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![Western Blot: Brg1 Antibody [NB100-2594] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30seconds.](http://images.novusbio.com/fullsize/Brg1-Antibody-Western-Blot-NB100-2594-img0019.jpg "Western Blot: Brg1 Antibody [NB100-2594] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30seconds.")
![Immunohistochemistry-Paraffin: Brg1 Antibody [NB100-2594] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiBRG1/SMARCA4 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-Paraffin-NB100-2594-img0017.jpg)
![Western Blot: Brg1 Antibody [NB100-2594] - Whole cell lysate (15 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Brg1-Antibody-Western-Blot-NB100-2594-img0018.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody [NB100-2594] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit antiBRG1/SMARCA4 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-Paraffin-NB100-2594-img0016.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Brg1 Antibody [NB100-2594] - Detection of human BRG1-SMARCA4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRG1-SMARCA4 antibody NB100-2594 used for IP at 3 ug per reaction. BRG1-SMARCA4 was also immunoprecipitated by rabbit anti-BRG1-SMARCA4 antibody NBP-22234. For blotting immunoprecipitated BRG1-SMARCA4, NB100-2594 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunoprecipitation-NB100-2594-img0020.jpg)
![Western Blot: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of BRG1 on different lysates using anti-BRG1 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: HepG2](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Western-Blot-NBP2-74964-img0001.jpg "Western Blot: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of BRG1 on different lysates using anti-BRG1 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: HepG2")
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Staining BRG1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74964-img0005.jpg)
![Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded mouse colon tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunohistochemistry-NBP2-74964-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74964-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Staining BRG1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74964-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Staining BRG1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74964-img0004.jpg)
![Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunohistochemistry-NBP2-74964-img0006.jpg)
![Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded human kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunohistochemistry-NBP2-74964-img0007.jpg)
![Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded human tonsil tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunohistochemistry-NBP2-74964-img0008.jpg)
![Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded mouse testis tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-BSA-Free-Immunohistochemistry-NBP2-74964-img0010.jpg)
![Western Blot: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of BRG1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Western-Blot-NBP2-67720-img0013.jpg "Western Blot: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of BRG1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunocytochemistry-Immunofluorescence-NBP2-67720-img0010.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse epididymis tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0019.jpg)
![Western Blot: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of BRG1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Western-Blot-NBP2-67720-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunocytochemistry-Immunofluorescence-NBP2-67720-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunocytochemistry-Immunofluorescence-NBP2-67720-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunocytochemistry-Immunofluorescence-NBP2-67720-img0009.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0003.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0004.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human tonsil tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0005.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse colon tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0006.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse testis tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0007.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Brg1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0012.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0014.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0015.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0016.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0017.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse kidney tissue using anti-Brg1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 2 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0018.jpg)
![Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Wild-type (WT) and Brg1 knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Western-Blot-NBP3-13537-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Wild-type (WT) and Brg1 knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brg1 protein stained by Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Immunocytochemistry-Immunofluorescence-NBP3-13537-img0004.jpg)
![Immunohistochemistry-Frozen: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: Brg1 stained by Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:250. Red: SOX2, stained by SOX2 antibody [N1C3] (NBP1-33766) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Immunohistochemistry-Frozen-NBP3-13537-img0007.jpg)
![Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Western-Blot-NBP3-13537-img0001.jpg)
![Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Western-Blot-NBP3-13537-img0005.jpg)
![Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Western-Blot-NBP3-13537-img0006.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Immunohistochemistry-Paraffin-NBP3-13537-img0002.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Immunohistochemistry-Paraffin-NBP3-13537-img0003.jpg)
![Western Blot: Brg1 Antibody [NBP2-41270] - Analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Brg1-Antibody-Western-Blot-NBP2-41270-img0001.jpg "Western Blot: Brg1 Antibody [NBP2-41270] - Analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 ug/ml.")
