Hu,
Mu,
Rt,
Bv,
Ce,
ChHa,
Pm,
RMSpecies Glossary
Reactivity: Hu, Mu, Rt, Bv, Ce, ChHa, Pm, RM
WB, Simple Western, Flow, Func, ICC/IF, IHC, Dual ISH-IHC, Single-Cell Western
Applications: WB, Simple Western, Flow, Func, ICC/IF, IHC, Dual ISH-IHC, Single-Cell Western
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody (NB110-89474) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD11b-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-89474-img0031.jpg "Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody (NB110-89474) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody conjugated to Alexa Fluor 488 (NB110-89474AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD11b-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-89474-img0028.jpg)
![Western Blot: CD11b Antibody - BSA Free [NB110-89474] - Western Blot detection of CD11b in RAW 264.7 whole cell lysates using [NB110-89474].](http://images.novusbio.com/fullsize/CD11b-Antibody---BSA-Free-Western-Blot-NB110-89474-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Staining of mouse pancreas using [NB110-89474] at 1:400 dilution. Nuclei counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD11b-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-89474-img0010.jpg)
![Immunohistochemistry: CD11b Antibody - BSA Free [NB110-89474] - Glibenclamide and Abcc8-/- suppress immune cell infiltration in EAE. White matter of lumbar spinal cord sections from WT control (a), untreated pid-30 WT/EAE (b), glibenclamide-treated pid-30 WT/EAE (c), and pid-30 Abcc8-/-/EAE (d) mice, stained with H&E or immunolabeled for CD45 (leucocyte), CD3 (T cells), CD20 (B cells), or CD11b (macrophage/microglia), as indicated; original magnification, x200 (H&E) or x400 (all immunolabelings). eleft panel: percent of quadrants with inflammatory cells on H&E; four mice/group. efour right panels: Quantification of CD-45-, CD3-, CD20-, and CD11b-expressing cells in white matter; four mice/group; ##P < 0.01 with respect to WT control; **P < 0.01, and ***P < 0.001 with respect to WT/EAE; scale bars, 100 um Image collected and cropped by CiteAb from the following publication (//www.jneuroinflammation.com/content/12/1/210) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD11b-Antibody---BSA-Free-Immunohistochemistry-NB110-89474-img0024.jpg)
![Flow (Cell Surface): CD11b Antibody - BSA Free [NB110-89474] - Surface staining of CD11b in CT26 colorectal carcinoma tumor model. Using Alexa Fluor 405 conjugated version of the antibody (NB110-89474AF405). Image from verified customer review.](http://images.novusbio.com/fullsize/CD11b-Antibody-BSA-Free-Flow-Cell-Surface-NB110-89474-img0033.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody conjugated to Alexa Fluor 647 (NB110-89474AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD11b-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-89474-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody conjugated to DyLight 650 (NB110-8947C) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD11b-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-89474-img0030.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - CD11b Antibody [NB110-89474] - CD11b antibody [NB110-89474] was tested in Raw264.7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/CD11b-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-89474-img0009.jpg)
![Immunohistochemistry: CD11b Antibody - BSA Free [NB110-89474] - Population of CD11b + myeloid progenitor cells differentiate into SMA + stromal cells within tumors and in vitro. Representative image of CD11b + SMA + double positive cells within tumor stroma. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32731354/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD11b Antibody - BSA Free-Immunohistochemistry-NB110-89474-img0034.jpg)
![Immunohistochemistry: CD11b Antibody - BSA Free [NB110-89474] - Immunohistochemical analysis of CD11b in human renal cancer with [NB110-89474] using 3,3'-Diaminobenzidine (DAB) with hematoxylin counterstain.](http://images.novusbio.com/fullsize/CD11b-Antibody---BSA-Free-Immunohistochemistry-NB110-89474-img0011.jpg)
