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Products Related to Gene-ID: 2294

15 Results for "2294"

AF4798

FoxF1 Antibody [Unconjugated]

(29 Publications)

100 μg

AF4798

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FoxF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FoxF1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FoxF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FoxF1 at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Loss of FOXF1 promotes fundamental cellular processes in LR-MSCs. (A) mRNA was isolated from fibrotic and non-fibrotic LR-MSCs derived from bronchoalveolar lavage fluid of transplant patients, and FOXF1 expression was analyzed by real-time PCR. Values: Means ± SEM; n = 9 (non-Fib-MSCs); n = 8 (Fib-MSCs); **p < 0.0034. (B) Protein lysates from fibrotic and non-fibrotic LR-MSCs were analyzed for FOXF1 and GAPDH by western blotting. Graph shows densitometry analyses of these immunoblots. Values: Means ± SEM; n = 16; **p < 0.0086. (C) LR-MSCs were transfected with scrambled or FOXF1-specific siRNA and confirmed by real-time PCR. Values: Means ± SEM; n = 7; ***p < 0.0005. (D) Protein lysates from (A) were subjected to immunoblotting with antibodies against FOXF1 and GAPDH. (E–I) Gene regulation due to FOXF1 silencing was analyzed by Affymetrix gene array in 3 lines of LR-MSCs. Data reflects fold changes ≥ 1.5, and an adjusted p < 0.01. (E) Diagram showing the number of upregulated and downregulated genes. (F) Gene–gene interaction network (using STRING database) showing associations due to FOXF1-silencing. (G–I) Heatmaps showing two-fold Log changes are presented for positive regulation of cell cycle ((G) GO:0045787), inflammatory response ((H) GO:0006954), and regulation of cell migration ((I) GO:0030334). Note: Full length blots for Fig. 1B and Fig. 1D are provided in Supplementary Fig. S1 and S2. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33277571), licensed under a CC-BY license. Not internally tested by R&D Systems.

Loss of FOXF1 promotes fundamental cellular processes in LR-MSCs. (A) mRNA was isolated from fibrotic and non-fibrotic LR-MSCs derived from bronchoalveolar lavage fluid of transplant patients, and FOXF1 expression was analyzed by real-time PCR. Values: Means ± SEM; n = 9 (non-Fib-MSCs); n = 8 (Fib-MSCs); **p < 0.0034. (B) Protein lysates from fibrotic and non-fibrotic LR-MSCs were analyzed for FOXF1 and GAPDH by western blotting. Graph shows densitometry analyses of these immunoblots. Values: Means ± SEM; n = 16; **p < 0.0086. (C) LR-MSCs were transfected with scrambled or FOXF1-specific siRNA and confirmed by real-time PCR. Values: Means ± SEM; n = 7; ***p < 0.0005. (D) Protein lysates from (A) were subjected to immunoblotting with antibodies against FOXF1 and GAPDH. (E–I) Gene regulation due to FOXF1 silencing was analyzed by Affymetrix gene array in 3 lines of LR-MSCs. Data reflects fold changes ≥ 1.5, and an adjusted p < 0.01. (E) Diagram showing the number of upregulated and downregulated genes. (F) Gene–gene interaction network (using STRING database) showing associations due to FOXF1-silencing. (G–I) Heatmaps showing two-fold Log changes are presented for positive regulation of cell cycle ((G) GO:0045787), inflammatory response ((H) GO:0006954), and regulation of cell migration ((I) GO:0030334). Note: Full length blots for Fig. 1B and Fig. 1D are provided in Supplementary Fig. S1 and S2. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33277571), licensed under a CC-BY license. Not internally tested by R&D Systems.

ATX-dependent cell migration in FOXF1-silenced LR-MSCs. (A) LR-MSCs were transfected with scrambled or FOXF1-specific siRNA. 24 h later, these LR-MSCs were transfected with scrambled or ATX-specific siRNA. Immunoblotting was performed to confirm RNAi-mediated FOXF1 and ATX silencing efficacy. n = 5 per group. (B) Migration assay was conducted in LR-MSCs transfected with scrambled or siRNA specific to FOXF1, ATX, or both FOXF1- and ATX-specific siRNA. Representative images of cell migration are shown. (C) Quantification of (B), n = 5, ***p < 0.0003. (D) LR-MSCs transfected with scrambled or FOXF1-specific siRNA were treated with the ATX inhibitor, PF-8380 (1 μM) and migration assay was performed. Values: Means ± SEM. n = 5, **p < 0.0142, *p < 0.0325. (E,F) Migration assays are shown with LR-MSCs transfected with scrambled or FOXF1-specific siRNA, and then treated with the LPA1 inihibitor, VPC12249 (1 μM) (E), or subjected to lentivirus-mediated shRNA interference against LPAR1 (F). Values: Means ± SEM. n = 5. ***p < 0.0002, ****p < 0.0001. Note: Full length blots for Fig. 3A are provided in Supplementary Fig. S5. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33277571), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4798-SP

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NBP1-84670

FoxF1 Antibody

(1 Publication)

0.1 ml

NBP1-84670

Western Blot: FoxF1 Antibody [NBP1-84670] - Analysis in human lung tissue.

