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![Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Gene and protein expression of components of the PAR-1/SphK/S1P axis. Western blot detection and quantification of the protein expression of PAR-1, S1PR1, SphK1, and SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for 6 h. The data are presented as the mean +/- SD (n = 3), *p < 0.05. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fnmol.2020.00114/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Western-Blot-NB120-11424-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Gene and protein expression of components of the PAR-1/SphK/S1P axis. Western blot detection and quantification of the protein expression of PAR-1, S1PR1, SphK1, and SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for 6 h. The data are presented as the mean +/- SD (n = 3), *p < 0.05. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fnmol.2020.00114/full) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NB120-11424] - Immunofluorescence of PAR-1 and S1PR1 in spinal cord tissues of mice induced by EAE. Mice subjected to EAE modeling were treated with vehicle (physiological saline), Dab, or PAR-1-inh. Tissues were stained for (B) S1PR1 in green and nuclei (DAPI) in blue. Scale bar = 100 um. Green fluorescence was quantified by measuring the relative mean IOD. The data are presented as the mean +/- SD (n = 6), *p < 0.05. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/articles/10.3389/fnmol.2020.00114/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11424-img0008.jpg)
![Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Analysis of S1P1 from transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Western-Blot-NB120-11424-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NB120-11424] - Immunofluorescence staining of S1P1 in transfected CCL39 cells using NB120-11424.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11424-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NB120-11424] - Immunofluorescence of proteins related to S1P1/EDG-1 signaling in astrocytes. Astrocytes were treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only) for 6 h. Cells were stained for S1P1/EDG-1 receptor 1 in green and nuclei (DAPI) in blue. Scale bar = 100 um. Green fluorescence was quantified by measuring the relative mean IOD. The data are presented as the mean +/- SD (n = 3), *p < 0.05. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fnmol.2020.00114/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11424-img0009.jpg)
![Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Gene & protein expression of components of the PAR-1/SphK/S1P axis. qRT-PCR analysis of the gene expression of (A) PAR-1, (B) S1PR1, (C) SphK1, & (D) SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for 0 h, 1 h, or 6 h. Western blot detection & quantification of the protein expression of PAR-1, S1PR1, SphK1, & SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for (E) 0 h, (F) 1 h, or (G) 6 h. The data are presented as the mean ± SD (n = 3), *p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32694981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-11424_rabbit-polyclonal-s1p1-edg-1-antibody-310202415175259.jpg)
![Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Expression of sphingosine-1-phosphate (S1P), interleukin (IL)-1 beta , & protease-activated receptor-1 (PAR-1) in isolated astrocytes. (A) Isolated & purified astrocytes were identified by bright-field microscopy & immunofluorescence for glial fibrillary acid protein (GFAP; red). Nuclei (DAPI) were labeled in blue. Scale bar = 100 μm. (B) Western blot & quantitative reverse transcription polymerase chain reaction (qRT-PCR) of the protein & mRNA expression, respectively, of S1P 0, 1, & 6 h after astrocytes were treated with lipopolysaccharide (LPS) or thrombin & Dabigatran (Dab) or PAR-1-inh (LPS only). (D) qRT-PCR & (E) enzyme-linked immunosorbent assay (ELISA) of the mRNA expression & secretion, respectively, of IL-1 beta 0, 1, & 6 h after astrocytes were treated with LPS or thrombin & Dab or PAR-1-inh (LPS only). (F) Immunofluorescence of astrocytes & quantification of relative mean integrated optical density (IOD; green fluorescence). PAR-1 was stained in green & nuclei (DAPI) were stained in blue. Scale bar = 100 μm. For (B–F), data are presented as the mean ± SD (n = 3). *p < 0.05; #p < 0.05 vs. the same group at 0 h; &p < 0.05 vs. the same group at 1 h. ns, not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32694981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-11424_rabbit-polyclonal-s1p1-edg-1-antibody-310202416205112.jpg)
![Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Western-Blot-NBP2-67129-img0011.jpg "Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0009.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse heart tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0008.jpg)
![Flow Cytometry: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of Jurkat cells with EDG1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Flow-Cytometry-NBP2-67129-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0003.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0004.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human kidney tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0005.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human liver tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0006.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse brain tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0007.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (MM0045-21L9) [NB110-93513] - Normal Human Placenta Tissue](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-MM0045-21L9-Immunohistochemistry-Paraffin-NB110-93513-img0003.jpg "Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (MM0045-21L9) [NB110-93513] - Normal Human Placenta Tissue")
![Flow Cytometry: S1P1/EDG-1 Antibody (MM0045-21L9) [NB110-93513] - Analysis using the Alexa Fluor (R) 488 conjugate of NB110-93513. Staining of S1P1 in white blood cells from human blood using anti-S1P1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-MM0045-21L9-Flow-Cytometry-NB110-93513-img0005.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody [NBP2-27291] - Analysis of S1P1/EDG-1 in FFPE human brain tissue using an isotype control (top left) and this antibody (bottom left, right)](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27291-img0002.jpg "Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody [NBP2-27291] - Analysis of S1P1/EDG-1 in FFPE human brain tissue using an isotype control (top left) and this antibody (bottom left, right)")
![Western Blot: S1P1/EDG-1 Antibody (9R8N3) [NBP3-16311] - Western blot analysis of extracts of various cell lines, using S1P1/EDG-1 Rabbit mAb (NBP3-16311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-9R8N3-Western-Blot-NBP3-16311-img0001.jpg "Western Blot: S1P1/EDG-1 Antibody (9R8N3) [NBP3-16311] - Western blot analysis of extracts of various cell lines, using S1P1/EDG-1 Rabbit mAb (NBP3-16311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![SDS-Page: Recombinant Human S1P1/EDG-1 Protein [H00001901-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001901-Q01-1.jpg "SDS-Page: Recombinant Human S1P1/EDG-1 Protein [H00001901-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NBP2-58024] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58024-img0001.jpg "Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NBP2-58024] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.")
![ELISA: Mouse S1P1/EDG-1 ELISA Kit (Colorimetric) [NBP2-82426] - Samples were spiked with high concentrations of Mouse S1P1/EDG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82426_mouse-s1p1-edg-1-elisa-kit-colorimetric-132202416121344.jpg "ELISA: Mouse S1P1/EDG-1 ELISA Kit (Colorimetric) [NBP2-82426] - Samples were spiked with high concentrations of Mouse S1P1/EDG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse S1P1/EDG-1 ELISA Kit (Colorimetric) [NBP2-82426] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82426_mouse-s1p1-edg-1-elisa-kit-colorimetric-4320249133224.jpg)
![Western Blot: S1P1/EDG-1 Antibody (4F2) [NBP3-15216] - Positive WB detected in: SH-SY5Y whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U251 whole cell lysate. All lanes: S1P1/EDG-1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43 KDa. Observed band size: 43 KDa.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-4F2-Western-Blot-NBP3-15216-img0001.jpg "Western Blot: S1P1/EDG-1 Antibody (4F2) [NBP3-15216] - Positive WB detected in: SH-SY5Y whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U251 whole cell lysate. All lanes: S1P1/EDG-1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43 KDa. Observed band size: 43 KDa.")