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![Western Blot: DNA polymerase alpha Antibody [NBP1-47257] - Human B cell lysate (40 ug). KSHV infection protects DNA polA1 from hypoxia mediated degradation. Mock or KSHV infected B cells were grown under normoxic or hypoxic condition followed by detecting DNA polA1 levels. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DNA-polymerase-alpha-Antibody-Western-Blot-NBP1-47257-img0002.jpg "Western Blot: DNA polymerase alpha Antibody [NBP1-47257] - Human B cell lysate (40 ug). KSHV infection protects DNA polA1 from hypoxia mediated degradation. Mock or KSHV infected B cells were grown under normoxic or hypoxic condition followed by detecting DNA polA1 levels. Western blot image submitted by a verified customer review.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: DNA polymerase alpha Antibody [NBP1-47257] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PolA1 antibody NBP1-47257 used for WB at 0.04 ug/mL (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody NBP1-47256, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/DNA-polymerase-alpha-Antibody-Western-Blot-NBP1-47257-img0001.jpg)
![Immunohistochemistry-Paraffin: DNA polymerase alpha Antibody [NBP1-82449] - Staining in human lymph node and liver tissues using anti-POLA1 antibody. Corresponding POLA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DNA-polymerase-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-82449-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DNA polymerase alpha Antibody [NBP1-82449] - Staining in human lymph node and liver tissues using anti-POLA1 antibody. Corresponding POLA1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DNA polymerase alpha Antibody [NBP1-82449] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/DNA-polymerase-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82449-img0006.jpg)
![Immunohistochemistry-Paraffin: DNA polymerase alpha Antibody [NBP1-82449] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/DNA-polymerase-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-82449-img0008.jpg)
![Immunohistochemistry-Paraffin: DNA polymerase alpha Antibody [NBP1-82449] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/DNA-polymerase-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-82449-img0009.jpg)
![Western Blot: DNA polymerase alpha Antibody [NBP1-47256] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PolA1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody NBP1-47257, which recognizes a downstream epitope](http://images.novusbio.com/fullsize/DNA-polymerase-alpha-Antibody-Western-Blot-NBP1-47256-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: DNA polymerase alpha Antibody [NBP1-47256] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PolA1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody NBP1-47257, which recognizes a downstream epitope")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: DNA polymerase alpha Antibody [NBP1-47256] - Analysis of DNA polymerase alpha in mouse A9 fibroblasts that were either Mock infected or were infected with the parvovirus MVM (Minute Virus of Mice). Image courtesy of Dr. Matt Fuller at University of Missouri-Columbia.](http://images.novusbio.com/fullsize/DNA-polymerase-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47256-img0002.jpg)
![SDS-Page: Recombinant Human DNA polymerase alpha Protein [H00005422-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005422-Q01-1.jpg "SDS-Page: Recombinant Human DNA polymerase alpha Protein [H00005422-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: DNA polymerase alpha Antibody (3C11) [H00005422-M01] - POLA monoclonal antibody (M01), clone 3C11 Analysis of POLA expression in Hela S3 NE.](http://images.novusbio.com/fullsize/DNA-polymerase-alpha-Antibody-3C11-Western-Blot-H00005422-M01-img0004.jpg "Western Blot: DNA polymerase alpha Antibody (3C11) [H00005422-M01] - POLA monoclonal antibody (M01), clone 3C11 Analysis of POLA expression in Hela S3 NE.")
![Sandwich ELISA: DNA polymerase alpha Antibody (3C11) [H00005422-M01] - Detection limit for recombinant GST tagged POLA is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DNA-polymerase-alpha-Antibody-3C11-Sandwich-ELISA-H00005422-M01-img0003.jpg)