Immunocytochemistry/Immunofluorescence: FoxF1 Antibody [NBP1-84670] - Staining of human cell line BJ shows localization to nucleoplasm. Antibody staining is shown in green.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-87467

FoxF1 Antibody

100 ul

NBP2-87467

Western Blot: FoxF1 Antibody [NBP2-87467] - Host: Rabbit. Target Name: FOXF1. Sample Tissue: Human Hela. Antibody Dilution: 1.0ug/ml

Immunohistochemistry: FoxF1 Antibody [NBP2-87467] - Human kidney

Western Blot: FoxF1 Antibody [NBP2-87467] - Host: Rabbit. Target: FOXF1. Positive control (+): Hela (HL). Negative control (-): A549 (N03). Antibody concentration: 1ug/ml

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-87469

FoxF1 Antibody

100 ul

NBP2-87469

Western Blot: FoxF1 Antibody [NBP2-87469] - WB Suggested Anti-Foxf1a Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Mouse Heart

Immunohistochemistry: FoxF1 Antibody [NBP2-87469] - Sample Type: Mouse E 18.5 Gut. Dilution: 1:1000

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-22241

FoxF1 Antibody (SR1668)

100 ul

NBP3-22241

Western Blot: FoxF1 Antibody (SR1668) [NBP3-22241] - Western blot analysis using NBP3-22241 in HeLa cell lysate.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-16637

FoxF1 Antibody (8F6G3)

100 ul

NBP3-16637

Western Blot: FoxF1 Antibody (8F6G3) [NBP3-16637] - Analysis of extracts of various cell lines, using FoxF1 antibody (NBP3-16637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-87468

FoxF1 Antibody

100 ul

NBP2-87468

Western Blot: FoxF1 Antibody [NBP2-87468] - WB Suggested Anti-FOXF1A Antibody Titration: 2.5ug/ml. ELISA Titer: 1:12500. Positive Control: SP2/0 cell lysate

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-87466

FoxF1 Antibody

100 ul

NBP2-87466

Western Blot: FoxF1 Antibody [NBP2-87466] - WB Suggested Anti-FOXF1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBL1-10803

FoxF1 Overexpression Lysate

0.1 mg

NBL1-10803

Western Blot: FOXF1 Overexpression Lysate (Adult Normal) [NBL1-10803] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FOXF1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP3-04753

FoxF1 Antibody - Azide and BSA Free

100 ul

NBP3-04753

Western Blot: FoxF1 Antibody [NBP3-04753] - Analysis of extracts of mouse stomach, using FoxF1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-04753-20ul

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NBP2-98870

FoxF1 Antibody

100 ul

NBP2-98870

Immunohistochemistry-Paraffin: FoxF1 Antibody [NBP2-98870] - Immunochemical staining of human FoxF1 in human kidney with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-98870AF350
Alexa Fluor 405	NBP2-98870AF405
Alexa Fluor 488	NBP2-98870AF488
Alexa Fluor 532	NBP2-98870AF532
Alexa Fluor 594	NBP2-98870AF594
Alexa Fluor 647	NBP2-98870AF647
Alexa Fluor 700	NBP2-98870AF700
Alexa Fluor 750	NBP2-98870AF750
Allophycocyanin	NBP2-98870APC
Biotin	NBP2-98870B
CoraFluor 1	NBP2-98870CL1
DyLight 350	NBP2-98870UV
DyLight 405	NBP2-98870V
DyLight 488	NBP2-98870G
DyLight 550	NBP2-98870R
DyLight 594	NBP2-98870DL594
DyLight 650	NBP2-98870C
DyLight 680	NBP2-98870FR
DyLight 755	NBP2-98870IR
FITC	NBP2-98870F
HRP	NBP2-98870H
Janelia Fluor 525	NBP2-98870JF525
Janelia Fluor 549	NBP2-98870JF549
Janelia Fluor 585	NBP2-98870JF585
Janelia Fluor 635	NBP2-98870JF635
Janelia Fluor 646	NBP2-98870JF646
Janelia Fluor 669	NBP2-98870JF669
PE	NBP2-98870PE
PerCP	NBP2-98870PCP
mFluor Violet 450 SE	NBP2-98870MFV450
mFluor Violet 500 SE	NBP2-98870MFV500
mFluor Violet 610 SE	NBP2-98870MFV610

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H00002294-M05

FoxF1 Antibody (3D1)

0.1 mg

H00002294-M05

ELISA: FoxF1 Antibody (3D1) [H00002294-M05] - Detection limit for recombinant GST tagged FOXF1 is approximately 1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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