| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-201SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF886-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Unpurified | NB500-205 | |||
| Unpurified | NB500-205SS | |||
| BSA Free | NB500-238SS | |||
| Azide and BSA Free | NBP2-80969 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59503SS | |||
| Azide and BSA Free | NBP2-80971 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80970 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-237SS | |||
| Azide and BSA Free | NBP2-80968 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-92717SS | |||
![Dual RNAscope ISH-IHC: Survivin Antibody [NB500-201] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal [NB500-201] at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tumor cells.](http://images.novusbio.com/fullsize/Survivin-Antibody-Dual-RNAscope-ISH-IHC-NB500-201-img0026.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: Survivin Antibody [NB500-201] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal [NB500-201] at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tumor cells.")
![<b>Biological Strategies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Knockdown Validated: Survivin Antibody [NB500-201] - Western blot analysis using Survivin and flotillin antibodies was performed on lysates of DMSO- and PTX-treated MDAMB231 cells ectopically expressing either control siRNA or Survivin siRNA (panels labeled WCL), as well as on the exosomes these cells generated (panels labeled Exos). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/8/12/111) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Survivin-Antibody-Knockdown-Validated-NB500-201-img0037.jpg)
![Immunohistochemistry: Survivin Antibody [NB500-201] - Protein expression pattern of Survivin in HCC tissues and non-neoplastic liver parenchyma. IAP members immunoreactivity was estimated by tissue microarray in a subset of HCC patients (n = 40). A-D, Representative Survivin cytoplasmatic immunostaining in a tumor core (A), in a tumor proximal to cirrhosis (C, N: cirrhosis, K: HCC), and in adjacent and long-distance non-neoplastic parenchyma (B and D, respectively). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19397802) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-NB500-201-img0038.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Survivin Antibody [NB500-201] - Anti-tumor effects and functional evidence of sCA-survivin-siRNA in HCT116 and HT29 solid tumor models. Immunostaining of survivin in the tumor tissues on day 19 using [NB500-201]. Scale bar, 50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116022) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Survivin-Antibody-Knockdown-Validated-NB500-201-img0033.jpg)
![Immunocytochemistry/Immunofluorescence: Survivin Antibody [NB500-201] - Analysis using the HRP conjugate of [NB500-201]. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. Survivin detection using [NB500-201].](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunocytochemistry-Immunofluorescence-NB500-201-img0018.jpg)
![Western Blot: Survivin Antibody [NB500-201] - Analysis of 30ug of HeLa whole cell lysate [NB800-PC1] using rabbit polyclonal [NB500-201] at 1ug/ml. Detection was performed using ECL method with 1 minute exposure. Band detected at higher molecular weight than the predicted MW (16 kDa).](http://images.novusbio.com/fullsize/Survivin-Antibody-Western-Blot-NB500-201-img0017.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NB500-201] - Immunohistochemical staining of Survivin in human rectal cancer using [NB500-201] and DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NB500-201-img0008.jpg)
![Flow Cytometry: Survivin Antibody [NB500-201] - An intracellular stain was performed on HeLa cells with [NB500-201] and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/Survivin-Antibody-Flow-Cytometry-NB500-201-img0025.jpg)
![Western Blot: Survivin Antibody [NB500-201] - Western blot analysis using Survivin Antibody [NB500-201]. Effects of siRNA transfection on the expression of Bcl-xL and survivin. Bcl-xL and survivin protein content detected by Western Blotting 48 h after transfection. Bcl-xL and survivin levels are shown normalized to the reference protein beta-actin and relative to the ns-si control. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/14/6/12297) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Survivin-Antibody-Western-Blot-NB500-201-img0032.jpg)
![Western Blot: Survivin Antibody [NB500-201] - Analysis of Survivin in human hepatocytes from cancer patient (left) and HeLa cell lysate (right) using [NB500-201]. Image from verified customer review. Note: bands detected at higher molecular weight than predicted (16 kDa)](http://images.novusbio.com/fullsize/Survivin-Antibody-Western-Blot-NB500-201-img0013.jpg)
