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![Western Blot: NFYA Antibody [NBP2-19533] - NF-YA is essential for lincRNA-p21 regulation by mutant P53 but not wild-type p53. The binding of p53 to NF-YA in HN6, Cal27 and HN30 cells was determined by coimmunoprecipitation. *: P < 0.05; **: P < 0.01 Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/s12943-019-0993-3) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NBP2-19533-img0008.jpg "Western Blot: NFYA Antibody [NBP2-19533] - NF-YA is essential for lincRNA-p21 regulation by mutant P53 but not wild-type p53. The binding of p53 to NF-YA in HN6, Cal27 and HN30 cells was determined by coimmunoprecipitation. *: P < 0.05; **: P < 0.01 Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/s12943-019-0993-3) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: NFYA Antibody [NBP2-19533] - NFYA antibody detects NFYA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFYA stained by NFYA antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.](http://images.novusbio.com/fullsize/NFYA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19533-img0006.jpg)
![Immunohistochemistry-Paraffin: NFYA Antibody [NBP2-19533] - Immunohistochemical analysis of paraffin-embedded D54 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/NFYA-Antibody-Immunohistochemistry-Paraffin-NBP2-19533-img0003.jpg)
![Western Blot: NFYA Antibody [NBP2-19533] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 10% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NBP2-19533-img0001.jpg)
![Western Blot: NFYA Antibody [NBP2-19533] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NBP2-19533-img0002.jpg)
![Western Blot: NFYA Antibody [NBP2-19533] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NFYA antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NBP2-19533-img0005.jpg)
![Western Blot: NFYA Antibody [NBP2-19533] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NFYA antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NBP2-19533-img0007.jpg)
![Western Blot: NFYA Antibody [NB600-481] - Lane 1: CHO-7 cells were cultured in the absence (I) of cholesterol. Lane 2: CHO-7 cells were cultured in the presence (S) of cholesterol. Load: 35 ug per lane. Primary antibody: NF-YA antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: NF-YA recognized as a doublet of 42 kDa in size. Other band(s): none.](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NB600-481-img0007.jpg "Western Blot: NFYA Antibody [NB600-481] - Lane 1: CHO-7 cells were cultured in the absence (I) of cholesterol. Lane 2: CHO-7 cells were cultured in the presence (S) of cholesterol. Load: 35 ug per lane. Primary antibody: NF-YA antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: NF-YA recognized as a doublet of 42 kDa in size. Other band(s): none.")
![Immunohistochemistry: NFYA Antibody [NB600-481] - Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: NF-Y antibody at 10ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: NF-Y is nuclear. Staining: NF-Y subunit A as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/NFYA-Antibody-Immunohistochemistry-NB600-481-img0002.jpg)
![Western Blot of Rabbit Anti-NF-Y (A Subunit) Antibody. Lane 1: CHO-7 cells were cultured in the absence (I) of cholesterol. Lane 2: CHO-7 cells were cultured in the presence (S) of cholesterol. Load: 35 ug per lane. Primary antibody: NF-YA antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: NF-YA recognized as a doublet of ~ 42 kDa in size. Other band(s): none.](http://images.novusbio.com/fullsize/nb600-481_rabbit-polyclonal-nfya-antibody-25520231310519.jpg)
![Western Blot: NFYA Antibody [NBP2-48977] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NBP2-48977-img0002.jpg "Western Blot: NFYA Antibody [NBP2-48977] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: NFYA Antibody [NBP2-48977] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NFYA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48977-img0003.jpg)
![Immunohistochemistry-Paraffin: NFYA Antibody [NBP2-48977] - Staining of human stomach, lower shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NFYA-Antibody-Immunohistochemistry-NBP2-48977-img0001.jpg)
![Western Blot: NFYA Antibody [NBP2-87908] - WB Suggested Anti-NFYA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysateNFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NBP2-87908-img0003.jpg "Western Blot: NFYA Antibody [NBP2-87908] - WB Suggested Anti-NFYA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysateNFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells")
![Immunohistochemistry: NFYA Antibody [NBP2-87908] - HumanMuscle](http://images.novusbio.com/fullsize/NFYA-Antibody-Immunohistochemistry-NBP2-87908-img0002.jpg)
![Immunohistochemistry: NFYA Antibody [NBP2-87908] - Human Liver](http://images.novusbio.com/fullsize/NFYA-Antibody-Immunohistochemistry-NBP2-87908-img0001.jpg)
![Western Blot: NFYA Antibody (SR1622) [NBP3-22339] - Western blot analysis using NBP3-22339 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-22339_rabbit-nfya-mab-sr1622-57202317161813.jpg "Western Blot: NFYA Antibody (SR1622) [NBP3-22339] - Western blot analysis using NBP3-22339 in 293T cell lysate.")
![Western Blot: NFYA Antibody [NBP2-87907] - WB Suggested Anti-NFYA Antibody Titration: 5.0-8.0ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysateNFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NBP2-87907-img0002.jpg "Western Blot: NFYA Antibody [NBP2-87907] - WB Suggested Anti-NFYA Antibody Titration: 5.0-8.0ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysateNFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells")
![Immunohistochemistry: NFYA Antibody [NBP2-87907] - Human Liver](http://images.novusbio.com/fullsize/NFYA-Antibody-Immunohistochemistry-NBP2-87907-img0001.jpg)
![Western Blot: NFYA Overexpression Lysate (Adult Normal) [NBL1-13627] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFYA.](http://images.novusbio.com/fullsize/NFYA-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13627-img0002.jpg "Western Blot: NFYA Overexpression Lysate (Adult Normal) [NBL1-13627] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFYA.")
![Western Blot: NFYA Overexpression Lysate (Adult Normal) [NBL1-13628] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFYA.](http://images.novusbio.com/fullsize/NFYA-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13628-img0002.jpg "Western Blot: NFYA Overexpression Lysate (Adult Normal) [NBL1-13628] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFYA.")
![Western Blot: NFYA Antibody [NBP2-93683] - Analysis of extracts of various cell lines, using NFYA at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NBP2-93683-img0001.jpg "Western Blot: NFYA Antibody [NBP2-93683] - Analysis of extracts of various cell lines, using NFYA at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")