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![Western Blot: CRALBP Antibody (B2) [NB100-74392] - Analysis of CRALBP antibody on human cell.](http://images.novusbio.com/fullsize/CRALBP-Antibody-B2-Western-Blot-NB100-74392-img0004.jpg "Western Blot: CRALBP Antibody (B2) [NB100-74392] - Analysis of CRALBP antibody on human cell.")
![Immunocytochemistry/Immunofluorescence: CRALBP Antibody (B2) [NB100-74392] - Immunostaining of CRALBP in frozen mouse retina sections.](http://images.novusbio.com/fullsize/CRALBP-Antibody-B2-Immunocytochemistry-Immunofluorescence-NB100-74392-img0003.jpg)
![Simple Western: CRALBP Antibody (B2) [NB100-74392] - iPSC-RPE from all donors express RPE marker proteins. Unlike iPSCs, all iPSC-RPE lines showed robust expression of CRALBP. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29540715/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CRALBP-Antibody-B2-Simple-Western-NB100-74392-img0005.jpg)
![Western Blot: CRALBP Antibody (1G6) [NBP2-00968] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRALBP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRALBP.](http://images.novusbio.com/fullsize/CRALBP-Antibody-1G6-Western-Blot-NBP2-00968-img0002.jpg "Western Blot: CRALBP Antibody (1G6) [NBP2-00968] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRALBP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRALBP.")
![Immunohistochemistry-Paraffin: CRALBP Antibody (1G6) [NBP2-00968] Staining of paraffin-embedded Human tonsil using anti-CRALBP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CRALBP-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00968-img0005.jpg)
![Flow Cytometry: CRALBP Antibody (1G6) [NBP2-00968] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRALBP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CRALBP-Antibody-1G6-Flow-Cytometry-NBP2-00968-img0001.jpg)
![Immunohistochemistry-Paraffin: CRALBP Antibody (1G6) [NBP2-00968] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CRALBP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CRALBP-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00968-img0003.jpg)
![Immunohistochemistry-Paraffin: CRALBP Antibody (1G6) [NBP2-00968] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CRALBP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CRALBP-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00968-img0004.jpg)
![Western Blot: CRALBP Antibody (2M0G2) [NBP3-16798] - Analysis of extracts of Mouse eye, using CRALBP Rabbit mAb (NBP3-16798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CRALBP-Antibody-2M0G2-Western-Blot-NBP3-16798-img0005.jpg "Western Blot: CRALBP Antibody (2M0G2) [NBP3-16798] - Analysis of extracts of Mouse eye, using CRALBP Rabbit mAb (NBP3-16798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: CRALBP Antibody (2M0G2) [NBP3-16798] - Immunofluorescence analysis of mouse eye using CRALBP Rabbit mAb (NBP3-16798) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CRALBP-Antibody-2M0G2-Immunohistochemistry-NBP3-16798-img0004.jpg)
![Immunohistochemistry-Paraffin: CRALBP Antibody (2M0G2) [NBP3-16798] - Rat retina using CRALBP Rabbit mAb (NBP3-16798) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CRALBP-Antibody-2M0G2-Immunohistochemistry-Paraffin-NBP3-16798-img0001.jpg)
![Immunohistochemistry-Paraffin: CRALBP Antibody (2M0G2) [NBP3-16798] - Mouse retina using CRALBP Rabbit mAb (NBP3-16798) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CRALBP-Antibody-2M0G2-Immunohistochemistry-Paraffin-NBP3-16798-img0002.jpg)
![Immunohistochemistry: CRALBP Antibody (2M0G2) [NBP3-16798] - Immunofluorescence analysis of rat eye using CRALBP Rabbit mAb (NBP3-16798) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CRALBP-Antibody-2M0G2-Immunohistochemistry-NBP3-16798-img0003.jpg)
