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![Western Blot: PHF8 Antibody [NB100-93313] - Detection of human and mouse PHF8 by western blot. Samples: Whole cell lysate (15 and 50 ug) from Jurkat, HeLa, HEK293T, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF8 antibody NB100-93313 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PHF8-Antibody-Western-Blot-NB100-93313-img0009.jpg "Western Blot: PHF8 Antibody [NB100-93313] - Detection of human and mouse PHF8 by western blot. Samples: Whole cell lysate (15 and 50 ug) from Jurkat, HeLa, HEK293T, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF8 antibody NB100-93313 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93313] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- PHF8 used at a dilution of 1:200 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PHF8-Antibody-Immunohistochemistry-Paraffin-NB100-93313-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PHF8 Antibody [NB100-93313] - Detection of Human PHF8 on HeLa whole cell lysate using NB100-93313. PHF8 was also immunoprecipitated by rabbit anti-PHF8 antibody NB100-93314.](http://images.novusbio.com/fullsize/PHF8-Antibody-Western-Blot-NB100-93313-img0003.jpg)
![Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93313] - Section of human small cell lung cancer. Antibody: Affinity purified rabbit anti- PHF8 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PHF8-Antibody-Immunohistochemistry-Paraffin-NB100-93313-img0010.jpg)
![Western Blot: PHF8 Antibody [NB100-93314] - Detection of Human PHF8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, HeLa (RIPA), 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PHF8 antibody NB100-93314 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PHF8-Antibody-Western-Blot-NB100-93314-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: PHF8 Antibody [NB100-93314] - Detection of Human PHF8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, HeLa (RIPA), 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PHF8 antibody NB100-93314 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Knockdown Validated: PHF8 Antibody [NB100-93314] - Western blot analysis showing knockdown of PHF8 by shRNA against PHF8. LNCaP cells were infected with a lentiviral shPHF8 and western blot analysis was carried out with anti-PHF8 antibody. Image collected and cropped by CiteAb from the llowing publication (nature.com/articles/oncsis201674), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PHF8-Antibody-Western-Blot-NB100-93314-img0009.jpg)
![Western Blot: PHF8 Antibody [NB100-93314] - PHF8 interacts with the AR & functions as an AR coactivator. (a) PHF8 interacts with AR independent of DHT. HEK293T cells were transiently transfected with plasmids expressing Flag-tagged PHF8 & GFP-tagged AR. Cells were treated with or without 10 nM DHT for 48 h & the cell lysates were used for immunoprecipitation with antibodies against either Flag or GFP. Western blotting was conducted with antibodies as indicated. (b) PHF8 interacts with AR independent of its demethylase activity. HEK293T cells were transiently transfected with plasmids expressing Flag-tagged PHF8 (H247A) mutant & GFP-tagged AR. The interaction assay by co-immunoprecipitation was performed as described above. (c) In LNCaP cells endogenous PHF8 interacts with AR. LNCaP cells were treated with 10 nM DHT for 48 h & the cell lysates were used for immunoprecipitation with antibodies against either PHF8 or AR. Western blotting was conducted with AR or PHF8 antibody as indicated. (d) PHF8 enhances AR transcriptional activation in a demethylase activity-dependent manner. HeLa cells were co-transfected with the 4 × UAS-TK-luc reporter, the plasmids expressing the fusion protein Gal-AR, & increased amount of either the wild-type PHF8 or mutant PHF8 (H247A) as indicated. Cells were cultured with or without 10 nM R1881 overnight. The relative luciferase activities in the cell lysates were normalized to renilla luciferase activity. The relative luciferase activities are presented as mean±s.e.m. of three independent transfections & the levels of PHF8 were estimated by western blot as depicted in the bottom panel. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/oncsis201674), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-93314_rabbit-polyclonal-phf8-antibody-31020241628491.jpg)
![Western Blot: PHF8 Antibody [NBP1-83079] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/PHF8-Antibody-Western-Blot-NBP1-83079-img0005.jpg "Western Blot: PHF8 Antibody [NBP1-83079] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: PHF8 Antibody [NBP1-83079] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/PHF8-Antibody-Immunohistochemistry-Paraffin-NBP1-83079-img0006.jpg)
![Western Blot: PHF8 Antibody (2H9D4) [NBP3-15910] - Western blot analysis of extracts of HeLa cells, using PHF8 antibody (NBP3-15910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/PHF8-Antibody-2H9D4-Western-Blot-NBP3-15910-img0001.jpg "Western Blot: PHF8 Antibody (2H9D4) [NBP3-15910] - Western blot analysis of extracts of HeLa cells, using PHF8 antibody (NBP3-15910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: PHF8 Antibody (2H9D4) [NBP3-15910] - Western blot analysis of extracts of various cell lines, using PHF8 antibody (NBP3-15910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/PHF8-Antibody-2H9D4-Western-Blot-NBP3-15910-img0002.jpg)
![Western Blot: PHF8 Antibody (SR1157) [NBP3-21826] - Western blot analysis using NBP3-21826 in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21826_rabbit-phf8-mab-sr1157-57202316112127.jpg "Western Blot: PHF8 Antibody (SR1157) [NBP3-21826] - Western blot analysis using NBP3-21826 in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.")
![Western Blot: PHF8 Antibody [NBP2-62797] - E14TG2A mouse embryonic stem cells were transfected with a conditional allele of PHF8. Nuclear extracts from wild type cells (WT) and from 2 clones (F1, B8) with an active and a targeted allele (F1ko, B8ko), respectively, were analysed using the antibody against PHF8.](http://images.novusbio.com/fullsize/PHF8-Antibody-Western-Blot-NBP2-62797-img0002.jpg "Western Blot: PHF8 Antibody [NBP2-62797] - E14TG2A mouse embryonic stem cells were transfected with a conditional allele of PHF8. Nuclear extracts from wild type cells (WT) and from 2 clones (F1, B8) with an active and a targeted allele (F1ko, B8ko), respectively, were analysed using the antibody against PHF8.")
![ELISA: PHF8 Antibody [NBP2-62797] - To determine the titer of the antibody, an analysis was performed using a serial dilution of the antibody directed against human PHF8, crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:6,500.](http://images.novusbio.com/fullsize/PHF8-Antibody-ELISA-NBP2-62797-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PHF8 Antibody [NBP2-56147] - Staining of human cell line SiHa shows localization to nucleus.](http://images.novusbio.com/fullsize/PHF8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56147-img0001.jpg "Immunocytochemistry/Immunofluorescence: PHF8 Antibody [NBP2-56147] - Staining of human cell line SiHa shows localization to nucleus.")