![Immunohistochemistry-Frozen: CD11b Antibody - BSA Free [NB110-89474] - Immunohistochemical analysis of frozen sections of mouse spleen using [NB110-89474]. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD11b-Antibody---BSA-Free-Immunohistochemistry-Frozen-NB110-89474-img0021.jpg)
![Immunohistochemistry: CD11b Antibody - BSA Free [NB110-89474] - Representative photomicrographs of cross sections of uteri collected from wild type (WT) mice 24 h after intrauterine infusion with 100 ul of vehicle or 100 ug ultrapure LPS from E. coli O111:B4, and Tlr4-/- mice similarly infused intrauterine with vehicle or LPS. Cross sections were stained with haematoxylin and eosin, and immunostained using a CD11b antibody for granulocytes (red) and DAPI for cell nuclei (white), and images collected at 10X magnification sections (C, F, I, L; bars = 200 um). At least 4 sections per animal and at least 4 fields per section were examined. Only WT mice infused with LPS show evidence of inflammation and accumulation of granulocytes in the endometrium. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0012906) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD11b-Antibody---BSA-Free-Immunohistochemistry-NB110-89474-img0023.jpg)
![Flow (Cell Surface): CD11b Antibody - BSA Free [NB110-89474] - CD11b Antibody [NB110-89474] - A surface stain was performed on Raw264.7 cells with DyLight 550-conjugated [NB110-89474R] (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/fullsize/CD11b-Antibody-BSA-Free-Flow-Cell-Surface-NB110-89474-img0022.jpg)
![Flow (Cell Surface): CD11b Antibody - BSA Free [NB110-89474] - An intracellular stain was performed on THP-1 cells with CD11b NB110-89474 (blue) and a matched isotype control NBP2-24891 (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CD11b-Antibody-BSA-Free-Flow-Cell-Surface-NB110-89474-img0032.jpg)
![Simple Western: CD11b Antibody - BSA Free [NB110-89474] - Specific band for Cd11b in 1.0 mg/mL of Dentate Gyrus from Rat Brain. [NB110-89474] was used at a dilution of 1:50. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD11b-Antibody---BSA-Free-Simple-Western-NB110-89474-img0012.jpg)
![Dual RNAscope ISH-IHC: CD11b Antibody - BSA Free [NB110-89474] - Formalin-fixed paraffin-embedded tissue sections of human lymph node were probed for CD11b mRNA (ACD RNAScope Probe, catalog #555098; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog #NB110-89474) at 1:50 dilution with 1 hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.](http://images.novusbio.com/fullsize/CD11b-Antibody---BSA-Free-Dual-RNAscope-ISH-IHC-NB110-89474-img0025.jpg)
![Dual RNAscope ISH-IHC: CD11b Antibody - BSA Free [NB110-89474] - CD14 mRNA (red) and CD11b protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human malignant lymph node. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CD14 and CD11b antibody at 1:200 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNA scope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).](http://images.novusbio.com/fullsize/CD11b-Antibody---BSA-Free-Dual-RNAscope-ISH-IHC-NB110-89474-img0026.jpg)
![Western Blot: CD11b Antibody - BSA Free [NB110-89474] - Immunodensities of (a) CD11b, (b) GFAP & (c) NF-kappa B (p65) proteins with representative immunoblots in striatum from Munc18-OE (n = 5) & wild-type (n = 5) mice. Bar graphs are ratios of optical densities of our proteins of interest to beta -actin (42 kDa band), expressed as immunoreactivity in percentage of mean value of the WT group (100%). No differences were detected between groups for any of the analyzed proteins. Right panels are representative immunoblots for target proteins & beta -actin which included Munc18-OE (OE) & wild-type (WT) mice samples. The molecular masses were estimated from referenced standards. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25069615), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89474_rabbit-polyclonal-cd11b-antibody-31020241535670.jpg)
