007-10-23 [PMID: 17955352] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Arylsulfatase D Antibody (NBP1-20886)Find related products by research area.
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| Gene Symbol | ARSD |
| Entrez |
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| Uniprot |
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