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![Western Blot: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY V-type proton ATPase subunit F (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-V-type proton ATPase subunit F.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-1B8-Western-Blot-NBP2-03498-img0002.jpg "Western Blot: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY V-type proton ATPase subunit F (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-V-type proton ATPase subunit F.")
![Immunocytochemistry/Immunofluorescence: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] Staining of COS7 cells transiently transfected by pCMV6-ENTRY V-type proton ATPase subunit F.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-1B8-Immunocytochemistry-Immunofluorescence-NBP2-03498-img0011.jpg)
![Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human thyroid tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03498-img0010.jpg)
![Flow Cytometry: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-V-type proton ATPase subunit F antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-1B8-Flow-Cytometry-NBP2-03498-img0001.jpg)
![Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03498-img0003.jpg)
![Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03498-img0004.jpg)
![Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human Kidney tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03498-img0005.jpg)
![Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human liver tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03498-img0006.jpg)
![Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human lung tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03498-img0007.jpg)
![Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human lymph node tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03498-img0008.jpg)
![Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human prostate tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03498-img0009.jpg)
![Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP1-88895] - Staining of human kidney, liver, lymph node and testis using Anti-ATP6V1D antibody NBP1-88895 (A) shows similar protein distribution across tissues to independent antibody NBP2-49254 (B).](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Immunohistochemistry-Paraffin-NBP1-88895-img0007.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP1-88895] - Staining of human kidney, liver, lymph node and testis using Anti-ATP6V1D antibody NBP1-88895 (A) shows similar protein distribution across tissues to independent antibody NBP2-49254 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: ATP6V1D Antibody [NBP1-88895] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Western-Blot-NBP1-88895-img0005.jpg)
![Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP1-88895] - Staining of human liver.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Immunohistochemistry-Paraffin-NBP1-88895-img0011.jpg)
![Western Blot: ATP6V1D Antibody [NBP1-88895] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Western-Blot-NBP1-88895-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP1-88895] - Staining of human testis.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Immunohistochemistry-Paraffin-NBP1-88895-img0008.jpg)
![Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP1-88895] - Staining of human lymph node.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Immunohistochemistry-Paraffin-NBP1-88895-img0009.jpg)
![Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP1-88895] - Staining of human kidney.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Immunohistochemistry-Paraffin-NBP1-88895-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: ATP6V1D Antibody [NBP2-49254] - Analysis of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49254-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATP6V1D Antibody [NBP2-49254] - Analysis of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.")
![Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254] - Staining of human liver.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Immunohistochemistry-Paraffin-NBP2-49254-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254] - Staining of human kidney shows strong luminal membranous positivity in proximal tubules.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Immunohistochemistry-Paraffin-NBP2-49254-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254] - Staining of human kidney, liver, lymph node and testis using Anti-ATP6V1D antibody NBP2-49254 (A) shows similar protein distribution across tissues to independent antibody NBP1-88895 (B).](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Immunohistochemistry-Paraffin-NBP2-49254-img0003.jpg)
![Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254] - Staining of human lymph node.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Immunohistochemistry-Paraffin-NBP2-49254-img0004.jpg)
![Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254] - Staining of human kidney.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Immunohistochemistry-Paraffin-NBP2-49254-img0005.jpg)
![Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254] - Staining of human testis.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Immunohistochemistry-Paraffin-NBP2-49254-img0007.jpg)
![Western Blot: ATP6V1D Antibody [NBP2-15518] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Western-Blot-NBP2-15518-img0004.jpg "Western Blot: ATP6V1D Antibody [NBP2-15518] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000")
![Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-15518] - V-ATPase D antibody detects ATP6V1D protein at cytoplasm on Breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded Breast carcinoma. V-ATPase D antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Immunohistochemistry-Paraffin-NBP2-15518-img0003.jpg)
![Western Blot: ATP6V1D Antibody [NBP2-15518] - V-ATPase D antibody detects ATP6V1D protein by Western blot analysis. A. 30 ug U87-MG whole cell lysate/extract 12 % SDS-PAGEV-ATPase D antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Western-Blot-NBP2-15518-img0002.jpg)
![Western Blot: V-type proton ATPase subunit F Antibody [NBP2-38942] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-Western-Blot-NBP2-38942-img0002.jpg "Western Blot: V-type proton ATPase subunit F Antibody [NBP2-38942] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-38942] - Staining in human cerebral cortex and skeletal muscle tissues using anti-ATP6V1F antibody. Corresponding ATP6V1F RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-Immunohistochemistry-Paraffin-NBP2-38942-img0006.jpg)
![Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-38942] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-Immunohistochemistry-Paraffin-NBP2-38942-img0003.jpg)
![Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-38942] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-Immunohistochemistry-Paraffin-NBP2-38942-img0004.jpg)
![Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-38942] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-Immunohistochemistry-Paraffin-NBP2-38942-img0005.jpg)
![Western Blot: ATP6V1D Antibody [NBP2-92709] - Analysis of extracts of various cell lines, using ATP6V1D at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Western-Blot-NBP2-92709-img0001.jpg "Western Blot: ATP6V1D Antibody [NBP2-92709] - Analysis of extracts of various cell lines, using ATP6V1D at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Immunohistochemistry-Paraffin: ATP6V1D Antibody (3G4) [H00051382-M01] - Analysis of monoclonal antibody to ATP6V1D on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.2 ug/ml.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-3G4-Immunohistochemistry-Paraffin-H00051382-M01-img0003.jpg "Immunohistochemistry-Paraffin: ATP6V1D Antibody (3G4) [H00051382-M01] - Analysis of monoclonal antibody to ATP6V1D on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.2 ug/ml.")
