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DOK7 Antibodies

We offer DOK7 Antibodies for use in common research applications: Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot. Each DOK7 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our DOK7 Antibodies can be used in a variety of model species: Human, Monkey, Mouse, Rat. Use the list below to choose the DOK7 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our DOK7 polyclonal antibodies and browse our DOK7 monoclonal antibody catalog.

Alternate Names for DOK7 Antibodies

anti-DOK7 antibody, anti-C4orf25chromosome 4 open reading frame 25 antibody, anti-CMS1B antibody, anti-docking protein 7 antibody, anti-Dok-7 antibody, anti-Downstream of tyrosine kinase 7 antibody, anti-FLJ33718 antibody, anti-FLJ39137 antibody, anti-FLJ90556 antibody, anti-protein Dok-7 antibody

6 Results for "dok7" in Primary Antibodies

NBP2-02073

DOK7 Antibody (OTI1A9)

(2 Publications)

0.1 ml

NBP2-02073

Western Blot: DOK7 Antibody (1A9) [NBP2-02073] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-DOK7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: DOK7 Antibody (1A9) [NBP2-02073] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK7.

Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Human prostate tissue using anti-DOK7 mouse monoclonal antibody.

Flow Cytometry: DOK7 Antibody (1A9) [NBP2-02073] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DOK7 antibody, and then analyzed by flow cytometry.

Western Blot: DOK7 Antibody (1A9) [NBP2-02073] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK7.

Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DOK7 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DOK7 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Human liver tissue using anti-DOK7 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: DOK7 Antibody (1A9) [NBP2-02073] - Staining of paraffin-embedded Human pancreas tissue using anti-DOK7 mouse monoclonal antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-72494AF350
Alexa Fluor 405	NBP2-72494AF405
Alexa Fluor 488	NBP2-72494AF488
Alexa Fluor 532	NBP2-72494AF532
Alexa Fluor 594	NBP2-72494AF594
Alexa Fluor 647	NBP2-72494AF647
Alexa Fluor 700	NBP2-72494AF700
Alexa Fluor 750	NBP2-72494AF750
Allophycocyanin	NBP2-72494APC
Allophycocyanin/Cy7	NBP2-72494APCCY7
Biotin	NBP2-72494B
CoraFluor 1	NBP2-72494CL1
DyLight 350	NBP2-72494UV
DyLight 405	NBP2-72494V
DyLight 488	NBP2-72494G
DyLight 550	NBP2-72494R
DyLight 594	NBP2-72494DL594
DyLight 650	NBP2-72494C
DyLight 680	NBP2-72494FR
DyLight 755	NBP2-72494IR
FITC	NBP2-72494F
HRP	NBP2-72494H
Janelia Fluor 525	NBP2-72494JF525
Janelia Fluor 549	NBP2-72494JF549
Janelia Fluor 585	NBP2-72494JF585
Janelia Fluor 635	NBP2-72494JF635
Janelia Fluor 646	NBP2-72494JF646
Janelia Fluor 669	NBP2-72494JF669
PE	NBP2-72494PE
PE/Atto594	NBP2-72494PEATT594
PE/Cy5.5	NBP2-72494PECY55
PE/Cy7	NBP2-72494PECY7
PerCP	NBP2-72494PCP
mFluor Violet 450 SE	NBP2-72494MFV450
mFluor Violet 500 SE	NBP2-72494MFV500
mFluor Violet 610 SE	NBP2-72494MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-72494

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AF6398

DOK7 Antibody [Unconjugated]

(3 Publications)

100 μg

AF6398

Western blot shows lysates of untreated MCF-7 human breast cancer cell line and T47D human breast cancer cell line and C2C12 mouse myoblast cell line and L6 rat myoblast cell line untreated (-) or treated (+) with 2% horse serum for 6 days. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat DOK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DOK7 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for DOK7 at approximately 60 & 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat DOK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Sorbs1 is enriched at AChR aggregates, and Sorbs1 RNAi blocks AChR clustering in vitro. (A) Treatment of myotubes with siRNA directed against Sorbs1 blocks AChR clustering in C2C12 cells. Montages containing sixteen fields at a magnification of 10× were analyzed with ImageJ software (NIH). (B) Sorbs1 siRNA significantly reduces Sorbs1 protein expression in myotubes. (C) Sorbs1 protein is highly enriched at sites where AChRs aggregate. (D) Agrin stimulates tyrosine phosphorylation of MuSK and Dok-7 at similar levels in myotubes treated with Sorbs1 siRNA. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26527617), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6398-SP

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NBP2-32465

DOK7 Antibody

0.1 ml

NBP2-32465

Western Blot: DOK7 Antibody [NBP2-32465] - Analysis in human cell line MCF-7 and human cell line SK-MEL-30.

Immunocytochemistry/Immunofluorescence: DOK7 Antibody [NBP2-32465] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: DOK7 Antibody [NBP2-32465] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

<b>Independent Antibodies Validation. </b>Western Blot: DOK7 Antibody [NBP2-32465] - Analysis NBP2-32465 (A) shows similar pattern to independent antibody NBP2-68794 (B).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB6398

DOK7 Antibody (651601)

100 μg

MAB6398

DOK7 was detected in immersion fixed C2C12 mouse myoblast cell line differentiated into myotubes using Mouse Anti-Human DOK7 Monoclonal Antibody (Catalog # MAB6398) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6398-SP

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NBP2-68794

DOK7 Antibody

100 ul

NBP2-68794

Western Blot: DOK7 Antibody [NBP2-68794] - Analysis in human cell line MCF-7 and human cell line SK-MEL-30.

Immunocytochemistry/Immunofluorescence: DOK7 Antibody [NBP2-68794] - Staining of human cell line MCF7 shows localization to nucleus.

<b>Independent Antibodies Validation. </b>Western Blot: DOK7 Antibody [NBP2-68794] - Analysis NBP2-68794 (A) shows similar pattern to independent antibody NBP2-32465 (B).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-46472

DOK7 Antibody (OTI2C12)

0.1 ml

NBP2-46472

Western Blot: DOK7 Antibody (2C12) [NBP2-46472] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK7.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse monoclonal

Host: Mouse monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-72491AF350
Alexa Fluor 405	NBP2-72491AF405
Alexa Fluor 488	NBP2-72491AF488
Alexa Fluor 532	NBP2-72491AF532
Alexa Fluor 594	NBP2-72491AF594
Alexa Fluor 647	NBP2-72491AF647
Alexa Fluor 700	NBP2-72491AF700
Alexa Fluor 750	NBP2-72491AF750
Allophycocyanin	NBP2-72491APC
Biotin	NBP2-72491B
CoraFluor 1	NBP2-72491CL1
DyLight 350	NBP2-72491UV
DyLight 405	NBP2-72491V
DyLight 488	NBP2-72491G
DyLight 550	NBP2-72491R
DyLight 594	NBP2-72491DL594
DyLight 650	NBP2-72491C
DyLight 680	NBP2-72491FR
DyLight 755	NBP2-72491IR
FITC	NBP2-72491F
HRP	NBP2-72491H
Janelia Fluor 525	NBP2-72491JF525
Janelia Fluor 585	NBP2-72491JF585
Janelia Fluor 635	NBP2-72491JF635
Janelia Fluor 669	NBP2-72491JF669
PE	NBP2-72491PE
PerCP	NBP2-72491PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-72491

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