![Western Blot: MUC4 Antibody [NBP1-52193] - MUC4 is shown in the green channel, with beta-tubulin in red channel and Precision Plus dual color protein ladder in the left. The main band is about 60 kDa, with another band about 40 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/MUC-4-Antibody-Western-Blot-NBP1-52193-img0010.jpg "Western Blot: MUC4 Antibody [NBP1-52193] - MUC4 is shown in the green channel, with beta-tubulin in red channel and Precision Plus dual color protein ladder in the left. The main band is about 60 kDa, with another band about 40 kDa. Image from verified customer review.")
| Immunogen | A partial recombinant protein made to an internal region of the human MUC4 protein beta chain(within residues 5150-5300). [Swiss-Prot Q99102] |
| Localization | MUC4 beta chain: Cell membrane; Single-pass membrane protein. |
| Specificity | This antibody is specific for the beta chain of MUC4. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MUC4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||||
| Application Notes | In Western blot bands are seen ~78 kDa representing MUC4 beta and ~50 kDa. The identity of the 50 kDa band is unknown. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Does not work for IP as per customer feedback. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
||||
| Control |
|
||||
| Reviewed Applications |
|
||||
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Immunohistochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunohistochemistry_20892.jpg)
![Immunocytochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunocytochemistry__Immunofluorescence_21143.jpg)
![Western Blot EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Western_Blot_19925.jpg)
![Neutralization ErbB3/Her3 Antibody (66223) [Unconjugated]](https://images.novusbio.com/images2/ErbB3_MAB3481_Block_Neutralize_9158.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
WB | Mouse | 01/24/2017 |
Summary
|
Secondary Antibodies |
Isotype Controls |
Research Areas for MUC4 Antibody (NBP1-52193)Find related products by research area.
|
|
MUC4 (Mucin-4) Mucus is the viscous secretion that covers epithelial surfaces (trachea, colon, and cervix) and consists of twenty highly glycosylated proteins called mucins. The mucin family all are high-molecular weight proteins with oligosaccharides attached to th... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: MUC4 Antibody [NBP1-52193] - Staining of MUC4 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MUC-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-52193-img0004.jpg "Immunocytochemistry/Immunofluorescence: MUC4 Antibody [NBP1-52193] - Staining of MUC4 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: MUC4 Antibody [NBP1-52193] - Staining of MUC4 in mouse prostate](http://images.novusbio.com/fullsize/MUC-4-Antibody-Immunohistochemistry-NBP1-52193-img0005.jpg "Immunohistochemistry: MUC4 Antibody [NBP1-52193] - Staining of MUC4 in mouse prostate")
![Western Blot: MUC4 Antibody [NBP1-52193] - Analysis of MUC4 beta in MCF7 whole cell extract (A) and HeLa whole cell extract (B).](http://images.novusbio.com/fullsize/MUC-4-Antibody-Western-Blot-NBP1-52193-img0007.jpg "Western Blot: MUC4 Antibody [NBP1-52193] - Analysis of MUC4 beta in MCF7 whole cell extract (A) and HeLa whole cell extract (B).")
![Simple Western: MUC4 Antibody [NBP1-52193] - Image shows a specific band for MUC4 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/MUC-4-Antibody-Simple-Western-NBP1-52193-img0009.jpg "Simple Western: MUC4 Antibody [NBP1-52193] - Image shows a specific band for MUC4 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![SDS-PAGE ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/ErbB2_1129-ER_70.jpg)
![Bioactivity ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/1129-er_recombinant-human-erbb2-her2-fc-chimera-protein-cf-bioactivity-7122020142841.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![SDS-PAGE EGF [Unconjugated]](https://images.novusbio.com/images2/EGF_236-EG_234.jpg)
![Bioactivity EGF [Unconjugated]](https://images.novusbio.com/images2/EGF_236EG_1570.jpg)
![Cell Culture EGF [Unconjugated]](https://images.novusbio.com/images/236-eg_recombinant-human-egf-protein-cf-bioactivity-25202394053.jpg)