![Immunohistochemistry-Paraffin: Brg1 Antibody [NBP2-41270] - Mouse testis tissue with SMARCA4 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-Paraffin-NBP2-41270-img0005.jpg)
![Immunohistochemistry: Brg1 Antibody [NBP2-41270] - Rat brain tissue with BRG1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-NBP2-41270-img0003.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody [NBP2-41270] - Rat brain tissue with BRG1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-Paraffin-NBP2-41270-img0002.jpg)
![Immunohistochemistry: Brg1 Antibody [NBP2-41270] - Mouse testis tissue with SMARCA4 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunofluorescence-NBP2-41270-img0004.jpg)
![Immunohistochemistry: Brg1 Antibody - BSA Free [NBP2-41270] - Immunohistochemistry of Brg1 in rat brain tissue with Brg1 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41270_rabbit-polyclonal-brg1-antibody-2711202420141992.jpg)
![Western Blot: Brg1 Antibody - BSA Free [NBP2-41270] - Western blot analysis of Brg1 in SK-N-SH cell lysate with Brg1 antibody at 1 u/ml.](http://images.novusbio.com/fullsize/nbp2-41270_rabbit-polyclonal-brg1-antibody-2711202419463480.gif)
![Immunohistochemistry: Brg1 Antibody - BSA Free [NBP2-41270] - Immunohistochemistry of Brg1 in rat brain tissue with Brg1 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41270_rabbit-polyclonal-brg1-antibody-271120242013186.jpg)
![Western Blot: Brg1 Antibody - BSA Free [NBP2-41270] - Western blot analysis of Brg1 in SK-N-SH cell lysate with Brg1 antibody at 1 u/ml.](http://images.novusbio.com/fullsize/nbp2-41270_rabbit-polyclonal-brg1-antibody-271120241954158.gif)
![Immunohistochemistry: Brg1 Antibody - BSA Free [NBP2-41270] - Immunohistochemistry of Brg1 in rat brain tissue with Brg1 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41270_rabbit-polyclonal-brg1-antibody-271120241928856.jpg)
![Immunohistochemistry: Brg1 Antibody - BSA Free [NBP2-41270] - Immunohistochemistry of Brg1 in rat brain tissue with Brg1 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41270_rabbit-polyclonal-brg1-antibody-27112024201313.jpg)
![Western Blot: Brg1 Antibody (BLR106H) [NBP3-14696] - Whole cell lysate (50 ug) from Jurkat, K-562, HEK293T, MCF-7, HeLa, LNCaP, U2OS, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1recombinant monoclonal antibody [BLR106H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit antiCOPB2 antibody.](http://images.novusbio.com/fullsize/Brg1-Antibody-BLR106H-Western-Blot-NBP3-14696-img0004.jpg "Western Blot: Brg1 Antibody (BLR106H) [NBP3-14696] - Whole cell lysate (50 ug) from Jurkat, K-562, HEK293T, MCF-7, HeLa, LNCaP, U2OS, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1recombinant monoclonal antibody [BLR106H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit antiCOPB2 antibody.")
