![Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Paraffin-embedded rat brain. 5-Methylcytosine / 5-mC antibody diluted at 1:200.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Paraffin-NBP2-42814-img0008.jpg "Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Paraffin-embedded rat brain. 5-Methylcytosine / 5-mC antibody diluted at 1:200.")
| Reactivity | AllSpecies Glossary |
| Applications | DB, IHC, IP, MS, IHC |
| Clone | 4111 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | The immunogen used to generate this antibody corresponds to 5-MethylCytosine |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of human ovarian carcinoma. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:200.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Paraffin-NBP2-42814-img0002.jpg "Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of human ovarian carcinoma. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:200.")
![Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of mouse testis. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:200.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Paraffin-NBP2-42814-img0003.jpg "Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of mouse testis. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:200.")
![Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of mouse urinary bladder. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:200.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Paraffin-NBP2-42814-img0004.jpg "Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of mouse urinary bladder. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:200.")
![Immunohistochemistry Whole-Mount: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:50.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Whole-Mount-NBP2-42814-img0005.jpg "Immunohistochemistry Whole-Mount: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:50.")
![Methylated DNA Immunoprecipitation: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:50.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Methylated-DNA-Immunoprecipitation-NBP2-42814-img0006.jpg "Methylated DNA Immunoprecipitation: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:50.")
![Dot Blot: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of anti-5-mC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine .](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Dot-Blot-NBP2-42814-img0001.jpg "Dot Blot: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of anti-5-mC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine .")
![Methylated DNA Immunoprecipitation: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of human genomic DNA (500 ng) mixed with methylated cytosine standard kit, using 5-Methylcytosine/ 5-mC antibody at 1:500 dilution. Analysized with semi-quantitative PCR and Immunprecipitated DNA was plotted as % of input DNA.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Methylated-DNA-Immunoprecipitation-NBP2-42814-img0007.jpg "Methylated DNA Immunoprecipitation: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of human genomic DNA (500 ng) mixed with methylated cytosine standard kit, using 5-Methylcytosine/ 5-mC antibody at 1:500 dilution. Analysized with semi-quantitative PCR and Immunprecipitated DNA was plotted as % of input DNA.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")