![Immunohistochemistry: CD11b Antibody - BSA Free [NB110-89474] - Comparison of Ki-67 or CD11b immunoreactivity of Aldara-treated dorsal skin samples of C57BL/6 mice using OP or MP. Nuclear proliferation marker Ki-67 immunostaining on OP (a) & MP (b) dorsal skin tissue samples at 400x magnification. Dermal dendritic cells were labelled with anti-CD11b antibody on OP (c) & MP samples (d) at 400x magnification. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30842501), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89474_rabbit-polyclonal-cd11b-antibody-310202415384158.jpg)
![Immunohistochemistry: CD11b Antibody - BSA Free [NB110-89474] - Comparison of Ki-67 or CD11b immunoreactivity of Aldara-treated dorsal skin samples of C57BL/6 mice using OP or MP. Nuclear proliferation marker Ki-67 immunostaining on OP (a) & MP (b) dorsal skin tissue samples at 400x magnification. Dermal dendritic cells were labelled with anti-CD11b antibody on OP (c) & MP samples (d) at 400x magnification. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30842501), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89474_rabbit-polyclonal-cd11b-antibody-31020241539450.jpg)
![Western Blot: CD11b Antibody - BSA Free [NB110-89474] - Immunodensities of (a) CD11b, (b) GFAP & (c) NF-kappa B (p65) proteins with representative immunoblots in cerebral cortex from Munc18-OE (n = 5) & wild-type (n = 5) mice. Bar graphs are ratios of optical densities of our proteins of interest to beta -actin (42 kDa band), expressed as immunoreactivity in percentage of the mean value of the WT group (100%). CD11b was significantly decreased in Munc18-OE mice compared to the WT (t = 3.01; P <0.05). No differences between groups were observed in the levels of GFAP or NF-kappa B. Right panels are representative immunoblots for target proteins & beta -actin which included Munc18-OE (OE) & wild-type (WT) mice samples. The molecular masses were estimated from referenced standards. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25069615), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89474_rabbit-polyclonal-cd11b-antibody-310202415394511.jpg)
![Immunocytochemistry/ Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - CD11b staining in IMhu. Cells were stained with antibodies against the CD11b surface antigen. (A) Panel A shows positive staining for CD11b & (B) panel B shows nuclear DAPI staining. Magnitude 40×. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31096716), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89474_rabbit-polyclonal-cd11b-antibody-310202415291998.jpg)
![Western Blot: CAB39 Antibody [NBP2-15658] - Sample (30 ug of whole cell lysate) A: HeLa B: HCT116 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CAB39-Antibody-Western-Blot-NBP2-15658-img0001.jpg "Western Blot: CAB39 Antibody [NBP2-15658] - Sample (30 ug of whole cell lysate) A: HeLa B: HCT116 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CAB39 Antibody [NBP2-15658] - CAB39 antibody detects CAB39 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CAB39 protein stained by CAB39 antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/CAB39-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15658-img0003.jpg)
![Immunohistochemistry-Paraffin: CAB39 Antibody [NBP2-15658] - Paraffin-embedded mouse testis. CAB39 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/CAB39-Antibody-Immunohistochemistry-Paraffin-NBP2-15658-img0005.jpg)
![Immunohistochemistry-Paraffin: CAB39 Antibody [NBP2-15658] - Analysis of paraffin-embedded TOV21G xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CAB39-Antibody-Immunohistochemistry-Paraffin-NBP2-15658-img0004.jpg)
![Western Blot: CAB39 Antibody (S03-4G7) [NBP3-19841] - Western blot detection of CAB395 in Hela, A549, HL-60, U2OS, U87-MG cell lysates using NBP3-19841 (1:1000 diluted). Predicted band size: 40kDa. Observed band size: 39kDa.](http://images.novusbio.com/fullsize/CAB39-Antibody-S03-4G7-Western-Blot-NBP3-19841-img0002.jpg "Western Blot: CAB39 Antibody (S03-4G7) [NBP3-19841] - Western blot detection of CAB395 in Hela, A549, HL-60, U2OS, U87-MG cell lysates using NBP3-19841 (1:1000 diluted). Predicted band size: 40kDa. Observed band size: 39kDa.")
