![Western Blot: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adiponectin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Adiponecti](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-OTI1H1-Azide-and-BSA-Free-Western-Blot-NBP2-71422-img0001.jpg "Western Blot: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adiponectin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Adiponecti")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI1H1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 19-244 of human ADIPOQ(NP_004788) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ADIPOQ |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 24.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
Research Areas for Adiponectin/Acrp30 Antibody (NBP2-71422)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ADIPOQ |
![Immunocytochemistry/Immunofluorescence: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Adiponectin.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-OTI1H1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71422-img0003.jpg "Immunocytochemistry/Immunofluorescence: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Adiponectin.")
![Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Human Kidney tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-OTI1H1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71422-img0008.jpg "Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Human Kidney tissue using anti-Adiponectin mouse monoclonal antibody.")
![Flow Cytometry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Adiponectin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-OTI1H1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71422-img0002.jpg "Flow Cytometry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Adiponectin antibody, and then analyzed by flow cytometry.")
![Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-OTI1H1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71422-img0004.jpg "Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Adiponectin mouse monoclonal antibody.")
![Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-OTI1H1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71422-img0005.jpg "Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Adiponectin mouse monoclonal antibody.")
![Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-OTI1H1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71422-img0006.jpg "Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Adiponectin mouse monoclonal antibody.")
![Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Human breast tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-OTI1H1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71422-img0007.jpg "Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Human breast tissue using anti-Adiponectin mouse monoclonal antibody.")
![Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Human liver tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-OTI1H1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71422-img0009.jpg "Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Human liver tissue using anti-Adiponectin mouse monoclonal antibody.")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![N/A Leptin/OB [HRP]](https://images.novusbio.com/images2/Leptin_DLP00_ELISA_134.jpg)
![N/A Leptin/OB [HRP]](https://images.novusbio.com/images2/DATA_Leptin_DLP00_ELISA_749.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![N/A C-Reactive Protein/CRP [Biotin]](https://images.novusbio.com/images2/DATA_CReactive_Protein_DY1707_ELISA_1701.jpg)
![N/A Resistin [HRP]](https://images.novusbio.com/images2/Resistin_DRSN00_ELISA_174.jpg)
![N/A Resistin [HRP]](https://images.novusbio.com/images2/DATA_Resistin_DRSN00_ELISA_814.jpg)
![N/A CCL2/JE/MCP-1 [HRP]](https://images.novusbio.com/images2/CCL2_DCP00_ELISA_67.jpg)
![N/A CCL2/JE/MCP-1 [HRP]](https://images.novusbio.com/images2/DATA_CCL2_DCP00_ELISA_633.jpg)
![N/A CCL2/JE/MCP-1 [HRP]](https://images.novusbio.com/images2/DATA_CCL2_DCP00_ELISA_634.jpg)
![Western Blot AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Western_Blot_5219.jpg)
![Simple Western AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Simple_Western_16678.jpg)
![Immunocytochemistry AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Immunocytochemistry__Immunofluorescence_19686.jpg)
![Western Blot AMPK beta 1 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_beta_1_AF2854_Western_Blot_5220.jpg)
![Immunohistochemistry AMPK beta 1 Antibody [Unconjugated]](https://images.novusbio.com/images/af2854_human-mouse-rat-ampk-beta-1-affinity-purified-polyclonal-ab-immunohistochemistry-252023124938.jpg)
![Immunohistochemistry AMPK beta 1 Antibody [Unconjugated]](https://images.novusbio.com/images/af2854_human-mouse-rat-ampk-beta-1-affinity-purified-polyclonal-ab-immunohistochemistry-252023125022.jpg)
![SDS-PAGE IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_40.jpg)
![Bioactivity IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_41.jpg)
![Mass Spectrometry IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_42.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")