![Western Blot: Survivin Antibody [NB500-201] - Western blot analysis using [NB500-201]. Survivin protein is degraded by ubiquitin proteasome in serum-free and serum-containing media. Western blots and protein quantitation graphs showing that addition of MG-132 proteasome inhibitor (10 uM) extended survivin protein half-life in serum-free and serum-containing media. One representative western blot out of triplicate experiments was shown for each treatment and condition. *Indicates the time at which survivin protein is half of the amount at 0 hours (half-life), P 100. ** p-value 100. ** p-value < .01 using student t-test. H. PFGE analysis of EBV episomes & linears at 7 days post-transduction with lentivirus shRNA for shControl or shSurvivin in MUTU I or RAJI cell lines. Human alpha satellite DNA is used for DNA loading control Western blot of Survivin & Actin are shown below for each cell at 7 days post-transduction. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28077791), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-201_rabbit-polyclonal-survivin-antibody-310202416171444.jpg)
![Western Blot: Survivin Antibody - BSA Free [NB500-201] - The p53-p21 axis facilitates downregulation of survivin via cell cycle(A) HCT116 wild typeand p21-deficient (p21-/-) cells were treated with 5 μM L-OHP for 24 hours. Whole cell lysates were analyzed with antibodies against p53, p21, & survivin; vinculin serves as loading control. (B) Cell cycle distribution was analyzed after 24 hours treatment by flow cytometry analysis (n = 3). (C) To induce p21, RKO p21ind cells were treated with 3 nM Muristerone A for 24 hours & tested for the levels of p21 & survivin; vinculin, loading control. (D)BIRC5 mRNA levels were analyzed by quantitative real-time PCR after 24 hours treatment with MurA in RKO p21ind cells (n = 3). (E) Cell cycle distribution was measured by flow cytometry analyses of cellular DNA content (n = 3). (F) Scheme summarizing the supposed mechanisms of survivin regulation after L-OHP & CPT-11 treatment. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29963241), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-201_rabbit-polyclonal-survivin-antibody-310202416182643.jpg)
![Western Blot: Survivin Antibody - BSA Free [NB500-201] - Apoptosis & survival signaling after L-OHP & CPT-11(A) Western blot analysis using antibodies against p53 & pro-apoptotic BAX & PIG-3 after treatment with 5 μM L-OHP or 10 μM CPT-11. (B) Immunodetection of NF-kappa B p65, RELB & anti-apoptotic survivin, XIAP, BCL-XL & MCL1; vinculin serves as loading control. (C) Effects of increasing doses L-OHP & CPT-11 on caspase-3 & PARP1 cleavage after 24 hours treatment; alpha -tubulin serves as loading control. (D) Cells were treated with a combination of L-OHP & the caspase-inhibitor Z-VAD-FMK (50 μM). Immunodetection of survivin, p53 & full-length caspase-3 was conducted. Detection of apoptosis was determined by cleavage products of caspase-3 & PARP1; beta -actin serves as loading control. Please note: Figure 4A & 4B, as well as Supplementary Figure 2A show signals acquired by different detection methods, but originate from the same Western blots. This is due to a switch in the immunoblot chemiluminescence detection system from X-ray films (darker background) to a CCD camera system (Fusion Solo S, Vilber Lourmat; lighter background). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29963241), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-201_rabbit-polyclonal-survivin-antibody-310202416175291.jpg)
![Western Blot: Survivin Antibody - BSA Free [NB500-201] - Anti-tumor effects & functional evidence of sCA-survivin-siRNA in HCT116 & HT29 solid tumor models.(A) For western blot analysis, HCT116 cells were seeded into 6-well plates & transfected with 100 pmol/well of either control or survivin siRNA by Lp (Lipofectamine 2000) or sCA. Actin blots served as loading controls. (B) For proliferation assays, cells were uniformly seeded into 96-well plates (1 × 104 cells/well), & 5 pmol/well siRNA was used. Cell viability was examined at 48 & 72 h by WST-8 assay. Data represent mean ± SEM. *P = 0.0294, **P = 0.0304 (n = 4, Wilcoxon rank test). (C) In vivo tumor growth. Each vehicle, carrying 15 μg of control siRNA or survivin siRNA, was administered by intravenous injection to mice with HCT116 tumors. Data represent the mean ± SEM (n = 10 tumors, Wilcoxon rank test). (D) Immunostaining of survivin in the tumor tissues on day 19. Scale bar, 50 μm. (E) In vivo live imaging of sCA-siRNA (6-FAM labeled) in HT29 tumor by multiphoton microscopy at 90 min. (F) Fluorescent detection of naked-siRNA (6-FAM labeled) or sCA-siRNA (6-FAM labeled) in the HT29 tumor at 4 h. Green: 6-FAM labeled siRNA; Red: microvasculature; Blue: DAPI stained nuclei. Scale bar, 50 μm. (G) Mice were administered with 40 μg of naked-survivin-siRNA or sCA-survivin-siRNA on days 0, 1, & 2. Tumors were removed on day 3, & western blot analysis for survivin was performed. (H) Many tumor cells treated with sCA-survivin-siRNA (6-FAM labeled) had condensed nuclei & were positive for TUNEL assay. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25738937), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-201_rabbit-polyclonal-survivin-antibody-310202416175225.jpg)
