Hu,
Mu,
Ze,
Rt,
Po,
Ca,
Eq,
Pm,
RM,
SqSpecies Glossary
Reactivity: Hu, Mu, Ze, Rt, Po, Ca, Eq, Pm, RM, Sq
WB, ChIP, Flow, ICC/IF, IHC, IP, ChIP, KD
Applications: WB, ChIP, Flow, ICC/IF, IHC, IP, ChIP, KD
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-DDX21 Antibody NB100-1718 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1718-img0015.jpg "Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-DDX21 Antibody NB100-1718 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Flow Cytometry: DDX21 Antibody [NB100-1718] - An intracellular stain was performed on Jurkat Cells with DDX21Antibody NB100-1718 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).](http://images.novusbio.com/fullsize/DDX21-Antibody-Flow-Cytometry-NB100-1718-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with DDX21 Antibody conjugated to Alexa Fluor 488 (NB100-1718AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1718-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - ICC/IF detection of DDX21 in HeLa cells which were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-DDX21 [NB100-1718] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DylightTM 488 (green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DylightTM 550 (red) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue) [NBP2-31156].](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1718-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to Alexa Fluor 647 (NB100-1718AF647) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1718-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-DDX21 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1718-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NB100-1718] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-DDX21 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1718-img0014.jpg)
![Western Blot: DDX21 Antibody [NB100-1718] - Detection of Human DDX21 on HeLa whole cell lystate using NB100-1718. DDX21 was also immunoprecipitated using rabbit anti-DDX21 antibodies NB100-1716 and NB100-1717.](http://images.novusbio.com/fullsize/DDX21-Antibody-Western-Blot-NB100-1718-img0004.jpg)
![Immunohistochemistry: DDX21 Antibody [NB100-1718] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX21 used at a dilution of 1:200 (1ug/ml) . Detection: DAB.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-NB100-1718-img0007.jpg)
![Flow Cytometry: DDX21 Antibody - BSA Free [NB100-1718] - An intracellular stain was performed on A431 cells with DDX21 NB100-1718AF647 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/DDX21-Antibody---BSA-Free-Flow-Cytometry-NB100-1718-img0019.jpg)
![Immunohistochemistry-Paraffin: DDX21 Antibody [NB100-1718] - FFPE section of human larynx squamous cell carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-Paraffin-NB100-1718-img0005.jpg)
![Immunohistochemistry-Paraffin: DDX21 Antibody [NB100-1718] - FFPE section of mouse squamous cell carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-Paraffin-NB100-1718-img0006.jpg)
![Immunohistochemistry: DDX21 Antibody [NB100-1718] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX21 used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-NB100-1718-img0008.jpg)
![Flow Cytometry: DDX21 Antibody [NB100-1718] - An intracellular stain was performed on HeLa cells with DDX21 Antibody NB100-1718AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/DDX21-Antibody-Flow-Cytometry-NB100-1718-img0018.jpg)
![Immunohistochemistry-Paraffin: DDX21 Antibody [NBP1-83310] - Analysis in human appendix and skeletal muscle tissues using NBP1-83310 antibody. Corresponding DDX21 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-Paraffin-NBP1-83310-img0016.jpg "Immunohistochemistry-Paraffin: DDX21 Antibody [NBP1-83310] - Analysis in human appendix and skeletal muscle tissues using NBP1-83310 antibody. Corresponding DDX21 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: DDX21 Antibody [NBP1-83310] - Analysis in human cell lines Caco-2 and HeLa using Anti-DDX21 antibody. Corresponding DDX21 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/DDX21-Antibody-Western-Blot-NBP1-83310-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NBP1-83310] - Staining of human cell line U-251 MG shows localization to nucleus & nucleoli. Antibody staining shown in green.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83310-img0007.jpg)
![Immunohistochemistry-Paraffin: DDX21 Antibody [NBP1-83310] - Staining of human appendix shows strong positivity in nucleoli in lymphoid tissue.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-Paraffin-NBP1-83310-img0017.jpg)
![Immunohistochemistry-Paraffin: DDX21 Antibody [NBP1-83310] - Staining of human skeletal muscle shows no positivity in nucleoli in myocytes as expected.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-Paraffin-NBP1-83310-img0018.jpg)
![Western Blot: DDX21 Antibody [NBP2-38311] - Analysis using Anti-DDX21 antibody NBP2-38311 (A) shows similar pattern to independent antibody NBP1-83310 (B).](http://images.novusbio.com/fullsize/DDX21-Antibody-Western-Blot-NBP2-38311-img0011.jpg "Western Blot: DDX21 Antibody [NBP2-38311] - Analysis using Anti-DDX21 antibody NBP2-38311 (A) shows similar pattern to independent antibody NBP1-83310 (B).")