![Western Blot: ATP6V1A Antibody [NBP1-33021] - Immunoblot for Voa and V1A subunits indicate presence of subunits during the CCV isolation procedure. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/32569#content) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATP6V1A-Antibody-Western-Blot-NBP1-33021-img0014.jpg "Western Blot: ATP6V1A Antibody [NBP1-33021] - Immunoblot for Voa and V1A subunits indicate presence of subunits during the CCV isolation procedure. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/32569#content) licensed under a CC-BY license.")
![Immunohistochemistry: ATP6V1A Antibody [NBP1-33021] - Mouse prostate. ATP6V1A antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ATP6V1A-Antibody-Immunohistochemistry-NBP1-33021-img0008.jpg)
![Western Blot: ATP6V1A Antibody [NBP1-33021] - Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/ATP6V1A-Antibody-Western-Blot-NBP1-33021-img0006.jpg)
![Western Blot: ATP6V1A Antibody [NBP1-33021] - Various whole cell extracts (30ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody diluted by 1:10000.](http://images.novusbio.com/fullsize/ATP6V1A-Antibody-Western-Blot-NBP1-33021-img0011.jpg)
![Immunoprecipitation: ATP6V1A Antibody [NBP1-33021] - ATP6V1A Antibody [NBP1-33021] - Immunoprecipitation of ATP6V1A protein from HeLa whole cell extracts using 5 ug of ATP6V1A antibody. Western blot analysis was performed using ATP6V1A antibody EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/ATP6V1A-Antibody-Immunoprecipitation-NBP1-33021-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: ATP6V1A Antibody [NBP1-33021] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATP6V1A-Antibody-Western-Blot-NBP1-33021-img0013.jpg)
![Western Blot: ATP6V1A Antibody [NBP1-33021] - Functional analysis of the vATPase on CCVs.(A) Immunoblots of isolated SVs & CCVs for the clathrin light (LC) & heavy chains (HC), & SV marker proteins synaptophysin (Syph) & VAMP2 (left panel), as well as various Vo & V1 subunits of vATPase (right panel). (B) The ratio of the Vo & V1 as well as synaptophysin & VAMP2 (as SV markers) detected by immunoblotting in equal protein amount of CCVs & SVs (C) Normalized levels of Vo & V1 in CCV & SV samples, indicating that Vo:V1 ratio is 1:1 in both preparations. (D) ATPase activity measured in 1.3 µg of isolated SVs & CCVs. (E) Blocking percentage of ATPase activity by NEM (vATPase inhibitor) in 1.3 µg of SVs & CCVs. Error bars in (B–E) represent SD of 3–4 experimental replicates (p0.05 for C).Both Vo & V1 subunits are present on CCVs.Immunoblot for Voa & V1A subunits indicate presence of subunits during the CCV isolation procedure.ATPase activity measurements in isolated SVs.To make sure that the measured ATPase activity is within the standard curve range of the used kit, we tested different amounts of isolated SVs. The measurements of ATPase activity were then performed with 1.3 µg of SVs & CCVs which is in the liner range (inset). Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/32569), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33021_rabbit-polyclonal-atp6v1a-antibody-310202416182627.jpg)
![Immunocytochemistry/Immunofluorescence: V-type proton ATPase subunit F Antibody [NBP2-97190] - Immunofluorescence staining of V-type proton ATPase subunit F in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human V-type proton ATPase subunit F polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97190-img0001.jpg "Immunocytochemistry/Immunofluorescence: V-type proton ATPase subunit F Antibody [NBP2-97190] - Immunofluorescence staining of V-type proton ATPase subunit F in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human V-type proton ATPase subunit F polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-97190] - Immunochemical staining of human V-type proton ATPase subunit F in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-Immunohistochemistry-Paraffin-NBP2-97190-img0003.jpg)
![Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody [NBP2-97190] - Immunochemical staining of human V-type proton ATPase subunit F in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-Immunohistochemistry-Paraffin-NBP2-97190-img0002.jpg)
![Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-97189] - Immunochemical staining of human ATP6V1D in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATP6V1D-Antibody-Immunohistochemistry-Paraffin-NBP2-97189-img0001.jpg "Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-97189] - Immunochemical staining of human ATP6V1D in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Western Blot: V-type proton ATPase subunit F Antibody [NBP2-15160] - Sample (30 ug of whole cell lysate) A: U87-MG 15% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-Western-Blot-NBP2-15160-img0001.jpg "Western Blot: V-type proton ATPase subunit F Antibody [NBP2-15160] - Sample (30 ug of whole cell lysate) A: U87-MG 15% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry: V-type proton ATPase subunit F Antibody [NBP2-38587] - Staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/V-type-proton-ATPase-subunit-F-Antibody-Immunohistochemistry-NBP2-38587-img0002.jpg "Immunohistochemistry: V-type proton ATPase subunit F Antibody [NBP2-38587] - Staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.")