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![Western Blot: SMCX Antibody [NB100-55327] - Detection of Human and Mouse Jarid1C by Western Blot. Samples: Whole cell lysate (50, 15 ug) from HeLa and (50 ug) from 293T, Jurkat, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Jarid1C antibody NB100-55327 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SMCX-Antibody-Western-Blot-NB100-55327-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SMCX Antibody [NB100-55327] - Detection of Human and Mouse Jarid1C by Western Blot. Samples: Whole cell lysate (50, 15 ug) from HeLa and (50 ug) from 293T, Jurkat, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Jarid1C antibody NB100-55327 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Chromatin Immunoprecipitation Sequencing: SMCX Antibody [NB100-55327] - Localization of Jarid1C Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-Jarid1C antibody NB100-55327 and analyzed by DNA sequencing. The figure illustrates the peak distribution of Jarid1C binding within a 500 Kb region of chromosome 1 as detected using anti-Jarid1C antibody NB100-55327. ChIP-seq validation performed by Diogenode, Denville, NJ.](http://images.novusbio.com/fullsize/SMCX-Antibody-Chromatin-Immunoprecipitation-Sequencing-NB100-55327-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: SMCX Antibody [NB100-55327] - Detection of human Jarid1C (SMCX) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Jarid1C antibody NB100-55327 used for IP at 6 ug per reaction. Jarid1C was also immunoprecipitated by rabbit anti-Jarid1C antibody NB100-55328. For blotting immunoprecipitated Jarid1C, NB100-55327 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SMCX-Antibody-Immunoprecipitation-NB100-55327-img0005.jpg)
![Western Blot: SMCX Antibody [NB100-55328] - Detection of Human JARID1C on HeLa whole cell lysate using NB100-55328. JARID1C was also immunoprecipitated by rabbit anti-JARID1C antibody NB100-55327.](http://images.novusbio.com/fullsize/SMCX-Antibody-Western-Blot-NB100-55328-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SMCX Antibody [NB100-55328] - Detection of Human JARID1C on HeLa whole cell lysate using NB100-55328. JARID1C was also immunoprecipitated by rabbit anti-JARID1C antibody NB100-55327.")
![Western Blot: SMCX Antibody [NBP2-55541] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/SMCX-Antibody-Western-Blot-NBP2-55541-img0002.jpg "Western Blot: SMCX Antibody [NBP2-55541] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: SMCX Antibody [NBP2-55541] - Staining of human cell line HEK 293 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/SMCX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55541-img0001.jpg)
![Western Blot: SMCX Antibody [NBP3-18659] - Figure 2. Western blot analysis using the antibody directed against SMCX Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against SMCX and with the preimmune serum (lane 1) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 175 kDa); the marker (in kDa) is shown on the left. Figure 2 shows that the additional bands at approximately 55 and 80 kDa are also present in the preimmune serum.](http://images.novusbio.com/fullsize/SMCX-Antibody-Western-Blot-NBP3-18659-img0001.jpg "Western Blot: SMCX Antibody [NBP3-18659] - Figure 2. Western blot analysis using the antibody directed against SMCX Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against SMCX and with the preimmune serum (lane 1) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 175 kDa); the marker (in kDa) is shown on the left. Figure 2 shows that the additional bands at approximately 55 and 80 kDa are also present in the preimmune serum.")
![ELISA: SMCX Antibody [NBP3-18659] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against SMCX . The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:7,000.](http://images.novusbio.com/fullsize/SMCX Antibody-ELISA-NBP3-18659-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SMCX Antibody [NBP2-55009] - Staining of human cell line HEK 293 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMCX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55009-img0001.jpg "Immunocytochemistry/Immunofluorescence: SMCX Antibody [NBP2-55009] - Staining of human cell line HEK 293 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Western Blot: SMCX Antibody [NBP2-20422] - Sample (30 ug of whole cell lysate) A: IMR32 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/SMCX-Antibody-Western-Blot-NBP2-20422-img0001.jpg "Western Blot: SMCX Antibody [NBP2-20422] - Sample (30 ug of whole cell lysate) A: IMR32 5% SDS PAGE gel, diluted at 1:10000.")
