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Aminopeptidase N/CD13 Antibody (498001) [Unconjugated]

 (6 Publications)
 
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Western blot shows lysates of human kidney tissue and human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB3815) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Aminopeptidase N/CD13 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human kidney tissue and human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB3815) followed by ...read more
Aminopeptidase N/CD13 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB3815) at 8 µg/mL for 3 hours at room ...read more
Aminopeptidase N/CD13 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 25 µg/mL Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB3815) overnight at 4 ...read more
Aminopeptidase N/CD13 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB3815) at 1.7 µg/mL overnight at 4 ...read more
Simple Western lane view shows lysates of human small intestine tissue and human prostate tissue, loaded at 0.5 mg/mL. A specific band was detected for Aminopeptidase N/CD13 at approximately 204 kDa (as ...read more
Western blot shows lysates of U937 human histiocytic lymphoma cell line and human APN knockout U937 human histiocytic lymphoma cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Aminopeptidase ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF, KO
Clone
498001
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3815-SP
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Aminopeptidase N/CD13 Antibody (498001) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase N/CD13
Lys69-Lys967
Accession # AAA51719
Specificity
Detects human Aminopeptidase N/CD13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Aminopeptidase N is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ANPEP
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-25 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Knockout Validated 2 ug/mL
  • Simple Western 40 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB3815 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aminopeptidase N/CD13 Antibody (498001) [Unconjugated]

  • alanyl (membrane) aminopeptidase
  • Alanyl aminopeptidase
  • Aminopeptidase M
  • Aminopeptidase N
  • ANPEP
  • AP-M
  • APN
  • AP-N
  • CD13 antigen
  • CD13
  • CD13APN
  • EC 3.4.11
  • EC 3.4.11.2
  • gp150
  • LAP1
  • Microsomal aminopeptidase
  • Myeloid plasma membrane glycoprotein CD13
  • p150
  • PEPN
  • PEPNhAPN

Background

The human ANPEP gene encodes aminopeptidase N (APN), which is also known as microsomal aminopeptidase, alanyl aminopeptidase, aminopeptidase M, CD13, or membrane protein p161 (1‑3). The deduced amino acid sequence of human APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). The amino acid sequence of human APN is 78% and 77% identical to that of rat and mouse, respectively. Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (3).

  1. Olsen, J. et al. (1988) FEBS Lett. 238:307.
  2. Look, A.T. et al. (1989) J. Clin. Invest. 83:1299.
  3. Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 289, Academic Press, San Diego.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Aminopeptidase N/CD13 Antibody (498001) [Unconjugated]
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Publications for Aminopeptidase N/CD13 Antibody (MAB3815)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Co-Immunoprecipitation, ICC, IHC, IHC-P, Western Blot.


Filter By Application
Co-Immunoprecipitation
(1)
ICC
(1)
IHC
(1)
IHC-P
(2)
Western Blot
(1)
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Human
(5)
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Showing Publications 1 - 6 of 6.
Publications using MAB3815 Applications Species
Q Ma, Z Zhao, AP Sagare, Y Wu, M Wang, NC Owens, PB Verghese, J Herz, DM Holtzman, BV Zlokovic Blood-brain barrier-associated pericytes internalize and clear aggregated amyloid-?42 by LRP1-dependent apolipoprotein E isoform-specific mechanism Mol Neurodegener, 2018-10-19;13(1):57. 2018-10-19 [PMID: 30340601] (IHC-P, Human) IHC-P Human
EA Winkler, H Birk, JK Burkhardt, X Chen, JK Yue, D Guo, WC Rutledge, GF Lasker, C Partow, T Tihan, EF Chang, H Su, H Kim, BP Walcott, MT Lawton Reductions in brain pericytes are associated with arteriovenous malformation vascular instability J. Neurosurg., 2018-12-01;0(0):1-11. 2018-12-01 [PMID: 29303444] (IHC-P, Human) IHC-P Human
X Chen, Y Yuan, Q Wang, F Xie, D Xia, X Wang, Y Wei, T Xie Post-Translational Modification of Adiponectin Affects Lipid Accumulation, Proliferation and Migration of Vascular Smooth Muscle Cells Cell. Physiol. Biochem., 2017-08-28;43(1):172-181. 2017-08-28 [PMID: 28848139] (Western Blot, Human) Western Blot Human
S Å Urga, MP Nanut, J Kos, J Saboti? Fungal lectin MpL enables entry of protein drugs into cancer cells and their subcellular targeting Oncotarget, 2017-04-18;8(16):26896-26910. 2017-04-18 [PMID: 28460472] (Co-Immunoprecipitation, ICC, Human) Co-Immunoprecipitation, ICC Human
Jorgensen MM, Baek R, Varming K. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles 1/1/2015 [PMID: 25862471]
Emi Y, Harada Y, Sakaguchi M Involvement of a di-leucine motif in targeting of ABCC1 to the basolateral plasma membrane of polarized epithelial cells. Biochem Biophys Res Commun, 2013-10-12;441(1):89-95. 2013-10-12 [PMID: 24129190] (IHC, Human) IHC Human

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Bioinformatics

Gene Symbol ANPEP
Uniprot