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![Western Blot: AIMP2 Antibody [NBP1-81575] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/AIMP2-Antibody-Western-Blot-NBP1-81575-img0006.jpg "Western Blot: AIMP2 Antibody [NBP1-81575] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: AIMP2 Antibody [NBP1-81575] - Staining of human cell line A549 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/AIMP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81575-img0008.jpg)
![Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81575] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/AIMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81575-img0012.jpg)
![Western Blot: AIMP2 Antibody [NBP1-81575] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/AIMP2-Antibody-Western-Blot-NBP1-81575-img0005.jpg)
![Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81575] - Staining of human prostate shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/AIMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81575-img0009.jpg)
![Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81575] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/AIMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81575-img0010.jpg)
![Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81575] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/AIMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81575-img0011.jpg)
![Western Blot: AIMP2 Antibody [NBP2-81885] - Analysis of AIMP2 in HeLa cell lysate with AIMP2 antibody at 1 ug/ml in (A) the absence and (B) presence of blocking peptide.](http://images.novusbio.com/fullsize/AIMP2-Antibody-Western-Blot-NBP2-81885-img0001.jpg "Western Blot: AIMP2 Antibody [NBP2-81885] - Analysis of AIMP2 in HeLa cell lysate with AIMP2 antibody at 1 ug/ml in (A) the absence and (B) presence of blocking peptide.")
![Immunocytochemistry/Immunofluorescence: AIMP2 Antibody [NBP2-81885] - Analysis of AIMP2 in rat small intestine tissue with AIMP2 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/AIMP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-81885-img0003.jpg)
![Immunohistochemistry: AIMP2 Antibody [NBP2-81885] - Analysis of AIMP2 in rat small intestine tissue with AIMP2 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/AIMP2-Antibody-Immunohistochemistry-NBP2-81885-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: AIMP2 Antibody - BSA Free [NBP2-81885] - Immunofluorescence of AIMP2 in rat small intestine tissue with AIMP2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-81885_rabbit-polyclonal-aimp2-antibody-271120242092092.jpg)
![Immunohistochemistry: AIMP2 Antibody - BSA Free [NBP2-81885] - Immunohistochemistry of AIMP2 in rat small intestine tissue with AIMP2 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp2-81885_rabbit-polyclonal-aimp2-antibody-271120241937766.jpg)
![Western Blot: AIMP2 Antibody - BSA Free [NBP2-81885] - Western blot analysis of AIMP2 in HeLa cell lysate with AIMP2 antibody at 1 u/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp2-81885_rabbit-polyclonal-aimp2-antibody-2711202419432297.gif)
![Western Blot: AIMP2 Antibody - BSA Free [NBP2-81885] - Western blot analysis of AIMP2 in HeLa cell lysate with AIMP2 antibody at 1 u/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp2-81885_rabbit-polyclonal-aimp2-antibody-2711202420333824.gif)
![Immunohistochemistry: AIMP2 Antibody - BSA Free [NBP2-81885] - Immunohistochemistry of AIMP2 in rat small intestine tissue with AIMP2 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp2-81885_rabbit-polyclonal-aimp2-antibody-2711202420405552.jpg)
![Immunocytochemistry/ Immunofluorescence: AIMP2 Antibody - BSA Free [NBP2-81885] - Immunofluorescence of AIMP2 in rat small intestine tissue with AIMP2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-81885_rabbit-polyclonal-aimp2-antibody-2711202419313219.jpg)
![Immunohistochemistry: AIMP2 Antibody (PSH0-75) [NBP3-32011] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-AIMP2 antibody (NBP3-32011) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32011) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32011_rabbit-aimp2-mab-psh0-75-298202414262656.jpg "Immunohistochemistry: AIMP2 Antibody (PSH0-75) [NBP3-32011] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-AIMP2 antibody (NBP3-32011) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32011) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: AIMP2 Antibody (PSH0-75) [NBP3-32011] - Immunocytochemistry analysis of SH-SY5Y cells labeling AIMP2 with Rabbit anti-AIMP2 antibody (NBP3-32011) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-AIMP2 antibody (NBP3-32011) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32011_rabbit-aimp2-mab-psh0-75-29202410334045.jpg)
![Flow Cytometry: AIMP2 Antibody (PSH0-75) [NBP3-32011] - Flow cytometric analysis of SH-SY5Y cells labeling AIMP2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32011, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32011_rabbit-aimp2-mab-psh0-75-392024884641.jpg)
![Western Blot: AIMP2 Antibody (PSH0-75) [NBP3-32011] - Western blot analysis of AIMP2 on different lysates with Rabbit anti-AIMP2 antibody (NBP3-32011) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: HepG2 cell lysate Lane 6: HT-29 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse kidney tissue lysate (40 ug/Lane) Lane 9: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32011) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32011_rabbit-aimp2-mab-psh0-75-3920248485025.jpg)
![Western Blot: AIMP2 Antibody [NBP2-27094] - Analysis of AIMP2 using AIMP2 antibody. Human spleen tissue lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of AIMP2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/AIMP2-Antibody-Western-Blot-NBP2-27094-img0001.jpg "Western Blot: AIMP2 Antibody [NBP2-27094] - Analysis of AIMP2 using AIMP2 antibody. Human spleen tissue lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of AIMP2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81574] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/AIMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81574-img0004.jpg "Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81574] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.")
![Western Blot: AIMP2 Antibody [NBP2-86968] - Host: Rabbit. Target Name: AIMP2. Sample Tissue: Human U937 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/AIMP2-Antibody-Western-Blot-NBP2-86968-img0001.jpg "Western Blot: AIMP2 Antibody [NBP2-86968] - Host: Rabbit. Target Name: AIMP2. Sample Tissue: Human U937 Whole Cell lysates. Antibody Dilution: 1ug/ml")
![Western Blot: AIMP2 Overexpression Lysate (Adult Normal) [NBL1-12113] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AIMP2.](http://images.novusbio.com/fullsize/AIMP2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12113-img0002.jpg "Western Blot: AIMP2 Overexpression Lysate (Adult Normal) [NBL1-12113] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AIMP2.")