![Western Blot: ACOT1 Antibody [NBP3-12713] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/ACOT1-Antibody-Western-Blot-NBP3-12713-img0001.jpg "Western Blot: ACOT1 Antibody [NBP3-12713] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa(We are unsure as to the identity of these extra bands.)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. Protein A & Antigen Affinity purified |
| Immunogen | Produced in rabbits immunized with E. coli-derived Human ACOT1 fragment |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ACOT1 |
| Purity | Antigen and protein A Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.03% Proclin 300 |
| Purity | Antigen and protein A Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ACOT1 |
![Immunocytochemistry/Immunofluorescence: ACOT1 Antibody [NBP3-12713] - Staining of ACOT1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ACOT1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/ACOT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12713-img0004.jpg "Immunocytochemistry/Immunofluorescence: ACOT1 Antibody [NBP3-12713] - Staining of ACOT1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ACOT1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: ACOT1 Antibody [NBP3-12713] - Staining of human ACOT1 in human kidney with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ACOT1-Antibody-Immunohistochemistry-Paraffin-NBP3-12713-img0003.jpg "Immunohistochemistry-Paraffin: ACOT1 Antibody [NBP3-12713] - Staining of human ACOT1 in human kidney with rabbit polyclonal antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: ACOT1 Antibody [NBP3-12713] - Staining of human ACOT1 in human brain with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ACOT1-Antibody-Immunohistochemistry-Paraffin-NBP3-12713-img0002.jpg "Immunohistochemistry-Paraffin: ACOT1 Antibody [NBP3-12713] - Staining of human ACOT1 in human brain with rabbit polyclonal antibody at 1:500 dilution.")
![Immunoprecipitation: ACOT1 Antibody [NBP3-12713] - Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-ACOT1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ACOT1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size :46 kDa](http://images.novusbio.com/fullsize/ACOT1-Antibody-Immunoprecipitation-NBP3-12713-img0005.jpg "Immunoprecipitation: ACOT1 Antibody [NBP3-12713] - Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-ACOT1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ACOT1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size :46 kDa")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")