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (BLR106H) [NBP3-14696] - Rabbit anti-BRG1 recombinant monoclonal antibody [BLR106H]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Brg1-Antibody-BLR106H-Immunocytochemistry-Immunofluorescence-NBP3-14696-img0005.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (BLR106H) [NBP3-14696] - Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody [BLR106H] Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Brg1-Antibody-BLR106H-Immunohistochemistry-Paraffin-NBP3-14696-img0002.jpg)
![Western Blot: Brg1 Antibody (BLR106H) [NBP3-14696] - Whole cell lysate (50 ug) from mIMCD-3 and A20 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody [BLR106H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit antiCOPB2 antibody.](http://images.novusbio.com/fullsize/Brg1-Antibody-BLR106H-Western-Blot-NBP3-14696-img0003.jpg)
![Immunoprecipitation: Brg1 Antibody [NBP3-14696] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-BRG1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. BRG1 was alsoimmunoprecipitated by rabbit anti-BRG1 antibody. For blotting immunoprecipitated BRG1, was used at 1:1000. Chemiluminescence withan exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunoprecipitation-NBP3-14696-img0001.jpg)
![Western Blot: Brg1 Antibody (4N9C4) [NBP3-15773] - Western blot analysis of extracts of various cell lines, using Brg1 antibody (NBP3-15773) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Brg1-Antibody-4N9C4-Western-Blot-NBP3-15773-img0006.jpg "Western Blot: Brg1 Antibody (4N9C4) [NBP3-15773] - Western blot analysis of extracts of various cell lines, using Brg1 antibody (NBP3-15773) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (4N9C4) [NBP3-15773] - Immunofluorescence analysis of NIH-3T3 cells using Brg1 antibody (NBP3-15773). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Brg1-Antibody-4N9C4-Immunocytochemistry-Immunofluorescence-NBP3-15773-img0005.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (4N9C4) [NBP3-15773] - Immunohistochemistry of paraffin-embedded mouse spleen using Brg1 antibody (NBP3-15773) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Brg1-Antibody-4N9C4-Immunohistochemistry-Paraffin-NBP3-15773-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (4N9C4) [NBP3-15773] - Immunofluorescence analysis of C6 cells using Brg1 antibody (NBP3-15773). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Brg1-Antibody-4N9C4-Immunocytochemistry-Immunofluorescence-NBP3-15773-img0004.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (4N9C4) [NBP3-15773] - Immunohistochemistry of paraffin-embedded human oophoroma using Brg1 antibody (NBP3-15773) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Brg1-Antibody-4N9C4-Immunohistochemistry-Paraffin-NBP3-15773-img0001.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (4N9C4) [NBP3-15773] - Immunohistochemistry of paraffin-embedded rat brain using Brg1 antibody (NBP3-15773) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Brg1-Antibody-4N9C4-Immunohistochemistry-Paraffin-NBP3-15773-img0002.jpg)
![Western Blot: Brg1 Antibody [NBP2-22234] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Brg1-Antibody-Western-Blot-NBP2-22234-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Brg1 Antibody [NBP2-22234] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Brg1 Antibody [NBP2-22234] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- BRG1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-NBP2-22234-img0014.jpg)
![Immunohistochemistry: Brg1 Antibody [NBP2-22234] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- BRG1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-NBP2-22234-img0013.jpg)
![Immunoprecipitation: Brg1 Antibody [NBP2-22234] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22234 used for IP at 6 ug/mg lysate. BRG1 was also immunoprecipitated by rabbit anti-BRG1 antibody NB100-2594. For blotting immunoprecipitated BRG1, NBP2-22234 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunoprecipitation-NBP2-22234-img0002.jpg)
![Immunohistochemistry: Brg1 Antibody (22F3) [NBP3-26314] - Image of Brg1 Antibody (22F3) diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.44% DAB.](http://images.novusbio.com/fullsize/nbp3-26314_rabbit-brg1-mab-22f3-262202414471597.jpg "Immunohistochemistry: Brg1 Antibody (22F3) [NBP3-26314] - Image of Brg1 Antibody (22F3) diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.44% DAB.")
![Immunohistochemistry: Brg1 Antibody (22F3) [NBP3-26314] - Image of Brg1 Antibody (22F3) diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.44% DAB.](http://images.novusbio.com/fullsize/nbp3-26314_rabbit-brg1-mab-22f3-2722024831379.jpg)
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (22F3) [NBP3-26314] - Staining of Hela with Brg1 Antibody (22F3) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 511-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26314_rabbit-brg1-mab-22f3-2822024131225.jpg)
![Flow Cytometry: Brg1 Antibody (22F3) [NBP3-26314] - Overlay Peak curve showing Hela cells stained with Brg1 Antibody (22F3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26314_rabbit-brg1-mab-22f3-282202414183640.jpg)