Gigaxonin Antibodies
We offer Gigaxonin Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunoprecipitation, Sandwich ELISA, Western Blot. Each Gigaxonin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Gigaxonin Antibodies can be used in a variety of model species: Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat. Use the list below to choose the Gigaxonin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Gigaxonin polyclonal antibodies and browse our Gigaxonin monoclonal antibody catalog.
Alternate Names for Gigaxonin Antibodies
anti-Gigaxonin antibody, anti-GAN antibody, anti-FLJ38059 antibody, anti-GAN1KLHL16Kelch-like protein 16 antibody, anti-giant axonal neuropathy (gigaxonin) antibody
6 Results for "gigaxonin" in Primary Antibodies
![Western Blot: Gigaxonin Antibody [NBP1-49924] - The Kelch domain of GIG interacts with intermediate filament proteins. Endogenous VIM pulls down endogenous GIG. Indicated lysates were subjected to immunoprecipitation with either VIM antibody or control IgG (used as the mock condition throughout), and the membrane was probed sequentially for endogenous GIG and then VIM (not shown). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140157), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Gigaxonin-Antibody-Western-Blot-NBP1-49924-img0002.jpg "Western Blot: Gigaxonin Antibody [NBP1-49924] - The Kelch domain of GIG interacts with intermediate filament proteins. Endogenous VIM pulls down endogenous GIG. Indicated lysates were subjected to immunoprecipitation with either VIM antibody or control IgG (used as the mock condition throughout), and the membrane was probed sequentially for endogenous GIG and then VIM (not shown). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140157), licensed under a CC-BY license.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Gigaxonin Antibody [NBP1-49924] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Gigaxonin antibody NBP1-49924 used for IP at 3 ug/mg lysate. Gigaxonin was also immunoprecipitated by rabbit anti-Gigaxonin antibody NBP1-49923, which recognizes an upstream epitope. For blotting immunoprecipitated Gigaxonin, NBP1-49924 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Gigaxonin-Antibody-Immunoprecipitation-NBP1-49924-img0001.jpg)
![Immunoprecipitation: Gigaxonin Antibody [NBP1-49924] - The Kelch domain of GIG interacts with intermediate filament proteins. GIG pulls down overexpressed PRPH in a Kelch-dependent manner. Cell lysates were subjected to immunoprecipitation with anti-Flag beads or IgG, and the membrane was probed sequentially for PRPH then GIG (not shown). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140157), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Gigaxonin-Antibody-Immunoprecipitation-NBP1-49924-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Gigaxonin Antibody [NBP1-49924] - Transfection with wild type Gigaxonin. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-49924_rabbit-polyclonal-gigaxonin-antibody-immunocytochemistry-immunofluorescence-196202316639..jpg)
![Western Blot: Gigaxonin Antibody [NBP1-49924] - Design of proteomic bait to determine the binding partners of GIG.(A) GIG is a BTB/Kelch protein, & these domains are separated by an intervening BACK domain. GIG is thought to dimerize through its BTB domain [16]. The BTB domain has also been shown to bind to E3 ligases, potentially for ubiquitin tagging of Kelch targets. To identify potential substrates for degradation/modification (Kelch binding partners) we expressed flag-tagged full-length GIG (B) or its BTB domain (C). (D) Sequence of proteomic baits with key regions identified by color. The constructs expressed included a single flag tag & linker (grey), Biotag (black), BTB domain (blue), BACK domain (red), & Kelch domains (green). The location of the truncation for the BTB bait shown in (C) is indicated by the black line at amino acid 268, towards the N-terminus of the BACK domain. (E) Flag-tagged constructs were expressed in HEK293 cells & affinity purified with an anti-Flag antibody (abbreviated as F). The membrane was then probed with anti-Flag tag antibodies. Untransfected cells (NC) were included to control for non-specific binding to the antibody or beads. Flag-tagged GIG was ~62 kDa, while the BTB domain was ~34 kDa & forms a dimer in the immunoprecipitation sample that was ~70 kDa. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26460568), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-49924_rabbit-polyclonal-gigaxonin-antibody-310202416163788.jpg)
![Immunocytochemistry/Immunofluorescence: Gigaxonin Antibody [NBP2-33569] - Immunofluorescent staining of human cell line RT4 shows localization to microtubules.](http://images.novusbio.com/fullsize/Gigaxonin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33569-img0002.jpg "Immunocytochemistry/Immunofluorescence: Gigaxonin Antibody [NBP2-33569] - Immunofluorescent staining of human cell line RT4 shows localization to microtubules.")
![Immunocytochemistry/Immunofluorescence: Gigaxonin Antibody (4G7) [H00008139-M01] - Analysis of monoclonal antibody to GAN on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Gigaxonin-Antibody-4G7-Immunocytochemistry-Immunofluorescence-H00008139-M01-img0004.jpg "Immunocytochemistry/Immunofluorescence: Gigaxonin Antibody (4G7) [H00008139-M01] - Analysis of monoclonal antibody to GAN on HeLa cell . Antibody concentration 10 ug/ml.")
![Sandwich ELISA: Gigaxonin Antibody (4G7) [H00008139-M01] - Detection limit for recombinant GST tagged GAN is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Gigaxonin-Antibody-4G7-Sandwich-ELISA-H00008139-M01-img0005.jpg)
![Immunoprecipitation: Gigaxonin Antibody [NBP1-49923] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Gigaxonin antibody NBP1-49923 used for IP at 3 ug/mg lysate. Gigaxonin was also immunoprecipitated by rabbit anti-Gigaxonin antibody NBP1-49924, which recognizes a downstream epitope. For blotting immunoprecipitated Gigaxonin, NBP1-49924 was used at 1ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Gigaxonin-Antibody-Immunoprecipitation-NBP1-49923-img0001.jpg "Immunoprecipitation: Gigaxonin Antibody [NBP1-49923] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Gigaxonin antibody NBP1-49923 used for IP at 3 ug/mg lysate. Gigaxonin was also immunoprecipitated by rabbit anti-Gigaxonin antibody NBP1-49924, which recognizes a downstream epitope. For blotting immunoprecipitated Gigaxonin, NBP1-49924 was used at 1ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: Gigaxonin Antibody [NBP1-57821] - THP-1 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Gigaxonin-Antibody-Western-Blot-NBP1-57821-img0002.jpg "Western Blot: Gigaxonin Antibody [NBP1-57821] - THP-1 cell lysate, concentration 0.2-1 ug/ml.")