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![Western Blot: CYLN2 Antibody [NBP1-85569] - Analysis in human cell lines SK-MEL-30 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/CYLN2-Antibody-Western-Blot-NBP1-85569-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: CYLN2 Antibody [NBP1-85569] - Analysis in human cell lines SK-MEL-30 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CYLN2 Antibody [NBP1-85569] - Staining in human cerebral cortex and kidney tissues using anti-CLIP2 antibody. Corresponding CLIP2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CYLN2-Antibody-Immunohistochemistry-Paraffin-NBP1-85569-img0014.jpg)
![Immunohistochemistry-Paraffin: CYLN2 Antibody [NBP1-85569] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/CYLN2-Antibody-Immunohistochemistry-Paraffin-NBP1-85569-img0008.jpg)
![Immunohistochemistry-Paraffin: CYLN2 Antibody [NBP1-85569] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/CYLN2-Antibody-Immunohistochemistry-Paraffin-NBP1-85569-img0012.jpg)
![Immunohistochemistry-Paraffin: CYLN2 Antibody [NBP1-85569] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/CYLN2-Antibody-Immunohistochemistry-Paraffin-NBP1-85569-img0013.jpg)
![Western Blot: CYLN2 Antibody [H00007461-B01P-50ug] - CLIP2 MaxPab polyclonal antibody. Western Blot analysis of CLIP2 expression in rat brain.](http://images.novusbio.com/fullsize/CYLN2-Antibody-Western-Blot-H00007461-B01P-50ug-img0002.jpg "Western Blot: CYLN2 Antibody [H00007461-B01P-50ug] - CLIP2 MaxPab polyclonal antibody. Western Blot analysis of CLIP2 expression in rat brain.")
![Western Blot: CYLN2 Antibody [H00007461-B01P-50ug] - Analysis of CLIP2 expression in transfected 293T cell line (H00007461-T01) by CLIP2 MaxPab polyclonal antibody. Lane 1: CLIP2 transfected lysate(115.06 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CYLN2-Antibody-Western-Blot-H00007461-B01P-50ug-img0001.jpg)
![Western Blot: CYLN2 Antibody [NBP1-78744] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-CLIP115 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CLIP115 was also immunoprecipitated by rabbit anti-CLIP115 antibody which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/CYLN2-Antibody-Western-Blot-NBP1-78744-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CYLN2 Antibody [NBP1-78744] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-CLIP115 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CLIP115 was also immunoprecipitated by rabbit anti-CLIP115 antibody which recognizes an upstream epitope.")
![Western Blot: CYLN2 Antibody (3H5) [H00007461-M07] - Analysis of CLIP2 expression in transfected 293T cell line by CLIP2 monoclonal antibody (M07), clone 3H5. Lane 1: CLIP2 transfected lysate (Predicted MW: 115.06 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CYLN2-Antibody-3H5-Western-Blot-H00007461-M07-img0001.jpg "Western Blot: CYLN2 Antibody (3H5) [H00007461-M07] - Analysis of CLIP2 expression in transfected 293T cell line by CLIP2 monoclonal antibody (M07), clone 3H5. Lane 1: CLIP2 transfected lysate (Predicted MW: 115.06 KDa). Lane 2: Non-transfected lysate.")
![Immunoprecipitation: CYLN2 Antibody [NBP1-78743] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CLIP115 antibody used for IP at 6 mcg/mg lysate. CLIP115 was also immunoprecipitated by rabbit anti-CLIP115 antibody NBP1-78744 which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/CYLN2-Antibody-Immunoprecipitation-NBP1-78743-img0003.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: CYLN2 Antibody [NBP1-78743] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CLIP115 antibody used for IP at 6 mcg/mg lysate. CLIP115 was also immunoprecipitated by rabbit anti-CLIP115 antibody NBP1-78744 which recognizes a downstream epitope.")