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![Western Blot: Use1/UBE2Z Antibody [NBP1-82785] - Analysis in human cell line RPMI-8226.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Western-Blot-NBP1-82785-img0007.jpg "Western Blot: Use1/UBE2Z Antibody [NBP1-82785] - Analysis in human cell line RPMI-8226.")
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP1-82785-img0010.jpg)
![Western Blot: Use1/UBE2Z Antibody [NBP1-82785] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Western-Blot-NBP1-82785-img0005.jpg)
![Western Blot: Use1/UBE2Z Antibody [NBP1-82785] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Western-Blot-NBP1-82785-img0006.jpg)
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP1-82785-img0002.jpg)
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP1-82785-img0008.jpg)
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human lymphoid node shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP1-82785-img0009.jpg)
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP1-82785-img0011.jpg)
![Western Blot: Use1/UBE2Z Antibody [NBP2-97553] - Anti-Use1/UBE2Z rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Western-Blot-NBP2-97553-img0003.jpg "Western Blot: Use1/UBE2Z Antibody [NBP2-97553] - Anti-Use1/UBE2Z rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa")
![Immunocytochemistry/Immunofluorescence: Use1/UBE2Z Antibody [NBP2-97553] - Immunofluorescence staining of Use1/UBE2Z in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Use1/UBE2Z polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97553-img0002.jpg)
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP2-97553] - Immunochemical staining of human Use1/UBE2Z in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP2-97553-img0004.jpg)
![Immunoprecipitation: Use1/UBE2Z Antibody [NBP2-97553] - Use1/UBE2Z was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-Use1/UBE2Z rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Use1/UBE2Z rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunoprecipitation-NBP2-97553-img0001.jpg)
![Western Blot: Use1/UBE2Z Antibody [NBP2-38568] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Western-Blot-NBP2-38568-img0002.jpg "Western Blot: Use1/UBE2Z Antibody [NBP2-38568] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunohistochemistry: Use1/UBE2Z Antibody [NBP2-38568] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-NBP2-38568-img0001.jpg)
![Western Blot: Use1 Antibody [NBP2-20822] - Sample (30 ug of whole cell lysate) A: NT2D1 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Use1-Antibody-Western-Blot-NBP2-20822-img0001.jpg "Western Blot: Use1 Antibody [NBP2-20822] - Sample (30 ug of whole cell lysate) A: NT2D1 15% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Use1 Antibody [NBP2-20822] - Immunofluorescence analysis of methanol-fixed MCF-7, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Use1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20822-img0002.jpg)
![Western Blot: Use1/UBE2Z Antibody [NBP1-91539] - SP2/0 cell lysate, concentration 2.5 ug/ml.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Western-Blot-NBP1-91539-img0001.jpg "Western Blot: Use1/UBE2Z Antibody [NBP1-91539] - SP2/0 cell lysate, concentration 2.5 ug/ml.")
![Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-91539] - Mouse Skin tissue, 4-8ug/ml.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Immunohistochemistry-Paraffin-NBP1-91539-img0002.jpg)
![Western Blot: Use1/UBE2Z Antibody [NBP2-95110] - Analysis of extracts of various cell lines, using Use1/UBE2Z at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/Use1-UBE2Z-Antibody-Western-Blot-NBP2-95110-img0003.jpg "Western Blot: Use1/UBE2Z Antibody [NBP2-95110] - Analysis of extracts of various cell lines, using Use1/UBE2Z at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.")
![Western Blot: Use1 Overexpression Lysate (Adult Normal) [NBL1-17636] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Use1.](http://images.novusbio.com/fullsize/Use1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17636-img0002.jpg "Western Blot: Use1 Overexpression Lysate (Adult Normal) [NBL1-17636] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Use1.")