![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NBP2-38311] - Staining of human cell line U-2 OS shows localization to nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38311-img0004.jpg)
![Immunohistochemistry-Paraffin: DDX21 Antibody [NBP2-38311] - Staining of human appendix shows strong positivity in nucleoli in lymphoid tissue.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-Paraffin-NBP2-38311-img0013.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: DDX21 Antibody [NBP2-38311] - Western blot analysis in human cell lines Caco-2 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/DDX21-Antibody-Western-Blot-NBP2-38311-img0010.jpg)
![Immunohistochemistry-Paraffin: DDX21 Antibody [NBP2-38311] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-Paraffin-NBP2-38311-img0005.jpg)
![Immunohistochemistry-Paraffin: DDX21 Antibody [NBP2-38311] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-Paraffin-NBP2-38311-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DDX21 Antibody [NBP2-38311] - Staining in human appendix and skeletal muscle tissues using anti-DDX21 antibody. Corresponding DDX21 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-Paraffin-NBP2-38311-img0007.jpg)
![Immunohistochemistry-Paraffin: DDX21 Antibody [NBP2-38311] - Analysis in human appendix and skeletal muscle tissues using NBP2-38311 antibody. Corresponding DDX21 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-Paraffin-NBP2-38311-img0012.jpg)
![Immunohistochemistry-Paraffin: DDX21 Antibody [NBP2-38311] - Staining of human skeletal muscle shows no positivity in nucleoli in myocytes as expected.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunohistochemistry-Paraffin-NBP2-38311-img0014.jpg)
![Western Blot: DDX21 Antibody [NBP2-97537] - Anti-DDX21 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 87 kDa. Observed band size: 95 kDa](http://images.novusbio.com/fullsize/DDX21-Antibody-Western-Blot-NBP2-97537-img0004.jpg "Western Blot: DDX21 Antibody [NBP2-97537] - Anti-DDX21 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 87 kDa. Observed band size: 95 kDa")
![Immunocytochemistry/Immunofluorescence: DDX21 Antibody [NBP2-97537] - Immunofluorescence staining of DDX21 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human DDX21 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97537-img0001.jpg)
![Immunoprecipitation: DDX21 Antibody [NBP2-97537] - DDX21 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 4 ul anti-DDX21 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-DDX21 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 87 kDa. Observed band size: 95 kDa](http://images.novusbio.com/fullsize/DDX21-Antibody-Immunoprecipitation-NBP2-97537-img0005.jpg)
![Western Blot: DDX21 Antibody [NB100-1717] - Detection of Human DDX21 on HeLa whole cell lysate using NB100-1717. DDX21 was also immunoprecipitated using rabbit anti-DDX21 antibodies NB100-1716 and NB100-1718.](http://images.novusbio.com/fullsize/DDX21-Antibody-Western-Blot-NB100-1717-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DDX21 Antibody [NB100-1717] - Detection of Human DDX21 on HeLa whole cell lysate using NB100-1717. DDX21 was also immunoprecipitated using rabbit anti-DDX21 antibodies NB100-1716 and NB100-1718.")
![Western Blot: DDX21 Antibody [NB100-1716] - Detection of Human DDX21 on HeLa whole cell lysate using NB100-1716. DDX21 was also immunoprecipitated using rabbit anti-DDX21 antibodies NB100-1717 and NB100-1718.](http://images.novusbio.com/fullsize/DDX21-Antibody-Western-Blot-NB100-1716-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DDX21 Antibody [NB100-1716] - Detection of Human DDX21 on HeLa whole cell lysate using NB100-1716. DDX21 was also immunoprecipitated using rabbit anti-DDX21 antibodies NB100-1717 and NB100-1718.")
![Western Blot: DDX21 Overexpression Lysate (Adult Normal) [NBL1-09787] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDX21.](http://images.novusbio.com/fullsize/DDX21-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09787-img0002.jpg "Western Blot: DDX21 Overexpression Lysate (Adult Normal) [NBL1-09787] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDX21.")
![Western Blot: DDX21 Antibody (S04-4J2) [NBP3-19619] - Western blot detection of DDX21 in Hela cell lysates using NBP3-19619 (1:1000 diluted). Predicted band size: 87kDa. Observed band size: 87kDa.](http://images.novusbio.com/fullsize/DDX21-Antibody-S04-4J2-Western-Blot-NBP3-19619-img0001.jpg "Western Blot: DDX21 Antibody (S04-4J2) [NBP3-19619] - Western blot detection of DDX21 in Hela cell lysates using NBP3-19619 (1:1000 diluted). Predicted band size: 87kDa. Observed band size: 87kDa.")