![Western Blot: Survivin Antibody - BSA Free [NB500-201] - Survivin is essential for the salinomycin-induced apoptosis in UM cells. a UM cells were treated with salinomycin for 24 h & the protein levels of apoptosis-related proteins were detected by Western blot. b & c UM cells were transduced with lentiviral pTSB-Survivin cDNA (b), Survivin-shRNA constructs (c), or their corresponding empty vectors, & then incubated in the presence of puromycin (1 μg/mL) for 5 days to reach stable clones. Such survivin-manipulated cells were then exposed to salinomycin for 24 h, & subjected to trypan blue exclusion assay (left) & Western blot assay (right). Data represent mean ± SD. ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001, Student’s t test. d qRT-PCR analysis of BIRC5 mRNA level was done in the 92.1 & Mel270 cells treated with salinomycin for 24 h. **, P < 0.01; ***, P < 0.001, one-way ANOVA, post hoc comparisons, Tukey’s test Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31718679), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-201_rabbit-polyclonal-survivin-antibody-310202416161820.jpg)
![Immunocytochemistry/ Immunofluorescence: Survivin Antibody - BSA Free [NB500-201] - Anti-tumor effects & functional evidence of sCA-survivin-siRNA in HCT116 & HT29 solid tumor models.(A) For western blot analysis, HCT116 cells were seeded into 6-well plates & transfected with 100 pmol/well of either control or survivin siRNA by Lp (Lipofectamine 2000) or sCA. Actin blots served as loading controls. (B) For proliferation assays, cells were uniformly seeded into 96-well plates (1 × 104 cells/well), & 5 pmol/well siRNA was used. Cell viability was examined at 48 & 72 h by WST-8 assay. Data represent mean ± SEM. *P = 0.0294, **P = 0.0304 (n = 4, Wilcoxon rank test). (C) In vivo tumor growth. Each vehicle, carrying 15 μg of control siRNA or survivin siRNA, was administered by intravenous injection to mice with HCT116 tumors. Data represent the mean ± SEM (n = 10 tumors, Wilcoxon rank test). (D) Immunostaining of survivin in the tumor tissues on day 19. Scale bar, 50 μm. (E) In vivo live imaging of sCA-siRNA (6-FAM labeled) in HT29 tumor by multiphoton microscopy at 90 min. (F) Fluorescent detection of naked-siRNA (6-FAM labeled) or sCA-siRNA (6-FAM labeled) in the HT29 tumor at 4 h. Green: 6-FAM labeled siRNA; Red: microvasculature; Blue: DAPI stained nuclei. Scale bar, 50 μm. (G) Mice were administered with 40 μg of naked-survivin-siRNA or sCA-survivin-siRNA on days 0, 1, & 2. Tumors were removed on day 3, & western blot analysis for survivin was performed. (H) Many tumor cells treated with sCA-survivin-siRNA (6-FAM labeled) had condensed nuclei & were positive for TUNEL assay. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25738937), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-201_rabbit-polyclonal-survivin-antibody-310202416182634.jpg)
![Immunohistochemistry: Survivin Antibody (60.11) [NB500-238] - Low-power image of human high-grade chondrosarcoma displays strong cellular expression of survivin protein (A). High-power magnification reveals the predominantly cytoplasmic staining, although strong nuclear signals are detectable (B). Other specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining (C and D). For A and B the polyclonal rabbit anti-survivin antibody [AF886] was used. For C and D the monoclonal mouse Survivin Antibody (60.11) [NB500-238] was used. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Survivin-Antibody-60-11-Immunohistochemistry-NB500-238-img0019.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry: Survivin Antibody (60.11) [NB500-238] - Low-power image of human high-grade chondrosarcoma displays strong cellular expression of survivin protein (A). High-power magnification reveals the predominantly cytoplasmic staining, although strong nuclear signals are detectable (B). Other specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining (C and D). For A and B the polyclonal rabbit anti-survivin antibody [AF886] was used. For C and D the monoclonal mouse Survivin Antibody (60.11) [NB500-238] was used. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license.")
