Choose from nearly 1,000 secondary antibodies conjugated to more than 40 different enzymatic, fluorescent, or biotin labels. Use the filters on the left to sort our secondary antibodies by species, application, host, or conjugate.
To learn more about secondary antibodies, download and read our Secondary Antibody Handbook for technical information and tips to guide you when choosing a secondary antibody for your experiment.
To understand more about direct vs. indirect detection, including benefits of a secondary antibody and when to use one, please read our resources on Direct vs. Indirect Detection Methods.
![Western Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Golden Syrian Hamster IgG (Lane 2) and Armenian Hamster IgG (Lane 3) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 5% Blotto 30 min at 20C, Antibody was used at 1:5000 in blocking buffer for Fluorescent Western blotting. Molecular weight markers are in lane 1.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-71730-img0003.jpg "Western Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Golden Syrian Hamster IgG (Lane 2) and Armenian Hamster IgG (Lane 3) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 5% Blotto 30 min at 20C, Antibody was used at 1:5000 in blocking buffer for Fluorescent Western blotting. Molecular weight markers are in lane 1.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - This image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).. Using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-71730-img0006.jpg)
![Western Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Anti-TNF-alpha was detected using a DyLight 800 conjugate. The image was captured using the Odyssey Infrared Imaging System developed by LI-COR.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-71730-img0002.jpg)
![ELISA: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - ELISA results of purified Goat anti-Hamster IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed) tested against purified Golden Syrian Hamster IgG and Armenian Hamster IgG. Each well was coated in duplicate with 1.0 ug of protein from different species. Golden Syrian Hamster IgG (Green Line), Armenian Hamster IgG (Red Line), Mouse IgG (Blue Line), and Rat IgG (Purple Line). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-71730-img0004.jpg)
![Dot Blot: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Dot Blot for Goat anti-Hamster IgG (H+L) Secondary Antibody [Texas Red] (Pre-adsorbed). Lane 1: 100 ng Hamster IgG. Lanes 2-5: serial dilution 3 fold of Antigen. Primary Antibody: none.Secondary Antibody: Goat anti-Hamster IgG (H+L) Secondary Antibody [Texas Red] (Pre-adsorbed) at 1:1000 dilution at RT for 1 hour. Block: Fluorescent blocking buffer at RT for 30 minutes.. Using the Texas Red format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NBP1-71730-img0005.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-71730] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Hamster-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP1-71730-img0008.jpg)