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Response To Water Deprivation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Response To Water Deprivation Pathway and Deprivation Of Water, Dehydration, Diabetes Mellitus, Disease Of Adrenal Medulla, Polydipsia. The study of the Response To Water Deprivation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Response To Water Deprivation Pathway has been researched in relation to Excretion, Secretion, Transport, Glomerular Filtration, Aging. The Response To Water Deprivation Pathway complements our catalog of research reagents including antibodies and ELISA kits against NEPHROGENIC DIABETES INSIPIDUS, AQUAPORIN 2, AQUAPORIN 1, AQUAPORIN 3, CYCLOOXYGENASE 2.

Top Research Reagents

We have 1591 products for the study of the Response To Water Deprivation Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

Aquaporin-2 Antibody - BSA Free
NB110-74682
Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

32 Publications
Serpin A8/Angiotensinogen Antibody - BSA Free
NBP1-30027
Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Angiotensinogen at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

11 Publications
COX-1 Antibody
NBP1-85500
Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining in human skin and skeletal muscle tissues using anti-PTGS1 antibody. Corresponding PTGS1 RNA-seq data are presented for the same tissues.Western Blot: COX-1 Antibody [NBP1-85500] - Analysis in human cell line HELA.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Simple Western, ICC/IF

     1 Review

3 Publications
COX-2 Antibody
NB100-689
Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody (NB100-689).Simple Western: COX-2 Antibody [NB100-689] - Simple Western lane view shows a specific band for COX2 in 1.0 mg/ml of Rat Brain at an antibody concentration of 1:100. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image provided through customer review.

Rabbit Polyclonal
Species Human, Rat
Applications WB, Simple Western, IHC

     1 Review

36 Publications
Aquaporin-3 Antibody
NBP1-97927
Western Blot: Aquaporin-3 Antibody [NBP1-97927] - Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin-3 protein using Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927). Primary Antibody: Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927) at 1:2000.Immunohistochemistry: Aquaporin-3 Antibody [NBP1-97927] - Immunohistochemistry analysis using Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 m cryostat sections.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

7 Publications
Glut1 Antibody - BSA Free
NB110-39113
Western Blot: Glut1 Antibody [NB110-39113] - PrP-mediated increase in IC iron downregulates glucose transporters in the brain, neuroretina, and the liver: A similar evaluation of retinal lysates shows upregulation of Glut1 in PrP-/- relative to Tg40 PrP samples (lanes 1 & 3). Iron overloading downregulates Glut1 in Tg40 PrP samples relative to untreated controls, but has minimal effect on similarly treated PrP-/- samples (lanes 2 & 4). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-24786-1) licensed under a CC-BY license.Western Blot: Glut1 Antibody [NB110-39113] - Western blot of GLUT1 on mouse kidney membrane protein (lane A) and rat kidney membrane protein (lane B).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ChIP

     7 Reviews

62 Publications
NLRP3/NALP3 Antibody - BSA Free
NBP2-12446
C-AR inhibited NLRP3 inflammasome activation in synovial tissue. NLRP3 protein expression in succinate-stimulated synovial fibroblasts. The results were derived from four independent experiments for immunohistochemistry staining and Western blot and expressed as the mean +/- SD. *p < 0.05 vs. the model; #p < 0.05 vs. the indicated treatment. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fimmu.2016.00532/full), licensed under a CC-BY license.Analysis of human esophagus using NLRP3/NALP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in the squamous epithelium was observed. Staining was performed by Histowiz.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

     4 Reviews

232 Publications
IFN-alpha/beta R1 Antibody [Unconjugated]
AF245
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IFN‑ alpha / beta  R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=NoLineLink href=