![<b>Independent Antibodies Validation. </b>Flow (Intracellular): Survivin Antibody (60.11) [NB500-238] - An intracellular stain was performed on A549 cells with Survivin Antibody (60.11) [NB500-238] and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image using the Unpurified form of this antibody.](http://images.novusbio.com/fullsize/Survivin-Antibody-60-11-Flow-Intracellular-NB500-238-img0015.jpg)
![Western Blot: Survivin Antibody (60.11) [NB500-238] - Survivin detection in 30ug of HeLa whole cell extract using [NB500-238] at 1ug/ml. Detected at predicted molecular weight of 16 kDa.](http://images.novusbio.com/fullsize/Survivin-Antibody-60-11-Western-Blot-NB500-238-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Survivin Antibody (60.11) [NB500-238] - Knockdown Validation of Survivin Antibody (60.11) [NB500-238]. Suppression of survivin expression by transfection of siRNA. RNA interference was performed in SW1353 and Hs 819.T, either for GFP as control or for survivin. A: A pronounced decrease of survivin protein levels was measured by immunoblotting in SW1353 and Hs819.T. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Survivin-Antibody-60-11-Knockdown-Validated-NB500-238-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Survivin Antibody (60.11) [NB500-238] - HeLa cells stained NB500-205 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue). Image using the Unpurified format of this antibody.](http://images.novusbio.com/fullsize/Survivin-Antibody-60-11-Immunocytochemistry-Immunofluorescence-NB500-238-img0021.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody (60.11) [NB500-238] - Staining of ovary cancer. Image using the Unpurified format of this antibody.](http://images.novusbio.com/fullsize/Survivin-Antibody-60-11-Immunohistochemistry-Paraffin-NB500-238-img0022.jpg)
![Flow Cytometry: Survivin Antibody (60.11) [NB500-238] - An intracellular stain was performed on U2OS cells with Survivin Antibody (60.11) [NB500-238AF700] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Survivin-Antibody-60-11-Flow-Cytometry-NB500-238-img0017.jpg)
![Flow (Intracellular): Survivin Antibody (60.11) [NB500-238] - An intracellular stain was performed on Daudi cells with Survivin Antibody (60.11)[NB500-238] (blue) and a matched isotype control, Mouse IgG2a Kappa Light Chain Isotype Control (MG2a-53) [NB600-986] (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Survivin-Antibody-60-11-Flow-Intracellular-NB500-238-img0014.jpg)
![Western Blot: Survivin Antibody (60.11) - BSA Free [NB500-238] - Binding of antibody (60.11) used in imaging flow cytometry to full-length survivin protein & to survivin vaccine peptide aa53-67/M57 & wild type peptide aa53-67 Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.21773), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-238_mouse-monoclonal-survivin-antibody-60-11-31020241539726.jpg)
![Western Blot: Survivin Antibody (60.11) - BSA Free [NB500-238] - CD133+ cells are more resistant to chemotherapeutic agents. STA-ET-8.2 cells were FACS-sorted & CD133+ & CD133- fractions treated with increasing concentrations of (A) Doxorubicin (Doxo), (B) Etoposide (Etop), (C) Vincristine (Vinc) or (D) a combination of all three drugs at high (Doxo 10 μM; Etop 10 ug/ml; Vinc 100 ng/ml), medium (Doxo 2.5 μM; 20 Etop 2.5 ug/ml; Vinc 25 ng/ml) & low (Doxo 0.5 μM; Etop 0.5 ug/ml; Vinc5 ng/ml) concentrations. Viability was assessed by MTS assay after 96 hrs (*p < 0.05) & CD133+ cells displayed increased drug resistance. (E): CD133+ & CD133- TC-71 cells are equally sensitive to Doxo, Etop, & Vinc. (F): Western blot reveals no difference in apoptosis & drug resistance protein expression between unsorted & CD133-sorted STA-ET-8.2 cell fractions. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20346143), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-238_mouse-monoclonal-survivin-antibody-60-11-310202416163762.jpg)