![Immunohistochemistry-Paraffin: CRALBP Antibody [NBP2-58065] - Staining of human eye, retina, liver, lymphoid tissues and squamous epithelia using Anti-RLBP1 antibody NBP2-58065 (A) shows similar protein distribution across tissues to independent antibody (B).](http://images.novusbio.com/fullsize/CRALBP-Antibody-Immunohistochemistry-Paraffin-NBP2-58065-img0004.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CRALBP Antibody [NBP2-58065] - Staining of human eye, retina, liver, lymphoid tissues and squamous epithelia using Anti-RLBP1 antibody NBP2-58065 (A) shows similar protein distribution across tissues to independent antibody (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: CRALBP Antibody [NBP2-58065] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm, cytosol & centrosome.](http://images.novusbio.com/fullsize/CRALBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58065-img0001.jpg)
![Immunohistochemistry-Paraffin: CRALBP Antibody [NBP2-58065] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CRALBP-Antibody-Immunohistochemistry-Paraffin-NBP2-58065-img0007.jpg)
![Immunohistochemistry-Paraffin: CRALBP Antibody [NBP2-58065] - Staining of human retina shows strong positivity in pigment epithelium, Mueller glia and inner nuclear layer.](http://images.novusbio.com/fullsize/CRALBP-Antibody-Immunohistochemistry-Paraffin-NBP2-58065-img0002.jpg)
![Immunohistochemistry-Paraffin: CRALBP Antibody [NBP2-58065] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/CRALBP-Antibody-Immunohistochemistry-Paraffin-NBP2-58065-img0005.jpg)
![Immunohistochemistry-Paraffin: CRALBP Antibody [NBP2-58065] - Staining of human cervix shows no positivity in squmaous epithelial cells as expected.](http://images.novusbio.com/fullsize/CRALBP-Antibody-Immunohistochemistry-Paraffin-NBP2-58065-img0006.jpg)
![Western Blot: CRALBP Antibody [NBP1-31770] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CRALBP antibody [N2C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/CRALBP-Antibody-Western-Blot-NBP1-31770-img0004.jpg "Western Blot: CRALBP Antibody [NBP1-31770] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CRALBP antibody [N2C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/Immunofluorescence: CRALBP Antibody [NBP1-31770] - Analysis of methanol-fixed HepG2, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/CRALBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31770-img0002.jpg)
![Immunohistochemistry-Paraffin: CRALBP Antibody [NBP1-31770] - CRALBP antibody [N2C3] detects CRALBP protein by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: CRALBP stained by CRALBP antibody [N2C3] (NBP1-31770) diluted at 1:500.Red: beta Tubulin 3/ Tuj1 , a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31770_rabbit-polyclonal-cralbp-antibody-910202419593529.jpg)
![Immunohistochemistry-Paraffin: CRALBP Antibody [NBP1-31770] - CRALBP antibody [N2C3] detects CRALBP protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: CRALBP stained by CRALBP antibody [N2C3] (NBP1-31770) diluted at 1:500.Red: beta Tubulin 3/ Tuj1 , a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31770_rabbit-polyclonal-cralbp-antibody-910202419593597.jpg)
![Immunohistochemistry: CRALBP Antibody [NBP2-68644] - Immunohistochemical staining of human retina shows cytoplasmic positivity in all layers.](http://images.novusbio.com/fullsize/CRALBP-Antibody-Immunohistochemistry-NBP2-68644-img0001.jpg "Immunohistochemistry: CRALBP Antibody [NBP2-68644] - Immunohistochemical staining of human retina shows cytoplasmic positivity in all layers.")
