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![Knockout Validated: Carm1 Antibody [NB100-56374] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Carm1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human Carm1 Polyclonal Antibody (Catalog # NB100-56374) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Carm1 at approximately 65 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Carm1-Antibody-Knockout-Validated-NB100-56374-img0006.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Carm1 Antibody [NB100-56374] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Carm1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human Carm1 Polyclonal Antibody (Catalog # NB100-56374) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Carm1 at approximately 65 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Carm1 Antibody [NB100-56374] - Analysis of PRMT4 in cell lysates from HeLa cells using this antibody. 5 ug/ml. Twenty ug of HeLa cell lysate was loaded per well of mini gel.](http://images.novusbio.com/fullsize/Carm1-Antibody-Western-Blot-NB100-56374-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Carm1 Antibody [NB100-56374] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Carm1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56374-img0005.jpg)
![Immunohistochemistry-Paraffin: Carm1 Antibody [NB100-56374] - Analysis of a FFPE tissue section of the human breast carcinoma using 1:200 dilution of Carm1 antibody (NB100-56374). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated nuclear and cytoplasmic staining.](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunohistochemistry-Paraffin-NB100-56374-img0004.jpg)
![Knockdown Validated: Carm1 Antibody [NB200-342] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Carm1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:5000 ug/ml of Rabbit Anti-Human Carm1 Polyclonal Antibody (Catalog # NB200-342) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Carm1 at approximately 65 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Carm1-Antibody-Knockdown-Validated-NB200-342-img0013.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: Carm1 Antibody [NB200-342] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Carm1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:5000 ug/ml of Rabbit Anti-Human Carm1 Polyclonal Antibody (Catalog # NB200-342) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Carm1 at approximately 65 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Carm1 Antibody [NB200-342] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Carm1-Antibody-Western-Blot-NB200-342-img0015.jpg)
![Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-342] - Detection of human CARM1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CARM1 (Cat. No. A300-421A lot 4) used at 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunohistochemistry-Paraffin-NB200-342-img0016.jpg)
![Western Blot: Carm1 Antibody [NB200-342] - Whole cell lysate (10 ug) from HEK293T, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Carm1-Antibody-Western-Blot-NB200-342-img0014.jpg)
![Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-342] - Section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunohistochemistry-Paraffin-NB200-342-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Carm1 Antibody [NB200-342] - Detection of human CARM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARM1 antibody NB200-342 (lot NB200-342-4) used for IP at 6 ug per reaction. CARM1 was also immunoprecipitated by a previous lot of this antibody (lot NB200-342-3) and another rabbit anti-CARM1 antibody. For blotting immunoprecipitated CARM1, NB200-342 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunoprecipitation-NB200-342-img0012.jpg)
![Western Blot: Carm1 Antibody (3H2) [NBP2-37645] - Western blot analysis using CARM1 mouse mAb against MCF-7 (1), Hela (2), NIH/3T3 (3), HL-60 (4), LNcap (5), Jurkat (6), PC-3 (7), Cos7 (8), and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize/Carm1-Antibody-3H2-Western-Blot-NBP2-37645-img0001.jpg "Western Blot: Carm1 Antibody (3H2) [NBP2-37645] - Western blot analysis using CARM1 mouse mAb against MCF-7 (1), Hela (2), NIH/3T3 (3), HL-60 (4), LNcap (5), Jurkat (6), PC-3 (7), Cos7 (8), and PC-12 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Carm1 Antibody (3H2) [NBP2-37645] - Immunofluorescence analysis of Hela cells using CRAM1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Carm1-Antibody-3H2-Immunocytochemistry-Immunofluorescence-NBP2-37645-img0002.jpg)
![Immunohistochemistry: Carm1 Antibody (3H2) [NBP2-37645] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) using CARM1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Carm1-Antibody-3H2-Immunohistochemistry-NBP2-37645-img0004.jpg)
![Flow Cytometry: Carm1 Antibody (3H2) [NBP2-37645] - Flow cytometric analysis of Lovo cells using CARM1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Carm1-Antibody-3H2-Flow-Cytometry-NBP2-37645-img0003.jpg)
![Western Blot: Carm1 Antibody [NB200-341] - Detection of Human and Mouse CARM1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody NB200-341 used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Carm1-Antibody-Western-Blot-NB200-341-img0004.jpg "Western Blot: Carm1 Antibody [NB200-341] - Detection of Human and Mouse CARM1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody NB200-341 used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-341] - Human stomach carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunohistochemistry-Paraffin-NB200-341-img0002.jpg)
![Western Blot: Carm1 Antibody [NB200-341] - Whole HeLa cell lysate (25 mcg for WB in A; 5 mg for IP in B).antibody used at 0.1 mcg/ml for WB and at 4 mcg/5 mg lysate for IP. Detection: Chemiluminescence A. Coomassie stain in B. Band was verified by mass spectrometry.](http://images.novusbio.com/fullsize/Carm1-Antibody-Western-Blot-NB200-341-img0001.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21326_rabbit-carm1-pab-45202315514713.jpg)
![Immunocytochemistry/Immunofluorescence: Carm1 Antibody [NBP2-98754] - Immunofluorescence staining of Carm1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human Carm1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98754-img0001.jpg "Immunocytochemistry/Immunofluorescence: Carm1 Antibody [NBP2-98754] - Immunofluorescence staining of Carm1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human Carm1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.")
![Immunohistochemistry-Paraffin: Carm1 Antibody [NBP2-98754] - Immunochemical staining of human Carm1 in human ovarian cancer with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunohistochemistry-Paraffin-NBP2-98754-img0003.jpg)
![Immunohistochemistry-Paraffin: Carm1 Antibody [NBP2-98754] - Immunochemical staining of human Carm1 in human prostatic carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunohistochemistry-Paraffin-NBP2-98754-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Carm1 Antibody [NBP2-54924] - Staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54924-img0001.jpg "Immunocytochemistry/Immunofluorescence: Carm1 Antibody [NBP2-54924] - Staining of human cell line MCF7 shows localization to nucleoplasm.")
![Immunocytochemistry/Immunofluorescence: Carm1 Antibody [NBP2-55993] - Staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55993-img0001.jpg "Immunocytochemistry/Immunofluorescence: Carm1 Antibody [NBP2-55993] - Staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.")
![Western Blot: CARM1 Overexpression Lysate (Adult Normal) [NBL1-08698] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CARM1.](http://images.novusbio.com/fullsize/CARM1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08698-img0002.jpg "Western Blot: CARM1 Overexpression Lysate (Adult Normal) [NBL1-08698] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CARM1.")