![Simple Western: Survivin Antibody (1277A) [NBP2-59503] - Lane view shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 23 kDa (as indicated) using 10 ug/mL of Rabbit Anti-Human Monoclonal Survivin Antibody [NBP2-59503]. This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.](http://images.novusbio.com/fullsize/Survivin-Antibody-1277A-Simple-Western-NBP2-59503-img0002.jpg "Simple Western: Survivin Antibody (1277A) [NBP2-59503] - Lane view shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 23 kDa (as indicated) using 10 ug/mL of Rabbit Anti-Human Monoclonal Survivin Antibody [NBP2-59503]. This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.")
![Immunohistochemistry-Paraffin: Survivin Antibody (1277A) [NBP2-59503] - Analysis of a formalin-fixed paraffin-embedded (FFPE) human prostate cancer tissue with rabbit monoclonal Survivin Antibody [NBP2-59503] at 2 ug/ml concentration. The staining was developed with HRP-DAB detection method and the sections were counterstained using hematoxylin. This Survivin antibody generated an expected nuclear cytoplasmic immunostaining in a subset of cancer cells, which are potentially the proliferating cells.](http://images.novusbio.com/fullsize/Survivin-Antibody-1277A-Immunohistochemistry-Paraffin-NBP2-59503-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Survivin Antibody (1277A) [NBP2-59503] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with Survivin Antibody (1277A) [NBP2-59503] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Survivin-Antibody-1277A-Immunocytochemistry-Immunofluorescence-NBP2-59503-img0005.jpg)
![Western Blot: Survivin Antibody (1277A) [NBP2-59503] - Total protein from human HeLa and A431 cell lines was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um Polyvinylidene fluoride (PVDF) membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml [NBP2-59503] in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note: predicted molecular weight is 16 kDa.](http://images.novusbio.com/fullsize/Survivin-Antibody-1277A-Western-Blot-NBP2-59503-img0004.jpg)
![Flow (Intracellular): Survivin Antibody (1277A) [NBP2-59503] - An intracellular stain was performed on HeLa Cells with Survivin Antibody (1277A) [NBP2-59503] (blue) and a matched isotype control [MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody [F0111, R&D Systems].](http://images.novusbio.com/fullsize/Survivin-Antibody-1277A-Flow-Intracellular-NBP2-59503-img0003.jpg)
![Immunohistochemistry: Survivin Antibody (8E2) [NB500-644] - Immunohistochemical analysis of Survivin in human breast cancer tissue using Survivin Antibody (8E2) [NB500-644] and DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Immunohistochemistry-NB500-644-img0005.jpg "Immunohistochemistry: Survivin Antibody (8E2) [NB500-644] - Immunohistochemical analysis of Survivin in human breast cancer tissue using Survivin Antibody (8E2) [NB500-644] and DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: Survivin Antibody (8E2) [NB500-644] - Immunocytochemical analysis using Survivin Antibody (8E2) [NB500-644] was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Immunocytochemistry-Immunofluorescence-NB500-644-img0004.jpg)
![Flow (Intracellular): Survivin Antibody (8E2) [NB500-644] - An intracellular stain was performed on Daudi cells with Survivin Antibody (8E2) [NB500-644] (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Flow-Intracellular-NB500-644-img0006.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody (32.1) [NB500-237] - Survivin expression in human chondrosarcoma. Immunohistochemistry and immunoblot for survivin (red staining) from human chondrosarcoma specimens. Specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining. Image collected and cropped by Citeab from the following publication (The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro. BMC Cancer (2011) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Survivin-Antibody-32-1-Immunohistochemistry-Paraffin-NB500-237-img0004.jpg "Immunohistochemistry-Paraffin: Survivin Antibody (32.1) [NB500-237] - Survivin expression in human chondrosarcoma. Immunohistochemistry and immunoblot for survivin (red staining) from human chondrosarcoma specimens. Specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining. Image collected and cropped by Citeab from the following publication (The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro. BMC Cancer (2011) licensed under a CC-BY license.")
