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alcohol dehydrogenase Activity Assay Kit (Colorimetric)

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alcohol dehydrogenase Activity Assay Kit (Colorimetric) [NBP3-24463] - Standard Reference Curve
alcohol dehydrogenase Activity Assay Kit (Colorimetric) [NBP3-24463] - Assay Example

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Func
Suitable Sample Type
Serum, plasma and animal tissue
Standard Curve Range
0.29 - 248 U/L
Sensitivity
0.29 U/L

Order Details

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alcohol dehydrogenase Activity Assay Kit (Colorimetric) Summary

Description
Detection instrument: Microplate reader (440 - 460 nm, optimum wavelength: 450 nm)
Assay time: 30 min
Standard Curve Range
0.29 - 248 U/L
Sensitivity
0.29 U/L
Inter-Assay
%CV < 4.5
Intra-Assay
%CV < 4.2
Spike Recovery
Average recovery rate: 102%
Kit Type
Activity Assay Kit (Colorimetric)
Gene
ADH1A

Applications/Dilutions

Dilutions
  • Functional

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Microplate
  2. Plate Sealer
  3. Reagent 1: Substrate
  4. Reagent 2: Coenzyme
  5. Reagent 3: Coenzyme Diluent
  6. Reagent 4: Buffer Solution
  7. Reagent 5: Chromogenic Agent
  8. Reagent 6: Standard

Alternate Names for alcohol dehydrogenase Activity Assay Kit (Colorimetric)

  • ADH, alpha subunit
  • ADH1alcohol dehydrogenase 1A
  • alcohol dehydrogenase 1 (class I), alpha polypeptide
  • alcohol dehydrogenase 1A (class I), alpha polypeptide
  • Alcohol dehydrogenase subunit alpha
  • aldehyde reductase
  • EC 1.1.1
  • EC 1.1.1.1

Background

ADH (alcohol dehydrogenase) family of proteins metabolize a variety of substances such as ethanol, retinal, other aliphatic alcohol, hydroxysteroids, and lipid peroxidation products. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohol to ketones or aldehydes. ADH play a major role in ethanol metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ADH1A