![Western Blot: CRALBP Antibody (PSH0-74) [NBP3-32216] - Western blot analysis of CRALBP on different lysates with Rabbit anti-CRALBP antibody (NBP3-32216) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: HepG2 cell lysate (negative cell) Lane 3: Mouse eyeball tissue lysate Lane 4: Rat eyeball tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 36.5 kDa Observed band size: 30 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32216) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32216_rabbit-cralbp-mab-psh0-74-278202415242610.jpg "Western Blot: CRALBP Antibody (PSH0-74) [NBP3-32216] - Western blot analysis of CRALBP on different lysates with Rabbit anti-CRALBP antibody (NBP3-32216) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: HepG2 cell lysate (negative cell) Lane 3: Mouse eyeball tissue lysate Lane 4: Rat eyeball tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 36.5 kDa Observed band size: 30 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32216) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CRALBP Antibody (PSH0-74) [NBP3-32216] - Immunofluorescence analysis of paraffin-embedded mouse eyeball tissue labeling CRALBP with Rabbit anti-CRALBP antibody (NBP3-32216) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32216, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32216_rabbit-cralbp-mab-psh0-74-29820241426260.jpg)
![Immunohistochemistry: CRALBP Antibody (PSH0-74) [NBP3-32216] - Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue with Rabbit anti-CRALBP antibody (NBP3-32216) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32216) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32216_rabbit-cralbp-mab-psh0-74-29820241420923.jpg)
![Western Blot: CRALBP Antibody (JE35-09) [NBP3-32215] - Western blot analysis of CRALBP on different lysates with Rabbit anti-CRALBP antibody (NBP3-32215) at 1/500 dilution. Lane 1: Rat eyeball tissue lysate Lane 2: Mouse eyeball tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 36.5 kDa Observed band size: 36.5 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32215) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32215_rabbit-cralbp-mab-je35-09-278202416331297.jpg "Western Blot: CRALBP Antibody (JE35-09) [NBP3-32215] - Western blot analysis of CRALBP on different lysates with Rabbit anti-CRALBP antibody (NBP3-32215) at 1/500 dilution. Lane 1: Rat eyeball tissue lysate Lane 2: Mouse eyeball tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 36.5 kDa Observed band size: 36.5 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32215) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CRALBP Antibody (JE35-09) [NBP3-32215] - Immunocytochemistry analysis of B16F1 cells labeling CRALBP with Rabbit anti-CRALBP antibody (NBP3-32215) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CRALBP antibody (NBP3-32215) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32215_rabbit-cralbp-mab-je35-09-292024111488.jpg)
![Flow Cytometry: CRALBP Antibody (JE35-09) [NBP3-32215] - Flow cytometric analysis of B16F1 cells labeling CRALBP. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32215, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32215_rabbit-cralbp-mab-je35-09-29202416372145.jpg)
![Western Blot: CRALBP Antibody (4H5) [H00006017-M02] - RLBP1 monoclonal antibody (M02), clone 4H5 Analysis of RLBP1 expression in HepG2.](http://images.novusbio.com/fullsize/CRALBP-Antibody-4H5-Western-Blot-H00006017-M02-img0007.jpg "Western Blot: CRALBP Antibody (4H5) [H00006017-M02] - RLBP1 monoclonal antibody (M02), clone 4H5 Analysis of RLBP1 expression in HepG2.")
![Immunocytochemistry/Immunofluorescence: CRALBP Antibody (4H5) [H00006017-M02] - Analysis of monoclonal antibody to RLBP1 on HeLa cell. Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize/CRALBP-Antibody-4H5-Immunocytochemistry-Immunofluorescence-H00006017-M02-img0005.jpg)
![Sandwich ELISA: CRALBP Antibody (4H5) [H00006017-M02] - Detection limit for recombinant GST tagged RLBP1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CRALBP-Antibody-4H5-Sandwich-ELISA-H00006017-M02-img0006.jpg)
![Western Blot: CRALBP Antibody (SR1596) [NBP3-21908] - Western blot analysis using NBP3-21908 in mouse eyeball lysate.](http://images.novusbio.com/fullsize/nbp3-21908_rabbit-cralbp-mab-sr1596-57202315521817.jpg "Western Blot: CRALBP Antibody (SR1596) [NBP3-21908] - Western blot analysis using NBP3-21908 in mouse eyeball lysate.")
![SDS-Page: Recombinant Human CRALBP His Protein [NBP2-51538] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant Human CRALBP His Protein-SDS-Page-NBP2-51538-img0004.jpg "SDS-Page: Recombinant Human CRALBP His Protein [NBP2-51538] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![Western Blot: CRALBP Overexpression Lysate (Adult Normal) [NBL1-15383] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CRALBP.](http://images.novusbio.com/fullsize/CRALBP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15383-img0002.jpg "Western Blot: CRALBP Overexpression Lysate (Adult Normal) [NBL1-15383] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CRALBP.")