![Flow Cytometry: Survivin Antibody (32.1) [NB500-237] - An intracellular stain was performed on HeLa cells with FITC-conjugated Survivin Antibody (32.1) [NB500-237F] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Survivin-Antibody-32-1-Flow-Cytometry-NB500-237-img0003.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody (32.1) [NB500-237] - Immunohistochemical staining of formalin-fixed paraffin-embedded human lung cancer tissue using Survivin Antibody (32.1) [NB500-237].](http://images.novusbio.com/fullsize/Survivin-Antibody-32-1-Immunohistochemistry-Paraffin-NB500-237-img0002.jpg)
![Western Blot: Survivin Antibody (32.1) - BSA Free [NB500-237] - Survivin expression in human chondrosarcoma. Immunohistochemistry & immunoblot for survivin (red staining) from human chondrosarcoma specimens. A: Low-power image of human high-grade chondrosarcoma displays strong cellular expression of survivin protein. B: High-power magnification reveals the predominantly cytoplasmic staining, although strong nuclear signals are detectable. C & D: Other specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining. E: Strong survivin signal in a tumor cell displaying a mitotic figure (arrow). F: To verify the expression of survivin in human chondrosarcoma, immunoblots were performed from 3 high grade chondrosarcoma lysates (Patient Nr. 5, 7, 10). As control for the correct molecular weight, in vitro-transcribed & -translated (IVTT) recombinant survivin protein, derived from the full-length human cDNA was loaded. Furthermore, lysates from adult human cartilage served as a negative control. Total protein loaded was 1 μg for recombinant human survivin, 60 μg for chondrosarcoma & cartilage lysates. For A, B & E the polyclonal rabbit anti-survivin antibody AF886 was used. For C & D the monoclonal mouse anti-survivin antibody clone 32.1 was used. Original magnifications: 200× (A & C) & 400× (B & D) & 600× (E). Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-237_mouse-monoclonal-survivin-antibody-32-1-31020241616086.jpg)
![Dual RNAscope ISH-IHC: Survivin Antibody Pack [NB100-911] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal [NB500-201] at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tumor cells.](http://images.novusbio.com/fullsize/Survivin-Antibody-Pack-RNAscope-compatible-NB100-911-img0006.jpg "Dual RNAscope ISH-IHC: Survivin Antibody Pack [NB100-911] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal [NB500-201] at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tumor cells.")
