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![Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP2-32691] - Analysis in human liver and pancreas tissues using NBP2-32691 antibody. Corresponding GSTO1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Immunohistochemistry-Paraffin-NBP2-32691-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP2-32691] - Analysis in human liver and pancreas tissues using NBP2-32691 antibody. Corresponding GSTO1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP2-32691] - Staining of human kidney, liver, pancreas and testis using Anti-GSTO1 antibody NBP2-32691 (A) shows similar protein distribution across tissues to independent antibody NBP1-83321 (B).](http://images.novusbio.com/fullsize/GSTO1-Antibody-Immunohistochemistry-Paraffin-NBP2-32691-img0017.jpg)
![Western Blot: GSTO1 Antibody [NBP2-32691] - Human breast cancer cell line MCF7 was treated with carboplatin for 72 hours and expression of GSTO1 was detected by Western blot. Image from verified customer review.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Western-Blot-NBP2-32691-img0012.jpg)
![Western Blot: GSTO1 Antibody [NBP2-32691] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/GSTO1-Antibody-Western-Blot-NBP2-32691-img0004.jpg)
![Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP2-32691] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Immunohistochemistry-Paraffin-NBP2-32691-img0009.jpg)
![Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP2-32691] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Immunohistochemistry-Paraffin-NBP2-32691-img0010.jpg)
![Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP2-32691] - Staining of human kidney.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Immunohistochemistry-Paraffin-NBP2-32691-img0014.jpg)
![Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP2-32691] - Staining of human testis.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Immunohistochemistry-Paraffin-NBP2-32691-img0015.jpg)
![Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP2-32691] - Staining of human thyroid gland.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Immunohistochemistry-Paraffin-NBP2-32691-img0016.jpg)
![Western Blot: GSTO1 Antibody [NBP1-83321] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/GSTO1-Antibody-Western-Blot-NBP1-83321-img0004.jpg "Western Blot: GSTO1 Antibody [NBP1-83321] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP1-83321] - Analysis in human liver and pancreas tissues using NBP1-83321 antibody. Corresponding GSTO1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Immunohistochemistry-Paraffin-NBP1-83321-img0013.jpg)
![Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP1-83321] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Immunohistochemistry-Paraffin-NBP1-83321-img0005.jpg)
![Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP1-83321] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Immunohistochemistry-Paraffin-NBP1-83321-img0006.jpg)
![Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP1-83321] - Staining of human testis.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Immunohistochemistry-Paraffin-NBP1-83321-img0009.jpg)
![Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP1-83321] - Staining of human thyroid gland.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Immunohistochemistry-Paraffin-NBP1-83321-img0010.jpg)
![Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP1-83321] - Staining of human kidney.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Immunohistochemistry-Paraffin-NBP1-83321-img0011.jpg)
![Knockout Validated: GSTO1 Antibody [NBP1-33763] - Wild-type (WT) and GSTO1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GSTO1 antibody [N1C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Western-Blot-NBP1-33763-img0008.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: GSTO1 Antibody [NBP1-33763] - Wild-type (WT) and GSTO1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GSTO1 antibody [N1C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Western Blot: GSTO1 Antibody [NBP1-33763] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GSTO1 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Western-Blot-NBP1-33763-img0005.jpg)
![Immunohistochemistry-Paraffin: GSTO1 Antibody [NBP1-33763] - Paraffin-embedded GSTA2 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Immunohistochemistry-Paraffin-NBP1-33763-img0002.jpg)
![Western Blot: GSTO1 Antibody [NBP1-33763] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/GSTO1-Antibody-Western-Blot-NBP1-33763-img0003.jpg)
![Western Blot: GSTO1 Antibody (2C7) [NBP3-15189] - Positive WB detected in: Jurkat whole cell lysate, PC3 whole cell lysate, Raji whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate. All lanes: GSTO1 antibody at 1.25ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 28, 24, 25 KDa. Observed band size: 28 KDa.](http://images.novusbio.com/fullsize/GSTO1-Antibody-2C7-Western-Blot-NBP3-15189-img0004.jpg "Western Blot: GSTO1 Antibody (2C7) [NBP3-15189] - Positive WB detected in: Jurkat whole cell lysate, PC3 whole cell lysate, Raji whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate. All lanes: GSTO1 antibody at 1.25ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 28, 24, 25 KDa. Observed band size: 28 KDa.")