Goat Polyclonal
Species Human
Applications WB, Flow, CyTOF-ready

9 Publications
Renin Antibody [Unconjugated] - 1
AF4277
Renin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Renin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=RLCs invade the glomerulus during EC model.A. Intraglomerular RLCs tagged by beta -gal, but negative for renin appear in the regenerative phase of the EC model (day 7). Representative confocal microscopy images for day 0 and day 7 of beta -gal/renin co-stained kidney slices. 4′,6-diamidino-2-phenylindole (DAPI) was used as a nuclear marker. The channels for green ( beta -gal) and red (renin) fluorescent signals in the dashed square on day 7 are separately shown in the small right panels. Scale bars correspond to 25 μm; B. Representative 3D reconstruction of glomeruli and beta -gal labelled RLCs (blue) on day 0 and day 7 of the EC model. The mesangial cell marker alpha 8-integrin (red) was used to visualize the glomeruli. Scale bars correspond to 20 μm; C. Quantification of glomeruli with tufts containing beta -gal expressing RLCs in the regenerative phase of the EC model (day 7). Data are presented as mean ± SEM, n = 5/10 for day 0 (baseline) /day 7, respectively. n.d.—not detectable; D. The intraglomerular RLCs observed during the EC model are not of hematopoietic origin. Representative confocal microscopy images for day 0 and day 7 of beta -gal/CD45 (hematopoietic marker) co-stained kidney slices. 4′,6-diamidino-2-phenylindole (DAPI) was used as a nuclear marker. The channels for green ( beta -gal) and red (CD45) fluorescent signals in the dashed square on day 7 are separately shown in the small right panels. Scale bars correspond to 25 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29771991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Goat Polyclonal
Species Mouse
Applications WB, IHC, IP

13 Publications
Neurophysin II/Arg-vasopressin Antibody [Unconjugated]
AF009
Neurophysin II was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human Neurophysin II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF009) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=NoLineLink href=

Goat Polyclonal
Species Human
Applications WB, IHC

HSD11B2 Antibody
NBP2-37898
Western Blot: HSD11B2 Antibody [NBP2-37898] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10<br/>Lane 2: Human Kidney tissueImmunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898] - Staining of human rectum.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

c-Fos Antibody (2H2)
NBP2-50037
Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Top panel: Analysis of c-Fos expression in HeLa cells using NBP2-50037. Lane 1: HeLa cells were serum-starved for 36 hours. Lane 2: Serum-starved HeLa cells were stimulated with 20% FBS (fetal bovine serum) for 2 hours. NBP2-50037 recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos. Serum starvation attenuates c-Fos expression, while 20% FBS strongly stimulates c-Fos expression. Bottom panel: Blot was stripped and probed with monoclonal antibody against GAPDH (NB300-221) used as loading control.Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN. DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

20 Publications
RAPGEF5 Antibody
NBP2-57362
Western Blot: RAPGEF5 Antibody [NBP2-57362] - Analysis in human cell line RT-4, human cell line U-251 MG and human plasma.Immunocytochemistry/Immunofluorescence: RAPGEF5 Antibody [NBP2-57362] - Staining of human cell line PC-3 shows localization to nucleoplasm & nuclear bodies.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF

Aquaporin 1/AQP1 Antibody (1/A5F6)
NB600-749
Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Pax8-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice, with no staining observed in glomeruli or bloods vessels (a, framed area is shown enlarged in b). Kidneys from vehicle treated mice were negative (c). Co-localisation studies revealed X-gal expression within tubules of all renal tubular compartment (proximal tubules-AQP1, distal tubules-THP and collecting ducts-AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY license.Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder. Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localized to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/doifinder/10.1038/srep11061), licensed under a CC-BY license.

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

     1 Review

14 Publications
V2 Vasopressin R/AVPR2 Antibody - BSA Free
NLS272
Immunohistochemistry-Paraffin: V2 Vasopressin R/AVPR2 Antibody [NLS272] - Colon, carcinomaImmunohistochemistry-Paraffin: V2 Vasopressin R/AVPR2 Antibody [NLS272] - Analysis of anti-AVPR2 antibody with human kidney, renal tubules.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications IHC, IHC-Fr, IHC-P

1 Publication