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Survivin Antibody Pack [NB100-911] - Anti-tumor effects and functional evidence of sCA-survivin-siRNA in HCT116 and HT29 solid tumor models. Immunostaining of survivin in the tumor tissues on day 19 using [NB500-201]. Scale bar, 50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116022) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Survivin-Antibody-Pack-Knockdown-Validated-NB100-911-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Survivin Antibody Pack [NB100-911] - Suppression of survivin expression by transfection of siRNA. RNA interference was performed in SW1353 and Hs 819.T, either for GFP as control or for survivin. A: A pronounced decrease of survivin protein levels was measured by immunoblotting in SW1353 and Hs819.T. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license. Survivin Antibody (60.11) [NB500-238]](http://images.novusbio.com/fullsize/Survivin-Antibody-Pack-Knockdown-Validated-NB100-911-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Survivin Antibody Pack [NB100-911] - Analysis using the HRP conjugate of [NB500-201]. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. Survivin detection using [NB500-201].](http://images.novusbio.com/fullsize/Survivin-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-911-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Survivin Antibody Pack [NB100-911] - Low-power image of human high-grade chondrosarcoma displays strong cellular expression of survivin protein (A). High-power magnification reveals the predominantly cytoplasmic staining, although strong nuclear signals are detectable (B). Other specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining (C and D). For A and B the polyclonal rabbit anti-survivin antibody [AF886] was used. For C and D the monoclonal mouse Survivin Antibody (60.11) [NB500-238] was used. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Survivin-Antibody-Pack-Immunohistochemistry-NB100-911-img0005.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody Pack [NB100-911] - Immunohistochemical staining of formalin-fixed paraffin-embedded human lung cancer tissue using Survivin Antibody (32.1) [NB500-237].](http://images.novusbio.com/fullsize/Survivin-Antibody-Pack-Immunohistochemistry-Paraffin-NB100-911-img0009.jpg)
![Western Blot: Survivin [p Thr34] Antibody [NB500-236] - Western blot analysis using [NB500-236]. Lane 1: Phosphorylated Survivin protein and Lane 2: Non-phosphorylated Survivin protein. Note: theoretical molecular weight of Survivin Antibody: 16 kDa.](http://images.novusbio.com/fullsize/Survivin-[p-Thr34]-Antibody-Western-Blot-NB500-236-img0003.jpg "Western Blot: Survivin [p Thr34] Antibody [NB500-236] - Western blot analysis using [NB500-236]. Lane 1: Phosphorylated Survivin protein and Lane 2: Non-phosphorylated Survivin protein. Note: theoretical molecular weight of Survivin Antibody: 16 kDa.")
![Immunocytochemistry/Immunofluorescence: Survivin [p Thr34] Antibody [NB500-236] - Immunocytochemical analysis using Survivin [p Thr34] Antibody [NB500-236] in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Survivin-[p-Thr34]-Antibody-Immunocytochemistry-Immunofluorescence-NB500-236-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Survivin [p Thr34] Antibody [NB500-236] - Combination therapy with TiOxNPs and IR suppressed the aggressiveness of xenografts in dissociated MIA PaCa sphere cell-bearing mice. Western blot for expression of Survivin [p Thr34] (NB500-236) in xenografts of dissociated MIA PaCa sphere-bearing cells treated with TiOxNPs and/or irradiation. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428310/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb500-236_rabbit-polyclonal-survivin-p-thr34-antibody-2552023151499.jpg)
![Western Blot: Survivin [p Thr34] Antibody [NB500-236] - Combination therapy with TiOxNPs & IR suppressed the aggressiveness of xenografts in dissociated MIA PaCa sphere cell-bearing mice. A HE staining & IHC analysis of p-H2AX, c-caspase-3, ki67, PCNA, snail/Slug, & vimentin in the indicated groups. Scale bar = 50 μm. n=5. B Western blot for expression of p-H2AX, c-caspase-3, p-survivin, snail/Slug, & vimentin in xenografts of dissociated MIA PaCa sphere-bearing cells treated with TiOxNPs and/or irradiation. C In vivo apoptosis marker TUNEL assay in xenografts of dissociated MIA PaCa sphere-bearing cells treated with TiOxNPs and/or irradiation. n=5. Data are shown as the mean ± standard deviation. ns, not significant. ****p < 0.0001. Scale bar = 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428310), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-236_rabbit-polyclonal-survivin-p-thr34-antibody-31020241539717.jpg)
![Western Blot: Survivin Antibody [NB100-56167] - Analysis of Survivin using [NB100-56167]. Survivin was detected in the two tumor cell lines, Jurkat (T cell lymphoma) and RS11846 (non-Hodgkin's lymphoma), but not in lysates from normal tissues. Survivin RP, full-length recombinant human survivin protein was used as a positive control. Bands observed slightly lower than the theoretical molecular weight, 16 kDa.](http://images.novusbio.com/fullsize/Survivin-Antibody-Western-Blot-NB100-56167-img0005.jpg "Western Blot: Survivin Antibody [NB100-56167] - Analysis of Survivin using [NB100-56167]. Survivin was detected in the two tumor cell lines, Jurkat (T cell lymphoma) and RS11846 (non-Hodgkin's lymphoma), but not in lysates from normal tissues. Survivin RP, full-length recombinant human survivin protein was used as a positive control. Bands observed slightly lower than the theoretical molecular weight, 16 kDa.")