![Immunocytochemistry/Immunofluorescence: GSTO1 Antibody (2C7) [NBP3-15189] - Immunofluorescence staining of HepG2 cells with at GSTO1 antibody 1:41, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/GSTO1-Antibody-2C7-Immunocytochemistry-Immunofluorescence-NBP3-15189-img0001.jpg)
![Immunohistochemistry: GSTO1 Antibody (2C7) [NBP3-15189] - IHC image of GSTO1 antibody diluted at 1:125 and staining in paraffin-embedded human liver cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/GSTO1-Antibody-2C7-Immunohistochemistry-NBP3-15189-img0003.jpg)
![Immunohistochemistry: GSTO1 Antibody (2C7) [NBP3-15189] - IHC image of GSTO1 antibody diluted at 1:125 and staining in paraffin-embedded human liver tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/GSTO1-Antibody-2C7-Immunohistochemistry-NBP3-15189-img0002.jpg)
![SDS-Page: GSTO1 Antibody (14A9.F6) [NBP2-44176] - Lane 1: Non-reduced Mouse anti-GSTO1 Monoclonal Antibody. Lane 2: 3 UL OPAL Pre-stained Marker. Lane 3: Reduced Mouse anti-GSTO1 Monoclonal Antibody. Load: 1 Ug per lane. Predicted/Observed size: Non-reduced at 160 kDa/observed at 180-200 kDa; Reduced at 55, 25 kDa. Non-reduced migrates at slightly higher molecular weight.](http://images.novusbio.com/fullsize/GSTO1-Antibody-14A9-F6-SDS-Page-NBP2-44176-img0001.jpg "SDS-Page: GSTO1 Antibody (14A9.F6) [NBP2-44176] - Lane 1: Non-reduced Mouse anti-GSTO1 Monoclonal Antibody. Lane 2: 3 UL OPAL Pre-stained Marker. Lane 3: Reduced Mouse anti-GSTO1 Monoclonal Antibody. Load: 1 Ug per lane. Predicted/Observed size: Non-reduced at 160 kDa/observed at 180-200 kDa; Reduced at 55, 25 kDa. Non-reduced migrates at slightly higher molecular weight.")
![Western Blot: GSTO1 Antibody (SR1468) [NBP3-21958] - Western blot analysis using NBP3-21958 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21958_rabbit-gsto1-mab-sr1468-57202316494229.jpg "Western Blot: GSTO1 Antibody (SR1468) [NBP3-21958] - Western blot analysis using NBP3-21958 in HepG2 cell lysate.")
![Sandwich ELISA: GSTO1 Antibody (3D9) [H00009446-M01] - Detection limit for recombinant GST tagged GSTO1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GSTO1-Antibody-3D9-Sandwich-ELISA-H00009446-M01-img0004.jpg "Sandwich ELISA: GSTO1 Antibody (3D9) [H00009446-M01] - Detection limit for recombinant GST tagged GSTO1 is approximately 0.03ng/ml as a capture antibody.")
![Immunoprecipitation: GSTO1 Antibody (3D9) [H00009446-M01] - Analysis of GSTO1 transfected lysate using anti-GSTO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GSTO1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/GSTO1-Antibody-3D9-Immunoprecipitation-H00009446-M01-img0003.jpg)
![Western Blot: GSTO1 Antibody (SR1467) [NBP3-22466] - Western blot analysis using NBP3-22466 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22466_rabbit-gsto1-mab-sr1467-6720239533798.jpg "Western Blot: GSTO1 Antibody (SR1467) [NBP3-22466] - Western blot analysis using NBP3-22466 in HeLa cell lysate.")
![Western Blot: GSTO1 Antibody (7L9P3) [NBP3-16453] - Western blot analysis of extracts of various cell lines, using GSTO1 Rabbit mAb (NBP3-16453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/GSTO1-Antibody-7L9P3-Western-Blot-NBP3-16453-img0001.jpg "Western Blot: GSTO1 Antibody (7L9P3) [NBP3-16453] - Western blot analysis of extracts of various cell lines, using GSTO1 Rabbit mAb (NBP3-16453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: GSTO1 Overexpression Lysate (Adult Normal) [NBP2-07819] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GSTO1.](http://images.novusbio.com/fullsize/GSTO1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07819-img0001.jpg "Western Blot: GSTO1 Overexpression Lysate (Adult Normal) [NBP2-07819] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GSTO1.")
![ELISA: GSTO1 Antibody (1B6) [H00009446-M03] - Detection limit for recombinant GST tagged GSTO1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GSTO1-Antibody-1B6-ELISA-H00009446-M03-img0001.jpg "ELISA: GSTO1 Antibody (1B6) [H00009446-M03] - Detection limit for recombinant GST tagged GSTO1 is approximately 0.03ng/ml as a capture antibody.")