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![Western Blot: HIRA Antibody [NBP3-04894] - Analysis of extracts from normal (control) and HIRA Rabbit pAb knockout (KO) 293T cells, using HIRA Rabbit pAb antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/HIRA-Antibody-Western-Blot-NBP3-04894-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HIRA Antibody [NBP3-04894] - Analysis of extracts from normal (control) and HIRA Rabbit pAb knockout (KO) 293T cells, using HIRA Rabbit pAb antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: HIRA Antibody [NBP3-04894] - Paraffin-embedded human colon carcinoma using HIRA antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HIRA-Antibody-Immunohistochemistry-Paraffin-NBP3-04894-img0003.jpg)
![Western Blot: HIRA Antibody [NBP3-04894] - Analysis of extracts of various cell lines, using HIRA antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/HIRA-Antibody-Western-Blot-NBP3-04894-img0004.jpg)
![Immunohistochemistry-Paraffin: HIRA Antibody [NBP3-04894] - Paraffin-embedded human gastric cancer using HIRA antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HIRA-Antibody-Immunohistochemistry-Paraffin-NBP3-04894-img0001.jpg)
![Immunohistochemistry-Paraffin: HIRA Antibody [NBP3-04894] - Paraffin-embedded human lung cancer using HIRA antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HIRA-Antibody-Immunohistochemistry-Paraffin-NBP3-04894-img0002.jpg)
![Immunohistochemistry-Paraffin: HIRA Antibody [NBP2-38636] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/HIRA-Antibody-Immunohistochemistry-Paraffin-NBP2-38636-img0006.jpg "Immunohistochemistry-Paraffin: HIRA Antibody [NBP2-38636] - Staining of human thyroid gland shows high expression.")
![Immunohistochemistry-Paraffin: HIRA Antibody [NBP2-38636] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/HIRA-Antibody-Immunohistochemistry-Paraffin-NBP2-38636-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HIRA Antibody [NBP2-38636] - Staining in human thyroid gland and liver tissues using anti-HIRA antibody. Corresponding HIRA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HIRA-Antibody-Immunohistochemistry-Paraffin-NBP2-38636-img0007.jpg)
![Western Blot: HIRA Antibody (SR1066) [NBP3-22291] - Western blot analysis using NBP3-22291 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22291_rabbit-hira-mab-sr1066-57202317215151.jpg "Western Blot: HIRA Antibody (SR1066) [NBP3-22291] - Western blot analysis using NBP3-22291 in HeLa cell lysate.")
![Western Blot: HIRA Antibody [NBP3-18665] - Figure 2. Western blot analysis using the antibody directed against HIRA (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse HIRA , diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (112 kDa) is indicated on the right.](http://images.novusbio.com/fullsize/HIRA-Antibody-Western-Blot-NBP3-18665-img0001.jpg "Western Blot: HIRA Antibody [NBP3-18665] - Figure 2. Western blot analysis using the antibody directed against HIRA (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse HIRA , diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (112 kDa) is indicated on the right.")
![ELISA: HIRA Antibody [NBP3-18665] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse HIRA . The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,300.](http://images.novusbio.com/fullsize/HIRA Antibody-ELISA-NBP3-18665-img0002.jpg)
![SDS-Page: Recombinant Human HIRA Protein [H00007290-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00007290-Q01-1.jpg "SDS-Page: Recombinant Human HIRA Protein [H00007290-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: HIRA Antibody [NBP3-04893] - Analysis of extracts of various cell lines, using HIRA antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic](http://images.novusbio.com/fullsize/HIRA-Antibody-Western-Blot-NBP3-04893-img0001.jpg "Western Blot: HIRA Antibody [NBP3-04893] - Analysis of extracts of various cell lines, using HIRA antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic")
![Immunocytochemistry/Immunofluorescence: HIRA Antibody [NBP2-55647] - Staining of human cell line HeLa shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/HIRA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55647-img0001.jpg "Immunocytochemistry/Immunofluorescence: HIRA Antibody [NBP2-55647] - Staining of human cell line HeLa shows localization to nucleoplasm.")
![Western Blot: HIRA Antibody [NBP2-87569] - Host: Rabbit. Target Name: HIRA. Sample Tissue: Human ACHN Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/HIRA-Antibody-Western-Blot-NBP2-87569-img0001.jpg "Western Blot: HIRA Antibody [NBP2-87569] - Host: Rabbit. Target Name: HIRA. Sample Tissue: Human ACHN Whole Cell lysates. Antibody Dilution: 1ug/ml")
![Western Blot: HIRA Antibody [NBP2-87568] - WB Suggested Anti-Hira Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Brain](http://images.novusbio.com/fullsize/HIRA-Antibody-Western-Blot-NBP2-87568-img0001.jpg "Western Blot: HIRA Antibody [NBP2-87568] - WB Suggested Anti-Hira Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Brain")
![Western Blot: HIRA Antibody [NBP2-87391] - Host: Rabbit. Target Name: HIRA. Sample Tissue: Human Neurofibroma Tumor lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/HIRA-Antibody-Western-Blot-NBP2-87391-img0001.jpg "Western Blot: HIRA Antibody [NBP2-87391] - Host: Rabbit. Target Name: HIRA. Sample Tissue: Human Neurofibroma Tumor lysates. Antibody Dilution: 1ug/ml")
![Western Blot: HIRA Antibody (6Q7Y6) [NBP3-16716] - Western blot analysis of extracts of various cell lines, using HIRA antibody (NBP3-16716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/HIRA-Antibody-6Q7Y6-Western-Blot-NBP3-16716-img0001.jpg "Western Blot: HIRA Antibody (6Q7Y6) [NBP3-16716] - Western blot analysis of extracts of various cell lines, using HIRA antibody (NBP3-16716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")