![Western Blot: Survivin Antibody [NB100-56167] - Representative Western blots showing the IAP levels in EV derived from individual European American (EA) (N1-N5) & (N7-N9), *N6 and African American (AA) (N1-N9) patients with prostate cancer (PCa).Specific antibodies against Survivin, XIAP, cIAP-1, cIAP-2, and Lamp1 were used for the Western blotting analysis of total exosomal proteins. The blots from both patient groups were processed under identical conditions; Lamp 1 was used as loading control. (*N6, Hispanic.) (Both blots were done side by side in the same gel running and transferring apparatus, blocking, washing buffers, and antibody incubations were done in the same time, in the same incubating trays under the identical exposure to keep the consistencies). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0183122), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Survivin-Antibody-Western-Blot-NB100-56167-img0006.jpg)
![Immunohistochemistry: Survivin Antibody [NB100-56167] - Immunohistochemical analysis of Survivin in formalin-fixed paraffin-embedded human prostate and breast cancer using Survivin Antibody [NB100-56167]. A and A1, prostate cancer at low and high magnification, respectively. B and B1, breast cancer. B1 is a high magnification of an area of metastasis. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-NB100-56167-img0002.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56167] - Analysis of Survivin in a formalin-fixed paraffin-embedded human brain tissue microarray using Survivin Antibody [NB100-56167]. A. Differential expression of Survivin in brain tumors from six different patients. A1-A3, high magnification from A. A1, gemistocytic astrocytoma. A2, medulloblastoma. A3, choroid plexus papilloma. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NB100-56167-img0004.jpg)
![ELISA Survivin [Biotin]](http://images.novusbio.com/fullsize2/dyc647-2_human-total-survivin-duoset-ic-economy-pack-15-plate-elisa-169202091050.jpg "ELISA Survivin [Biotin]")
![N/A Survivin [HRP]](http://images.novusbio.com/fullsize2/DATA_Survivin_DSV00_ELISA_835.jpg "N/A Survivin [HRP]")
![N/A Survivin [HRP]](http://images.novusbio.com/fullsize2/DATA_Survivin_DSV00_ELISA_836.jpg)
![N/A Survivin [HRP]](http://images.novusbio.com/fullsize2/Survivin_DSV00_ELISA_187.jpg)
![Western Blot: Survivin [p Ser20] Antibody [NB110-92717] - Detection of Survivin [Ser20] in phosphorylated recombinant protein (+), but not in unphosphorylated protein (-) using [NB110-92717]. Band detected at slightly higher than the predicted molecular weight of 16 kDa.](http://images.novusbio.com/fullsize/Survivin-[p-Ser20]-Antibody-Western-Blot-NB110-92717-img0002.jpg "Western Blot: Survivin [p Ser20] Antibody [NB110-92717] - Detection of Survivin [Ser20] in phosphorylated recombinant protein (+), but not in unphosphorylated protein (-) using [NB110-92717]. Band detected at slightly higher than the predicted molecular weight of 16 kDa.")
![Immunocytochemistry/Immunofluorescence: Survivin [p Ser20] Antibody [NB110-92717] - Survivin [p Ser20] Antibody (NB110-92717) was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Survivin-[p-Ser20]-Antibody-Immunocytochemistry-Immunofluorescence-NB110-92717-img0004.jpg)