alcohol dehydrogenase 5 Antibody (3E7)

Images

 
Western Blot: alcohol dehydrogenase 5 Antibody (3E7) [NBP3-42978] - fetal human brain tissue were subjected to SDS PAGE followed by western blot with (alcohol dehydrogenase 5 Antibody) at dilution of 1:4000
Immunohistochemistry: alcohol dehydrogenase 5 Antibody (3E7) [NBP3-42978] - Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using (alcohol dehydrogenase 5 Antibody) at dilution of 1:50
Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 5 Antibody (3E7) [NBP3-42978] - Immunofluorescent analysis of HepG2 cells using (alcohol dehydrogenase 5 antibody) at dilution of 1:50 and Rhodamine-Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
3E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

alcohol dehydrogenase 5 Antibody (3E7) Summary

Immunogen
alcohol dehydrogenase 5(class III), chi polypeptide - (Uniprot# P11766)
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ADH5
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20 - 1:200
  • Immunohistochemistry 1:20 - 1:200
  • Western Blot 1:1000 - 1:8000
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A or G purified

Alternate Names for alcohol dehydrogenase 5 Antibody (3E7)

  • ADH-3
  • ADHXEC 1.1.1.1
  • alcohol dehydrogenase (class III), chi polypeptide
  • alcohol dehydrogenase 5 (class III), chi polypeptide
  • Alcohol dehydrogenase 5
  • Alcohol dehydrogenase class chi chain
  • alcohol dehydrogenase class-3
  • Alcohol dehydrogenase class-III
  • EC 1.1.1
  • EC 1.1.1.-
  • EC 1.1.1.284
  • FALDH
  • FDHGSNOR
  • formaldehyde dehydrogenase
  • Glutathione-dependent formaldehyde dehydrogenase
  • GSH-FDH
  • S-(hydroxymethyl)glutathione dehydrogenase

Background

The ADH5 gene encodes glutathione dependent formaldehyde dehydrogenase or class III alcohol dehydrogenase chi subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, retinol, ethanol and other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class III alcohol dehydrogenase is a homodimer composed of 2 chi subunits. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long chain primary alcohols and for oxidation of S hydroxymethyl glutathione, a spontaneous adduct between formaldehyde and glutathione. The enzyme is an important component of cellular metabolism for the elimination of formaldehyde, which is a potent irritant and sensitizing agent that causes rhinitis, lacrymation, contact dermatitis and pharyngitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ADH5