Goat Adiponectin/Acrp30 ELISA Kit (Colorimetric) Summary
Specificity |
This assay has high sensitivity and excellent specificity for detection of Goat Adiponectin/Acrp30. No significant cross-reactivity or interference between Goat Adiponectin/Acrp30 and analogues was observed. |
Standard Curve Range |
0.312 ng/mL - 20 ng/mL |
Sensitivity |
0.078 ng/mL |
Assay Type |
Competitive ELISA |
Inter-Assay |
%CV < 10 |
Intra-Assay |
%CV < 8 |
Spike Recovery |
86 - 97% |
Sample Volume |
50 - 100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
ADIPOQ |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
Components
|
- Adhesive Strip (For 96 wells)
- Assay Plate
- HRP-Conjugate (100 x concentrate)
- HRP-Conjugate Diluent
- Instruction Manual
- Sample Diluent
- Standard (Freeze dried)
- Stop Solution
- TMB Substrate
- Wash Buffer (25 x concentrate)
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Alternate Names for Goat Adiponectin/Acrp30 ELISA Kit (Colorimetric)
Background
Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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