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5-HT3A Antibody (4D5E7) - BSA Free

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Western Blot: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis using HTR3A mAb against human HTR3A (AA: extra 24-157) recombinant protein. (Expected MW is 41.9 kDa)
Immunocytochemistry/ Immunofluorescence: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis of Hela cells using HTR3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Immunohistochemistry-Paraffin: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis of cervical cancer tissues using HTR3A mouse mAb with DAB staining.
Flow Cytometry: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis of Hela cells using HTR3A mouse mAb (green) and negative control (red).
Western Blot: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Analysis using HTR3A mAb against HEK293 (1) and HTR3A (AA: extra 24-157)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: 5-HT3A Antibody (4D5E7) [NBP2-61786] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
4D5E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Catalog# & Formulation Size Price
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5-HT3A Antibody (4D5E7) - BSA Free Summary

Immunogen
Purified recombinant fragment of human 5-HT3A (AA: extra 24-157) expressed in E. Coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HTR3A
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Theoretical MW
55.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for 5-HT3A Antibody (4D5E7) - BSA Free

  • 5-HT-3,5-HT3-A
  • 5HT3A
  • 5-HT3A
  • 5-HT3R5-hydroxytryptamine receptor 3A
  • 5-hydroxytryptamine (serotonin) receptor 3A
  • 5-hydroxytryptamine receptor 3,5-HT3A5HT3R
  • HTR35HT3 serotonin receptor
  • HTR3A
  • Serotonin receptor 3A
  • Serotonin-gated ion channel receptor

Background

The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HTR3A