alcohol dehydrogenase Activity Assay Kit (Colorimetric) Summary
Description |
Detection instrument: Microplate reader (440 - 460 nm, optimum wavelength: 450 nm) Assay time: 30 min |
Standard Curve Range |
0.29 - 248 U/L |
Sensitivity |
0.29 U/L |
Inter-Assay |
%CV < 4.5 |
Intra-Assay |
%CV < 4.2 |
Spike Recovery |
Average recovery rate: 102% |
Kit Type |
Activity Assay Kit (Colorimetric) |
Gene |
ADH1A |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Microplate
- Plate Sealer
- Reagent 1: Substrate
- Reagent 2: Coenzyme
- Reagent 3: Coenzyme Diluent
- Reagent 4: Buffer Solution
- Reagent 5: Chromogenic Agent
- Reagent 6: Standard
|
Alternate Names for alcohol dehydrogenase Activity Assay Kit (Colorimetric)
Background
ADH (alcohol dehydrogenase) family of proteins metabolize a variety of substances such as ethanol, retinal, other aliphatic alcohol, hydroxysteroids, and lipid peroxidation products. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohol to ketones or aldehydes. ADH play a major role in ethanol metabolism.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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