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AG-3/AGR3 Antibody

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Western blot shows lysates of human small intestine tissue. PVDF Membrane was probed with 0.5 µg/mL of Human AG-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6307) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AG-3 at approximately 19kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human small intestine tissue. PVDF Membrane was probed with 0.5 µg/mL of Human AG-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6307) followed by HRP-conjugated Anti-Sheep ...read more
AG-3 was detected in immersion fixed paraffin-embedded sections of human breast using Sheep Anti-Human AG-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6307) at 3 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF6307-SP
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AG-3/AGR3 Antibody Summary

Immunogen
E.coli-derived Recombinant Human AG-3
Ile22-Leu166
Accession # Q8TD06
Specificity
Detects human AG-3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human AG‑2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
AGR3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AG-3/AGR3 Antibody

  • AG3
  • AG-3
  • AGR3
  • anterior gradient homolog 3 (Xenopus laevis)
  • BCMP11
  • BCMP11AG3
  • Breast cancer membrane protein 11
  • hAG-3member 18
  • PDIA18

Background

AG3 (Anterior gradient protein 3; also known as AGR3 and BCMP11) is a 17 kDa (predicted) member of the AGR family of proteins. It is expressed by intestinal epithelial and breast cancer cells. AG3 is presumably secreted and binds to both a-dystroglycan and C4.4a/LYPD3. Mature human AG3 is 145 amino acids (aa) in length (aa 22‑166) (SwissProt #:Q8TD06). Over aa 22-166, human AG3 shares 92% amino acid sequence identity with mouse AG3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AGR3
Uniprot