![Immunohistochemistry-Paraffin: CAB39 Antibody (S03-4G7) [NBP3-19841] - Immunohistochemistry of CAB39 in paraffin-embedded Human Cholangiocarcinoma using NBP3-19841 at dilution 1/50](http://images.novusbio.com/fullsize/CAB39-Antibody-S03-4G7-Immunohistochemistry-Paraffin-NBP3-19841-img0001.jpg)
![Western Blot: CAB39 Antibody [NBP2-92297] - Analysis of extracts of U-87MG cells, using CAB39 .Exposure time: 5min.](http://images.novusbio.com/fullsize/CAB39-Antibody-Western-Blot-NBP2-92297-img0001.jpg "Western Blot: CAB39 Antibody [NBP2-92297] - Analysis of extracts of U-87MG cells, using CAB39 .Exposure time: 5min.")
![Western Blot: CAB39 Antibody [H00051719-B01P] - Analysis of CAB39 expression in transfected 293T cell line by CAB39 polyclonal antibody. Lane 1: CAB39 transfected lysate(37.51 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CAB39-Antibody-Western-Blot-H00051719-B01P-img0003.jpg "Western Blot: CAB39 Antibody [H00051719-B01P] - Analysis of CAB39 expression in transfected 293T cell line by CAB39 polyclonal antibody. Lane 1: CAB39 transfected lysate(37.51 KDa). Lane 2: Non-transfected lysate.")
![Proximity Ligation Assay: CAB39 Antibody [H00051719-B01P] - Analysis of protein-protein interactions between STK11 and CAB39. HeLa cells were stained with anti-STK11 rabbit purified polyclonal 1:1200 and anti-CAB39 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein in](http://images.novusbio.com/fullsize/CAB39-Antibody-Proximity-Ligation-Assay-H00051719-B01P-img0002.jpg)
![Western Blot: CAB39 Antibody [NBP1-55393] - Human Liver cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CAB39-Antibody-Western-Blot-NBP1-55393-img0002.jpg "Western Blot: CAB39 Antibody [NBP1-55393] - Human Liver cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: CAB39 Antibody [NBP3-12457] - 1:500 antibody dilution in DiluObuffer, Apparent MW is 39 KDa.](http://images.novusbio.com/fullsize/CAB39-Antibody-Western-Blot-NBP3-12457-img0001.jpg "Western Blot: CAB39 Antibody [NBP3-12457] - 1:500 antibody dilution in DiluObuffer, Apparent MW is 39 KDa.")
![Western Blot: CAB39 Overexpression Lysate (Adult Normal) [NBL1-08620] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CAB39.](http://images.novusbio.com/fullsize/CAB39-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08620-img0002.jpg "Western Blot: CAB39 Overexpression Lysate (Adult Normal) [NBL1-08620] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CAB39.")
![Western Blot: CAB39 Overexpression Lysate (Adult Normal) [NBP2-09524] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CAB39.](http://images.novusbio.com/fullsize/CAB39-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09524-img0001.jpg "Western Blot: CAB39 Overexpression Lysate (Adult Normal) [NBP2-09524] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CAB39.")
![Western Blot: CAB39 Overexpression Lysate (Adult Normal) [NBP2-09523] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CAB39.](http://images.novusbio.com/fullsize/CAB39-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09523-img0001.jpg "Western Blot: CAB39 Overexpression Lysate (Adult Normal) [NBP2-09523] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CAB39.")
![Western Blot: CAB39 Antibody [H00051719-D01P] - Analysis of CAB39 expression in transfected 293T cell line by CAB39 polyclonal antibody.Lane 1: CAB39 transfected lysate(39.90 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CAB39-Antibody-Western-Blot-H00051719-D01P-img0001.jpg "Western Blot: CAB39 Antibody [H00051719-D01P] - Analysis of CAB39 expression in transfected 293T cell line by CAB39 polyclonal antibody.Lane 1: CAB39 transfected lysate(39.90 KDa).Lane 2: Non-transfected lysate.")
![SDS-Page: Recombinant Human CAB39 Protein [NBP1-50834] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-CAB39-Protein-SDS-Page-NBP1-50834-img0001.jpg "SDS-Page: Recombinant Human CAB39 Protein [NBP1-50834] - 15 % SDS-PAGE (3ug)")