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Rat Acetylcholinesterase/ACHE ELISA Kit (Chemiluminescence)

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ELISA: Rat Acetylcholinesterase/ACHE ELISA Kit (Chemiluminescence) [NBP2-66391] - Samples were spiked with high concentrations of Rat Acetylcholinesterase/ACHE and diluted with Reference Standard & Sample Diluent to ...read more
ELISA: Rat Acetylcholinesterase/ACHE ELISA Kit (Chemiluminescence) [NBP2-66391] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16-10 ng/mL
Sensitivity
0.09 ng/mL

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Rat Acetylcholinesterase/ACHE ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Rat AChE in samples. No significant cross-reactivity or interference between Rat AChE and analogues was observed.
Standard Curve Range
0.16-10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 10.23%
Intra-Assay
CV% < 8.89%
Spike Recovery
88-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
ACHE

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Rat Acetylcholinesterase/ACHE ELISA Kit (Chemiluminescence)

  • acetylcholinesterase (Yt blood group)
  • Acetylcholinesterase
  • ACHE
  • apoptosis-related acetylcholinesterase
  • ARACHE
  • EC 3.1.1
  • EC 3.1.1.7
  • N-ACHE
  • Yt blood group
  • YT

Background

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ACHE