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![Western Blot: UBL5 Antibody [NBP2-86885] - WB Suggested Anti-Ubl5 Antibody. Titration: 1.0 ug/ml. Positive Control: Rat Liver](http://images.novusbio.com/fullsize/UBL5-Antibody-Western-Blot-NBP2-86885-img0003.jpg "Western Blot: UBL5 Antibody [NBP2-86885] - WB Suggested Anti-Ubl5 Antibody. Titration: 1.0 ug/ml. Positive Control: Rat Liver")
![Immunohistochemistry-Paraffin: UBL5 Antibody [NBP2-86885] - Rabbit Anti-Ubl5 antibody. Formalin Fixed Paraffin Embedded Tissue: Human Testis. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20x.](http://images.novusbio.com/fullsize/UBL5-Antibody-Immunohistochemistry-Paraffin-NBP2-86885-img0002.jpg)
![Immunohistochemistry-Paraffin: UBL5 Antibody [NBP2-86885] - Rabbit Anti-Ubl5 antibody. Formalin Fixed Paraffin Embedded Tissue: Human Kidney. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20x.](http://images.novusbio.com/fullsize/UBL5-Antibody-Immunohistochemistry-Paraffin-NBP2-86885-img0001.jpg)
![Western Blot: UBL5 Antibody (2F7) [H00059286-M01] - Detection against Immunogen (33.77 KDa) .](http://images.novusbio.com/fullsize/UBL5-Antibody-2F7-Western-Blot-H00059286-M01-img0001.jpg "Western Blot: UBL5 Antibody (2F7) [H00059286-M01] - Detection against Immunogen (33.77 KDa) .")
![Western Blot: UBL5 Antibody [NB600-470] - Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with Hub1 using a 1:500 dilution of IgG fraction of Rabbit-anti-Hub1 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4 C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-Hub1 is highly specific and does not cross react with other UBLs.](http://images.novusbio.com/fullsize/UBL5-Antibody-Western-Blot-NB600-470-img0003.jpg "Western Blot: UBL5 Antibody [NB600-470] - Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with Hub1 using a 1:500 dilution of IgG fraction of Rabbit-anti-Hub1 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4 C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-Hub1 is highly specific and does not cross react with other UBLs.")
![Western Blot: UBL5 Antibody [NB600-470] - Anti-Hub1 raised from recombinant yeast Hub1. All UBLs possess limited homology to Ubiquitin & to each other; hence it is important to know degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins after reaction with 1:1,000 dilution of anti-GFP followed by reaction with 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with Hub1 using 1:500 dilution of Rabbit-anti-Hub1 (Yeast) followed by reaction with 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primaries were diluted in TTBS buffer with 5% non-fat milk & incubated with membranes overnight at 4C. Yeast lysate proteins separated by SDS-PAGE using a 15% gel. Data indicates that anti-Hub1 is highly specific & does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche).](http://images.novusbio.com/fullsize/UBL5-Antibody-Western-Blot-NB600-470-img0001.jpg)
![Western Blot: UBL5 Overexpression Lysate (Adult Normal) [NBL1-17556] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBL5.](http://images.novusbio.com/fullsize/UBL5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17556-img0002.jpg "Western Blot: UBL5 Overexpression Lysate (Adult Normal) [NBL1-17556] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBL5.")
![Western Blot: UBL5 Overexpression Lysate (Adult Normal) [NBL1-17555] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBL5.](http://images.novusbio.com/fullsize/UBL5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17555-img0002.jpg "Western Blot: UBL5 Overexpression Lysate (Adult Normal) [NBL1-17555] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBL5.")
![SDS-Page: Recombinant Human UBL5 His Protein [NBP1-72328] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant Human UBL5 His Protein-SDS-Page-NBP1-72328-img0003.jpg "SDS-Page: Recombinant Human UBL5 His Protein [NBP1-72328] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")