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![Western Blot: SETD1A Antibody [NB100-558] - Detection of human hSET1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-hSET1 antibody A300-289A (lot A300-289A-6) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Western-Blot-NB100-558-img0016.jpg "Western Blot: SETD1A Antibody [NB100-558] - Detection of human hSET1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-hSET1 antibody A300-289A (lot A300-289A-6) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunohistochemistry-Paraffin: SETD1A Antibody [NB100-558] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-hSET1 used at 1:5,000 (0.2 ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunohistochemistry-Paraffin-NB100-558-img0017.jpg)
![Chromatin Immunoprecipitation: SETD1A Antibody [NB100-558] - Chromatin Immunoprecipitation: SETD1A Antibody [NB100-558] - ChIP-chip scatter plot of anti-hSET1 (NB100-558) enriched DNA binding sites versus input reference DNA. A. 10 mcg of NB100-558 was used to immunoprecipitate chromatin from K562 cells per Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-hSET1 ChIP, normal rabbit IgG showed little enrichment.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Chromatin-Immunoprecipitation-NB100-558-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: SETD1A Antibody [NB100-558] - Detection of human hSET1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-hSET1 antibody NB100-558 (lot NB100-558-6) used for IP at 6 ug per reaction. hSET1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-558-5) and another rabbit anti-hSET1 antibody. For blotting immunoprecipitated hSET1, the other antibody was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunoprecipitation-NB100-558-img0015.jpg)
![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining in human testis and pancreas tissues using anti-SETD1A antibody. Corresponding SETD1A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining in human testis and pancreas tissues using anti-SETD1A antibody. Corresponding SETD1A RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human cerebral cortex, kidney, pancreas and testis using Anti-SETD1A antibody NBP2-49281 (A) shows similar protein distribution across tissues to independent antibody NBP1-81513 (B).](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0006.jpg)
![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human Pancreas shows moderate nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0012.jpg)
![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0003.jpg)
![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0004.jpg)
![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0007.jpg)
![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human kidney.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0008.jpg)
![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human Cerebral cortex shows moderate nuclear positivity in neuronal and glial cells.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0009.jpg)
![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human cerebral cortex, kidney, pancreas and testis using Anti-SETD1A antibody NBP2-49281 (A) shows similar protein distribution across tissues to independent antibody NBP1-81513 (B).](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0010.jpg)
![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human Kidney shows moderate nuclear positivity in cells in tubules and glomeruli.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0011.jpg)
![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP2-49281] - Staining of human Testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP2-49281-img0013.jpg)
![Western Blot: SETD1A Antibody (BLR024E) [NBP2-76401] - Detection of human and mouse hSET1 by WB of HeLa, HEK293T, Jurkat, and TCMK-1 lysate. Antibody: Rabbit anti-hSET1 recombinant monoclonal [BLR024E] (NBP2-76401). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/SETD1A-Antibody-BLR024E-Western-Blot-NBP2-76401-img0001.jpg "Western Blot: SETD1A Antibody (BLR024E) [NBP2-76401] - Detection of human and mouse hSET1 by WB of HeLa, HEK293T, Jurkat, and TCMK-1 lysate. Antibody: Rabbit anti-hSET1 recombinant monoclonal [BLR024E] (NBP2-76401). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Immunohistochemistry-Paraffin: SETD1A Antibody (BLR024E) [NBP2-76401] - Section of human lung carcinoma. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody [BLR024E] used at 1:25. Secondary: HRPconjugated goat anti-rabbit IgG. (Substrate: DAB).](http://images.novusbio.com/fullsize/SETD1A-Antibody-BLR024E-Immunohistochemistry-NBP2-76401-img0003.jpg)
![Immunofluorescence: SETD1A Antibody (BLR024E) [NBP2-76401] - Detection of human hSET1 (red) in FFPE ovarian carcinoma by IHC-IF. Antibody: Rabbit anti-hSET1 recombinant monoclonal [BLR024E] (NBP2-76401). Secondary:DyLight 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/SETD1A-Antibody-BLR024E-Immunofluorescence-NBP2-76401-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: SETD1A Antibody (BLR024E) [NBP2-76401] - Detection of human hSET1 by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-hSET1 recombinant monoclonal [BLR024E] (NBP2-76401) and another rabbit anti-hSET1 antibody.](http://images.novusbio.com/fullsize/SETD1A-Antibody-BLR024E-Immunoprecipitation-NBP2-76401-img0004.jpg)
![Western Blot: SETD1A Antibody [NBP3-12907] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SETD1A antibody (NBP3-12907) diluted at 1:1000.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Western-Blot-NBP3-12907-img0001.jpg "Western Blot: SETD1A Antibody [NBP3-12907] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SETD1A antibody (NBP3-12907) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SETD1A Antibody [NBP3-12907] - SETD1A antibody detects SETD1A protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SETD1A protein stained by SETD1A antibody (NBP3-12907) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12907-img0002.jpg)
![Immunohistochemistry-Paraffin: SETD1A Antibody [NBP3-12907] - Mouse brain. SETD1A antibody (NBP3-12907) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunohistochemistry-Paraffin-NBP3-12907-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SETD1A Antibody [NBP3-12907] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETD1A antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-12907_rabbit-setd1a-pab-1610202419405790.jpg)
![Western Blot: SETD1A Antibody (7B7) [NBP2-45790] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD1A.](http://images.novusbio.com/fullsize/SETD1A-Antibody-7B7-Western-Blot-NBP2-45790-img0003.jpg "Western Blot: SETD1A Antibody (7B7) [NBP2-45790] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD1A.")
![Immunohistochemistry: SETD1A Antibody (7B7) [NBP2-45790] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SETD1A-Antibody-7B7-Immunohistochemistry-NBP2-45790-img0002.jpg)
![Immunohistochemistry: SETD1A Antibody (7B7) [NBP2-45790] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SETD1A-Antibody-7B7-Immunohistochemistry-NBP2-45790-img0001.jpg)
![Western Blot: SETD1A Antibody (PH00-02) [NBP3-32443] - Western blot analysis of SETD1A on 293T cell lysates with Rabbit anti-SETD1A antibody (NBP3-32443) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 186 kDa Observed band size: 300 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32443) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32443_rabbit-setd1a-mab-ph00-02-278202415242616.jpg "Western Blot: SETD1A Antibody (PH00-02) [NBP3-32443] - Western blot analysis of SETD1A on 293T cell lysates with Rabbit anti-SETD1A antibody (NBP3-32443) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 186 kDa Observed band size: 300 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32443) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: SETD1A Antibody (PH00-02) [NBP3-32443] - Flow cytometric analysis of 293T cells labeling SETD1A. Cells were fixed and permeabilized.Then stained with the primary antibody (NBP3-32443, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32443_rabbit-setd1a-mab-ph00-02-29202416372184.jpg)
![Western Blot: SETD1A Antibody [NBP3-18681] - Figure 2. Western blot analysis using the antibody directed against mouse SETD1A (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse SETD1A , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 186 kDa) is indicated on the right.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Western-Blot-NBP3-18681-img0001.jpg "Western Blot: SETD1A Antibody [NBP3-18681] - Figure 2. Western blot analysis using the antibody directed against mouse SETD1A (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse SETD1A , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 186 kDa) is indicated on the right.")
![ELISA: SETD1A Antibody [NBP3-18681] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse SETD1A . The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500.](http://images.novusbio.com/fullsize/SETD1A Antibody-ELISA-NBP3-18681-img0002.jpg)