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14-3-3 epsilon Antibody (02) - Azide and BSA Free

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Western Blot: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] - NBP3-26078 at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: U-251 MG Whole Cell Lysate. Lane D: A375 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa
Western Blot: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] - NBP3-26078 at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: U-251 MG Whole Cell Lysate. Lane D: ...read more
Immunoprecipitation: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] - 14-3-3 epsilon was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. Lane C: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clone
02
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-26078AF350
Alexa Fluor 405
NBP3-26078AF405
Alexa Fluor 488
NBP3-26078AF488
Alexa Fluor 532
NBP3-26078AF532
Alexa Fluor 594
NBP3-26078AF594
Alexa Fluor 647
NBP3-26078AF647
Alexa Fluor 700
NBP3-26078AF700
Alexa Fluor 750
NBP3-26078AF750
Allophycocyanin
NBP3-26078APC
Biotin
NBP3-26078B
CoraFluor 1
NBP3-26078CL1
DyLight 350
NBP3-26078UV
DyLight 405
NBP3-26078V
DyLight 488
NBP3-26078G
DyLight 550
NBP3-26078R
DyLight 594
NBP3-26078DL594
DyLight 650
NBP3-26078C
DyLight 680
NBP3-26078FR
DyLight 755
NBP3-26078IR
FITC
NBP3-26078F
HRP
NBP3-26078H
Janelia Fluor 525
NBP3-26078JF525
Janelia Fluor 549
NBP3-26078JF549
Janelia Fluor 585
NBP3-26078JF585
Janelia Fluor 635
NBP3-26078JF635
Janelia Fluor 646
NBP3-26078JF646
Janelia Fluor 669
NBP3-26078JF669
PE
NBP3-26078PE
PerCP
NBP3-26078PCP
mFluor Violet 450 SE
NBP3-26078MFV450
mFluor Violet 500 SE
NBP3-26078MFV500
mFluor Violet 610 SE
NBP3-26078MFV610
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14-3-3 epsilon Antibody (02) - Azide and BSA Free Summary

Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 epsilon (Accession#: NP_006752.1; Met1-Gln255).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
YWHAE
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 1-5 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for 14-3-3 epsilon Antibody (02) - Azide and BSA Free

  • 1433 epsilon
  • 14-3-3 epsilon
  • 14-3-3 protein epsilon
  • FLJ45465
  • FLJ53559,14-3-3E
  • KCIP-1
  • MDCR
  • MDS
  • mitochondrial import stimulation factor L subunit
  • protein kinase C inhibitor protein-1
  • tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilonpolypeptide
  • YWHAE

Background

14-3-3 epsilon product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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14-3-3 epsilon
Antibody (02) - Azide ...
NBP3-26078
14-3-3 epsilon Antibody (02) - Azide and BSA Free
Species: Hu
Applications: WB, IP

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Bioinformatics

Gene Symbol YWHAE